Long non-coding RNA NEAT1 cannot be used as a diagnostic and prognostic biomarker in patients with locally advanced rectal cancer
Само за регистроване кориснике
2023
Аутори
Erić, K.Rosić, Jovana
Miladinov, Marko
Dragičević, Sandra
Barišić, Goran
Marković, V.
Zeljić, Katarina
Конференцијски прилог (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Background & objectives: The NEAT1 (Nuclear Paraspeckle Assembly Transcript 1) gene encodes a long non-coding RNA that is deregulated in carcinomas of the gastrointestinal tract. Diagnostic and predictive potential of NEAT1 was investigated in patients with locally
advanced rectal cancer.
Methods: The study group consisted of 19 patients with rectal cancer
treated with neoadjuvant chemoradiotherapy (nCRT). RNA was isolated with TRIzol reagent from samples of rectal cancer and noncancerous tissue before and after nCRT. The relative expression level of
NEAT1 normalised to GAPDH was determined by qRT-PCR method.
Results: Expression of NEAT1 did not difer between rectal cancer and
noncancerous tissue before nCRT (p=0.953) and cancer and noncancerous tissue after nCRT (p=0.210). There was no diference in NEAT1
expression between tumour tissue before and after nCRT (p=0.079).
NEAT1 was signifcantly higher in noncancerous tissue before than
after nCRT (p=0.005). Therapy responders (T...RG1, TRG2) and nonresponders (TRG3, TRG4) did not difer in NEAT1 levels in tumour
tissue before (p=0.790) and after nCRT (p=0.352). NEAT1 expression
in rectal cancer tissue before nCRT cannot be used as a biomarker to
distinguish responders from non-responders (AUC=0.559, 95%CI=0-
1, p=0.790). Demographic and clinicopathological characteristics were
not associated with NEAT1 expression in rectal cancer tissue.
Conclusion: The obtained results suggest that the long noncoding
RNA NEAT1 cannot be considered as a biomarker with diagnostic
potential or for predicting response to nCRT in patients with rectal
cancer. Validation of the current results in a larger group of patients
with locally advanced rectal cancer is warranted.
Извор:
Virchows Archiv, 35th European Congress of Pathology Pathology – a bridge between Science and Medicine, 9 – 13 September, 2023, 483, Supplement 1, S307-S307Издавач:
- Springer
Финансирање / пројекти:
- Strategic Project of the Serbian Academy of Sciences and Arts Molecular basis of response to chemioradiotherapy in rectal cancer - MOHERATEKA, F-69
Колекције
Институција/група
Institut za molekularnu genetiku i genetičko inženjerstvoTY - CONF AU - Erić, K. AU - Rosić, Jovana AU - Miladinov, Marko AU - Dragičević, Sandra AU - Barišić, Goran AU - Marković, V. AU - Zeljić, Katarina PY - 2023 UR - https://imagine.imgge.bg.ac.rs/handle/123456789/2081 AB - Background & objectives: The NEAT1 (Nuclear Paraspeckle Assembly Transcript 1) gene encodes a long non-coding RNA that is deregulated in carcinomas of the gastrointestinal tract. Diagnostic and predictive potential of NEAT1 was investigated in patients with locally advanced rectal cancer. Methods: The study group consisted of 19 patients with rectal cancer treated with neoadjuvant chemoradiotherapy (nCRT). RNA was isolated with TRIzol reagent from samples of rectal cancer and noncancerous tissue before and after nCRT. The relative expression level of NEAT1 normalised to GAPDH was determined by qRT-PCR method. Results: Expression of NEAT1 did not difer between rectal cancer and noncancerous tissue before nCRT (p=0.953) and cancer and noncancerous tissue after nCRT (p=0.210). There was no diference in NEAT1 expression between tumour tissue before and after nCRT (p=0.079). NEAT1 was signifcantly higher in noncancerous tissue before than after nCRT (p=0.005). Therapy responders (TRG1, TRG2) and nonresponders (TRG3, TRG4) did not difer in NEAT1 levels in tumour tissue before (p=0.790) and after nCRT (p=0.352). NEAT1 expression in rectal cancer tissue before nCRT cannot be used as a biomarker to distinguish responders from non-responders (AUC=0.559, 95%CI=0- 1, p=0.790). Demographic and clinicopathological characteristics were not associated with NEAT1 expression in rectal cancer tissue. Conclusion: The obtained results suggest that the long noncoding RNA NEAT1 cannot be considered as a biomarker with diagnostic potential or for predicting response to nCRT in patients with rectal cancer. Validation of the current results in a larger group of patients with locally advanced rectal cancer is warranted. PB - Springer C3 - Virchows Archiv, 35th European Congress of Pathology Pathology – a bridge between Science and Medicine, 9 – 13 September T1 - Long non-coding RNA NEAT1 cannot be used as a diagnostic and prognostic biomarker in patients with locally advanced rectal cancer EP - S307 IS - Supplement 1 SP - S307 SP - E-PS-15-011 VL - 483 UR - https://hdl.handle.net/21.15107/rcub_imagine_2081 ER -
@conference{ author = "Erić, K. and Rosić, Jovana and Miladinov, Marko and Dragičević, Sandra and Barišić, Goran and Marković, V. and Zeljić, Katarina", year = "2023", abstract = "Background & objectives: The NEAT1 (Nuclear Paraspeckle Assembly Transcript 1) gene encodes a long non-coding RNA that is deregulated in carcinomas of the gastrointestinal tract. Diagnostic and predictive potential of NEAT1 was investigated in patients with locally advanced rectal cancer. Methods: The study group consisted of 19 patients with rectal cancer treated with neoadjuvant chemoradiotherapy (nCRT). RNA was isolated with TRIzol reagent from samples of rectal cancer and noncancerous tissue before and after nCRT. The relative expression level of NEAT1 normalised to GAPDH was determined by qRT-PCR method. Results: Expression of NEAT1 did not difer between rectal cancer and noncancerous tissue before nCRT (p=0.953) and cancer and noncancerous tissue after nCRT (p=0.210). There was no diference in NEAT1 expression between tumour tissue before and after nCRT (p=0.079). NEAT1 was signifcantly higher in noncancerous tissue before than after nCRT (p=0.005). Therapy responders (TRG1, TRG2) and nonresponders (TRG3, TRG4) did not difer in NEAT1 levels in tumour tissue before (p=0.790) and after nCRT (p=0.352). NEAT1 expression in rectal cancer tissue before nCRT cannot be used as a biomarker to distinguish responders from non-responders (AUC=0.559, 95%CI=0- 1, p=0.790). Demographic and clinicopathological characteristics were not associated with NEAT1 expression in rectal cancer tissue. Conclusion: The obtained results suggest that the long noncoding RNA NEAT1 cannot be considered as a biomarker with diagnostic potential or for predicting response to nCRT in patients with rectal cancer. Validation of the current results in a larger group of patients with locally advanced rectal cancer is warranted.", publisher = "Springer", journal = "Virchows Archiv, 35th European Congress of Pathology Pathology – a bridge between Science and Medicine, 9 – 13 September", title = "Long non-coding RNA NEAT1 cannot be used as a diagnostic and prognostic biomarker in patients with locally advanced rectal cancer", pages = "S307-S307-E-PS-15-011", number = "Supplement 1", volume = "483", url = "https://hdl.handle.net/21.15107/rcub_imagine_2081" }
Erić, K., Rosić, J., Miladinov, M., Dragičević, S., Barišić, G., Marković, V.,& Zeljić, K.. (2023). Long non-coding RNA NEAT1 cannot be used as a diagnostic and prognostic biomarker in patients with locally advanced rectal cancer. in Virchows Archiv, 35th European Congress of Pathology Pathology – a bridge between Science and Medicine, 9 – 13 September Springer., 483(Supplement 1), S307-S307. https://hdl.handle.net/21.15107/rcub_imagine_2081
Erić K, Rosić J, Miladinov M, Dragičević S, Barišić G, Marković V, Zeljić K. Long non-coding RNA NEAT1 cannot be used as a diagnostic and prognostic biomarker in patients with locally advanced rectal cancer. in Virchows Archiv, 35th European Congress of Pathology Pathology – a bridge between Science and Medicine, 9 – 13 September. 2023;483(Supplement 1):S307-S307. https://hdl.handle.net/21.15107/rcub_imagine_2081 .
Erić, K., Rosić, Jovana, Miladinov, Marko, Dragičević, Sandra, Barišić, Goran, Marković, V., Zeljić, Katarina, "Long non-coding RNA NEAT1 cannot be used as a diagnostic and prognostic biomarker in patients with locally advanced rectal cancer" in Virchows Archiv, 35th European Congress of Pathology Pathology – a bridge between Science and Medicine, 9 – 13 September, 483, no. Supplement 1 (2023):S307-S307, https://hdl.handle.net/21.15107/rcub_imagine_2081 .