Lung microbiota changes during pulmonary Aspergillus fumigatus infection in rats
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2023
Authors
Popović, DušankaKulas, Jelena
Tucovic, Dina
Popov Aleksandrov, Aleksandra
Glamoclija, Jasmina
Sokovic Bajic, Svetlana
Tolinački, Maja
Golić, Nataša
Mirkov, Ivana
Article (Published version)
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Show full item recordAbstract
Since the realization that the lungs are not sterile but are normally inhabited by various bacterial species, studies have been conducted to define healthy lung microbiota and to investigate whether it changes during lung diseases, infections, and inflammation. Using next-generation sequencing, we investigated bacterial microbiota from whole lungs in two rat strains (previously shown to differ in gut microbiota composition) in a healthy state and during pulmonary infection caused by the opportunistic fungus Aspergillus fumigatus. No differences in alpha diversity indices and microbial composition between DA and AO rats before infection were noted. Fungal infection caused dysbiosis in both rat strains, characterized by increased alpha diversity indices and unchanged beta diversity. The relative abundance of genera and species was increased in DA but decreased in AO rats during infection. Changes in lung microbiota coincided with inflammation (in both rat strains) and oxidative stress (i...n DA rats). Disparate response of lung microbiota in DA and AO rats to pulmonary fungal infection might render these two rat strains differentially susceptible to a subsequent inflammatory insult.
Keywords:
Inflammation / lung infection / Oxidative stress / Rats / Whole lung tissue microbiotaSource:
Microbes and Infection, 2023, 105186-Funding / projects:
- Ministry of Science, Technological Development and Innovation of the Republic of Serbia, institutional funding - 200042 (University of Belgrade, Institute of Molecular Genetics and Genetic Engineering) (RS-MESTD-inst-2020-200042)
- Ministry of Science, Technological Development and Innovation of the Republic of Serbia, institutional funding - 200007 (University of Belgrade, Institute for Biological Research 'Siniša Stanković') (RS-MESTD-inst-2020-200007)
URI
https://www.sciencedirect.com/science/article/pii/S1286457923000898https://imagine.imgge.bg.ac.rs/handle/123456789/2094
Institution/Community
Institut za molekularnu genetiku i genetičko inženjerstvoTY - JOUR AU - Popović, Dušanka AU - Kulas, Jelena AU - Tucovic, Dina AU - Popov Aleksandrov, Aleksandra AU - Glamoclija, Jasmina AU - Sokovic Bajic, Svetlana AU - Tolinački, Maja AU - Golić, Nataša AU - Mirkov, Ivana PY - 2023 UR - https://www.sciencedirect.com/science/article/pii/S1286457923000898 UR - https://imagine.imgge.bg.ac.rs/handle/123456789/2094 AB - Since the realization that the lungs are not sterile but are normally inhabited by various bacterial species, studies have been conducted to define healthy lung microbiota and to investigate whether it changes during lung diseases, infections, and inflammation. Using next-generation sequencing, we investigated bacterial microbiota from whole lungs in two rat strains (previously shown to differ in gut microbiota composition) in a healthy state and during pulmonary infection caused by the opportunistic fungus Aspergillus fumigatus. No differences in alpha diversity indices and microbial composition between DA and AO rats before infection were noted. Fungal infection caused dysbiosis in both rat strains, characterized by increased alpha diversity indices and unchanged beta diversity. The relative abundance of genera and species was increased in DA but decreased in AO rats during infection. Changes in lung microbiota coincided with inflammation (in both rat strains) and oxidative stress (in DA rats). Disparate response of lung microbiota in DA and AO rats to pulmonary fungal infection might render these two rat strains differentially susceptible to a subsequent inflammatory insult. T2 - Microbes and Infection T2 - Microbes and InfectionMicrobes and Infection T1 - Lung microbiota changes during pulmonary Aspergillus fumigatus infection in rats SP - 105186 DO - 10.1016/j.micinf.2023.105186 ER -
@article{ author = "Popović, Dušanka and Kulas, Jelena and Tucovic, Dina and Popov Aleksandrov, Aleksandra and Glamoclija, Jasmina and Sokovic Bajic, Svetlana and Tolinački, Maja and Golić, Nataša and Mirkov, Ivana", year = "2023", abstract = "Since the realization that the lungs are not sterile but are normally inhabited by various bacterial species, studies have been conducted to define healthy lung microbiota and to investigate whether it changes during lung diseases, infections, and inflammation. Using next-generation sequencing, we investigated bacterial microbiota from whole lungs in two rat strains (previously shown to differ in gut microbiota composition) in a healthy state and during pulmonary infection caused by the opportunistic fungus Aspergillus fumigatus. No differences in alpha diversity indices and microbial composition between DA and AO rats before infection were noted. Fungal infection caused dysbiosis in both rat strains, characterized by increased alpha diversity indices and unchanged beta diversity. The relative abundance of genera and species was increased in DA but decreased in AO rats during infection. Changes in lung microbiota coincided with inflammation (in both rat strains) and oxidative stress (in DA rats). Disparate response of lung microbiota in DA and AO rats to pulmonary fungal infection might render these two rat strains differentially susceptible to a subsequent inflammatory insult.", journal = "Microbes and Infection, Microbes and InfectionMicrobes and Infection", title = "Lung microbiota changes during pulmonary Aspergillus fumigatus infection in rats", pages = "105186", doi = "10.1016/j.micinf.2023.105186" }
Popović, D., Kulas, J., Tucovic, D., Popov Aleksandrov, A., Glamoclija, J., Sokovic Bajic, S., Tolinački, M., Golić, N.,& Mirkov, I.. (2023). Lung microbiota changes during pulmonary Aspergillus fumigatus infection in rats. in Microbes and Infection, 105186. https://doi.org/10.1016/j.micinf.2023.105186
Popović D, Kulas J, Tucovic D, Popov Aleksandrov A, Glamoclija J, Sokovic Bajic S, Tolinački M, Golić N, Mirkov I. Lung microbiota changes during pulmonary Aspergillus fumigatus infection in rats. in Microbes and Infection. 2023;:105186. doi:10.1016/j.micinf.2023.105186 .
Popović, Dušanka, Kulas, Jelena, Tucovic, Dina, Popov Aleksandrov, Aleksandra, Glamoclija, Jasmina, Sokovic Bajic, Svetlana, Tolinački, Maja, Golić, Nataša, Mirkov, Ivana, "Lung microbiota changes during pulmonary Aspergillus fumigatus infection in rats" in Microbes and Infection (2023):105186, https://doi.org/10.1016/j.micinf.2023.105186 . .