Bacteria in drinking and bathing mineral waters of Serbia with polymer-degrading potential
Аутори
Ćirić, MilicaŠaraba, Vladimir
Budin, Clémence
Boer de, Tjalf
Milovanović, Jelena
Nikodinović-Runić, Jasmina
Остала ауторства
Marković, ZoranFilipović, Nenad
Конференцијски прилог (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Three mineral water occurrences, captured by wells with a depth of 6.5-442.5 m and used for drinking
and bathing purposes, were sampled and cultivated under conditions favouring the growth of anaerobic,
microaerophilic or CO2 bacteria, in order to capture predominantly anaerobic portion of the bacteriome, which is
dominant in water and soils. Cultivated bacteria were identified by next-generation 16S sequencing and their
biotechnological potential in plastics and lignocellulose degradation was explored. Most abundant genera
detected in examined samples mainly belong to facultative anaerobes that are common representatives of water
and soil environments. In total, 17 genera were detected with a relative abundance over 1% in all three samples,
including Aeromonas, Exiguobacterium, Comamonas and Acinetobacter. Half of the screened isolates demonstrated
growth on at least one plastic or lignocellulosic polymer, with one isolate demonstrating growth on all tested
substrates, one de...monstrating carboxymethyl cellulose- and one arabinoxylan-degrading ability. Some of the
representatives of genera identified with high relative abundance in mineral water samples, such as Aeromonas,
Klebsiella, Escherichia, Salmonella, Enterobacter, Pseudomonas and Staphylococcus, have been previously documented
to have pathogenic potential. Due to the use of investigated mineral waters for drinking and bathing, the health
risk from such bacteria in these occurrences needs to be continuously monitored, while, on the other hand,
mineral waters deserve special attention in the future from the aspect of screening for biotechnologically
relevant enzymes.
Кључне речи:
mineral water / bacteria / 16S rDNA sequencing / SerbiaИзвор:
2nd International Conference on Chemo and Bioinformatics, 2023, 191-195Издавач:
- Kragujevac : Institute for Information Technologies
Финансирање / пројекти:
- EU H2020 Research and Innovation Programme (grant agreement No. 870292, BioICEP)
- Министарство науке, технолошког развоја и иновација Републике Србије, институционално финансирање - 200042 (Универзитет у Београду, Институт за молекуларну генетику и генетичко инжењерство) (RS-MESTD-inst-2020-200042)
- The 16S rDNA sequence datasets for cultivated groundwater samples are deposited in the NCBI BioProject database (PRJNA977830)
Напомена:
- 2nd International Conference on Chemo and BioInformatics, ICCBIKG 2023, September 28-29, 2023 Kragujevac
Институција/група
Institut za molekularnu genetiku i genetičko inženjerstvoTY - CONF AU - Ćirić, Milica AU - Šaraba, Vladimir AU - Budin, Clémence AU - Boer de, Tjalf AU - Milovanović, Jelena AU - Nikodinović-Runić, Jasmina PY - 2023 UR - https://imagine.imgge.bg.ac.rs/handle/123456789/2105 AB - Three mineral water occurrences, captured by wells with a depth of 6.5-442.5 m and used for drinking and bathing purposes, were sampled and cultivated under conditions favouring the growth of anaerobic, microaerophilic or CO2 bacteria, in order to capture predominantly anaerobic portion of the bacteriome, which is dominant in water and soils. Cultivated bacteria were identified by next-generation 16S sequencing and their biotechnological potential in plastics and lignocellulose degradation was explored. Most abundant genera detected in examined samples mainly belong to facultative anaerobes that are common representatives of water and soil environments. In total, 17 genera were detected with a relative abundance over 1% in all three samples, including Aeromonas, Exiguobacterium, Comamonas and Acinetobacter. Half of the screened isolates demonstrated growth on at least one plastic or lignocellulosic polymer, with one isolate demonstrating growth on all tested substrates, one demonstrating carboxymethyl cellulose- and one arabinoxylan-degrading ability. Some of the representatives of genera identified with high relative abundance in mineral water samples, such as Aeromonas, Klebsiella, Escherichia, Salmonella, Enterobacter, Pseudomonas and Staphylococcus, have been previously documented to have pathogenic potential. Due to the use of investigated mineral waters for drinking and bathing, the health risk from such bacteria in these occurrences needs to be continuously monitored, while, on the other hand, mineral waters deserve special attention in the future from the aspect of screening for biotechnologically relevant enzymes. PB - Kragujevac : Institute for Information Technologies C3 - 2nd International Conference on Chemo and Bioinformatics T1 - Bacteria in drinking and bathing mineral waters of Serbia with polymer-degrading potential EP - 195 SP - 191 DO - 10.46793/ICCBI23.091C ER -
@conference{ author = "Ćirić, Milica and Šaraba, Vladimir and Budin, Clémence and Boer de, Tjalf and Milovanović, Jelena and Nikodinović-Runić, Jasmina", year = "2023", abstract = "Three mineral water occurrences, captured by wells with a depth of 6.5-442.5 m and used for drinking and bathing purposes, were sampled and cultivated under conditions favouring the growth of anaerobic, microaerophilic or CO2 bacteria, in order to capture predominantly anaerobic portion of the bacteriome, which is dominant in water and soils. Cultivated bacteria were identified by next-generation 16S sequencing and their biotechnological potential in plastics and lignocellulose degradation was explored. Most abundant genera detected in examined samples mainly belong to facultative anaerobes that are common representatives of water and soil environments. In total, 17 genera were detected with a relative abundance over 1% in all three samples, including Aeromonas, Exiguobacterium, Comamonas and Acinetobacter. Half of the screened isolates demonstrated growth on at least one plastic or lignocellulosic polymer, with one isolate demonstrating growth on all tested substrates, one demonstrating carboxymethyl cellulose- and one arabinoxylan-degrading ability. Some of the representatives of genera identified with high relative abundance in mineral water samples, such as Aeromonas, Klebsiella, Escherichia, Salmonella, Enterobacter, Pseudomonas and Staphylococcus, have been previously documented to have pathogenic potential. Due to the use of investigated mineral waters for drinking and bathing, the health risk from such bacteria in these occurrences needs to be continuously monitored, while, on the other hand, mineral waters deserve special attention in the future from the aspect of screening for biotechnologically relevant enzymes.", publisher = "Kragujevac : Institute for Information Technologies", journal = "2nd International Conference on Chemo and Bioinformatics", title = "Bacteria in drinking and bathing mineral waters of Serbia with polymer-degrading potential", pages = "195-191", doi = "10.46793/ICCBI23.091C" }
Ćirić, M., Šaraba, V., Budin, C., Boer de, T., Milovanović, J.,& Nikodinović-Runić, J.. (2023). Bacteria in drinking and bathing mineral waters of Serbia with polymer-degrading potential. in 2nd International Conference on Chemo and Bioinformatics Kragujevac : Institute for Information Technologies., 191-195. https://doi.org/10.46793/ICCBI23.091C
Ćirić M, Šaraba V, Budin C, Boer de T, Milovanović J, Nikodinović-Runić J. Bacteria in drinking and bathing mineral waters of Serbia with polymer-degrading potential. in 2nd International Conference on Chemo and Bioinformatics. 2023;:191-195. doi:10.46793/ICCBI23.091C .
Ćirić, Milica, Šaraba, Vladimir, Budin, Clémence, Boer de, Tjalf, Milovanović, Jelena, Nikodinović-Runić, Jasmina, "Bacteria in drinking and bathing mineral waters of Serbia with polymer-degrading potential" in 2nd International Conference on Chemo and Bioinformatics (2023):191-195, https://doi.org/10.46793/ICCBI23.091C . .