Small Cellular Particles from European Spruce Needle Homogenate
Аутори
Jeran, MarkoRomolo, Anna
Spasovski, Vesna
Hočevar, Matej
Novak, Urban
Štukelj, Roman
Šuštar, Vid
Kisovec, Matic
Bedina Zavec, Apolonija
Kogej, Ksenija
Iglič, Aleš
Trebše, Polonca
Kralj-Iglič, Veronika
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Small cellular particles (SCPs) are being considered for their role in cell-to-cell communication. We harvested and characterized SCPs from spruce needle homogenate. SCPs were isolated by differential ultracentrifugation. They were imaged by scanning electron microscope (SEM) and cryogenic transmission electron microscope (cryo TEM), assessed for their number density and hydrodynamic diameter by interferometric light microscopy (ILM) and flow cytometry (FCM), total phenolic content (TPC) by UV-vis spectroscopy, and terpene content by gas chromatography-mass spectrometry (GC-MS). The supernatant after ultracentrifugation at 50,000× g contained bilayer-enclosed vesicles whereas in the isolate we observed small particles of other types and only a few vesicles. The number density of cell-sized particles (CSPs) (larger than 2 μm) and meso-sized particles (MSPs) (cca 400 nm–2 µm) was about four orders of magnitude lower than the number density of SCPs (sized below 500 nm). The average hydrod...ynamic diameter of SCPs measured in 10,029 SCPs was 161 ± 133 nm. TCP decreased considerably due to 5-day aging. Volatile terpenoid content was found in the pellet after 300× g. The above results indicate that spruce needle homogenate is a source of vesicles to be explored for potential delivery use.
Кључне речи:
Picea abies / cell-to-cell communication / drug delivery / exosomes / extracellular particles / extracellular vesicles / interferometric light microscopy / small cellular particles / spruce needlesИзвор:
International Journal of Molecular Sciences, 2023, 24, 5, 4349-Издавач:
- MDPI
Финансирање / пројекти:
- Slovenian Research Agency, grant number P1-0201
- Slovenian Research Agency, grant number P2- 0132
- Slovenian Research Agency, grant number P2-0232
- Slovenian Research Agency, grant number P3-0388
- Slovenian Research Agency, grant number P1-0391
- Slovenian Research Agency, grant number J2-4447
- Slovenian Research Agency, grant number J3-3066
- Slovenian Research Agency, grant number L3-2621
- Slovenian Research Agency, grant number IO-0003
- National Research, Development and Innovation Office (Hungary) SNN 138407
Институција/група
Institut za molekularnu genetiku i genetičko inženjerstvoTY - JOUR AU - Jeran, Marko AU - Romolo, Anna AU - Spasovski, Vesna AU - Hočevar, Matej AU - Novak, Urban AU - Štukelj, Roman AU - Šuštar, Vid AU - Kisovec, Matic AU - Bedina Zavec, Apolonija AU - Kogej, Ksenija AU - Iglič, Aleš AU - Trebše, Polonca AU - Kralj-Iglič, Veronika PY - 2023 UR - https://imagine.imgge.bg.ac.rs/handle/123456789/2086 AB - Small cellular particles (SCPs) are being considered for their role in cell-to-cell communication. We harvested and characterized SCPs from spruce needle homogenate. SCPs were isolated by differential ultracentrifugation. They were imaged by scanning electron microscope (SEM) and cryogenic transmission electron microscope (cryo TEM), assessed for their number density and hydrodynamic diameter by interferometric light microscopy (ILM) and flow cytometry (FCM), total phenolic content (TPC) by UV-vis spectroscopy, and terpene content by gas chromatography-mass spectrometry (GC-MS). The supernatant after ultracentrifugation at 50,000× g contained bilayer-enclosed vesicles whereas in the isolate we observed small particles of other types and only a few vesicles. The number density of cell-sized particles (CSPs) (larger than 2 μm) and meso-sized particles (MSPs) (cca 400 nm–2 µm) was about four orders of magnitude lower than the number density of SCPs (sized below 500 nm). The average hydrodynamic diameter of SCPs measured in 10,029 SCPs was 161 ± 133 nm. TCP decreased considerably due to 5-day aging. Volatile terpenoid content was found in the pellet after 300× g. The above results indicate that spruce needle homogenate is a source of vesicles to be explored for potential delivery use. PB - MDPI T2 - International Journal of Molecular Sciences T1 - Small Cellular Particles from European Spruce Needle Homogenate IS - 5 SP - 4349 VL - 24 DO - 10.3390/ijms24054349 ER -
@article{ author = "Jeran, Marko and Romolo, Anna and Spasovski, Vesna and Hočevar, Matej and Novak, Urban and Štukelj, Roman and Šuštar, Vid and Kisovec, Matic and Bedina Zavec, Apolonija and Kogej, Ksenija and Iglič, Aleš and Trebše, Polonca and Kralj-Iglič, Veronika", year = "2023", abstract = "Small cellular particles (SCPs) are being considered for their role in cell-to-cell communication. We harvested and characterized SCPs from spruce needle homogenate. SCPs were isolated by differential ultracentrifugation. They were imaged by scanning electron microscope (SEM) and cryogenic transmission electron microscope (cryo TEM), assessed for their number density and hydrodynamic diameter by interferometric light microscopy (ILM) and flow cytometry (FCM), total phenolic content (TPC) by UV-vis spectroscopy, and terpene content by gas chromatography-mass spectrometry (GC-MS). The supernatant after ultracentrifugation at 50,000× g contained bilayer-enclosed vesicles whereas in the isolate we observed small particles of other types and only a few vesicles. The number density of cell-sized particles (CSPs) (larger than 2 μm) and meso-sized particles (MSPs) (cca 400 nm–2 µm) was about four orders of magnitude lower than the number density of SCPs (sized below 500 nm). The average hydrodynamic diameter of SCPs measured in 10,029 SCPs was 161 ± 133 nm. TCP decreased considerably due to 5-day aging. Volatile terpenoid content was found in the pellet after 300× g. The above results indicate that spruce needle homogenate is a source of vesicles to be explored for potential delivery use.", publisher = "MDPI", journal = "International Journal of Molecular Sciences", title = "Small Cellular Particles from European Spruce Needle Homogenate", number = "5", pages = "4349", volume = "24", doi = "10.3390/ijms24054349" }
Jeran, M., Romolo, A., Spasovski, V., Hočevar, M., Novak, U., Štukelj, R., Šuštar, V., Kisovec, M., Bedina Zavec, A., Kogej, K., Iglič, A., Trebše, P.,& Kralj-Iglič, V.. (2023). Small Cellular Particles from European Spruce Needle Homogenate. in International Journal of Molecular Sciences MDPI., 24(5), 4349. https://doi.org/10.3390/ijms24054349
Jeran M, Romolo A, Spasovski V, Hočevar M, Novak U, Štukelj R, Šuštar V, Kisovec M, Bedina Zavec A, Kogej K, Iglič A, Trebše P, Kralj-Iglič V. Small Cellular Particles from European Spruce Needle Homogenate. in International Journal of Molecular Sciences. 2023;24(5):4349. doi:10.3390/ijms24054349 .
Jeran, Marko, Romolo, Anna, Spasovski, Vesna, Hočevar, Matej, Novak, Urban, Štukelj, Roman, Šuštar, Vid, Kisovec, Matic, Bedina Zavec, Apolonija, Kogej, Ksenija, Iglič, Aleš, Trebše, Polonca, Kralj-Iglič, Veronika, "Small Cellular Particles from European Spruce Needle Homogenate" in International Journal of Molecular Sciences, 24, no. 5 (2023):4349, https://doi.org/10.3390/ijms24054349 . .