Determination of muscle fiber types expressing ANKRD2
Аутори
Novković, MirjanaVasić, Marko
Jasnić, Jovana
Milošević, Emilija
Milovanović, Mina
Savić, Slobodan
Kojić, Snežana
Конференцијски прилог (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Introduction: Ankyrin Repeat Domain 2 (ANKRD2) is expressed in skeletal muscle, where plays a role in
muscle development, differentiation and adaptation to stress. Human skeletal muscle consists of three
major fiber types: type 1 (slow-twitch, oxidative), type 2A (fast-twitch, oxidative) and type 2X (fast-twitch,
glycolytic). ANKRD2 is reported to be primarily expressed in type 1 myofibers. However, recent findings
on human single myofibers and our study of chicken muscles have shown that this protein may also be
expressed in type 2A fibers. Hence, our objective was to examine whether ANKRD2 is present in human
fast, type 2A muscle fibers using immunohistochemistry.
Methods: Samples of large leg musclessoleus, gastrocnemius, vastusintermedius and vastuslateralis were
obtained from human cadaveric tissue. Serial cryosections were independently stained with anti-ANKRD2
and antibodies for different myosin heavy chain isoforms (6H1 for type 2X, BF35 for type 1 and 2A, antiMHCs for... type 1 and anti-MHCf for type 2A and 2X fibers). Immunostained tissues were analyzed by fluorescent microscopy.
Results: In addition to slow, type 1, ANKRD2 wasfound expressed in fast, type 2A myofibers, which both
have oxidative metabolism. Further, we did not observe ANDRD2 expression in glycolytic, type 2X
myiofibers. This pattern of ANKRD2 expression was consistent across all examined muscles.
Conclusion: Our resultsimplicate that the regulatory mechanism of ANKRD2 expression in human skeletal muscle is associated with oxidative metabolism, rather than muscle contraction speed.
Кључне речи:
ANKRD2 / muscle fibers types / protein expression / immunohistochemistryИзвор:
CoMBoS2 – the Second Congress of Molecular Biologists of Serbia, Abstract Book – Trends in Molecular Biology, Special issue 06-08 October 2023, Belgrade, Serbia, 2023, 155-155Издавач:
- Institute of Molecular Genetics and Genetic Engineering (IMGGE), University of Belgrade
Финансирање / пројекти:
- Министарство науке, технолошког развоја и иновација Републике Србије, институционално финансирање - 200042 (Универзитет у Београду, Институт за молекуларну генетику и генетичко инжењерство) (RS-MESTD-inst-2020-200042)
Колекције
Институција/група
Institut za molekularnu genetiku i genetičko inženjerstvoTY - CONF AU - Novković, Mirjana AU - Vasić, Marko AU - Jasnić, Jovana AU - Milošević, Emilija AU - Milovanović, Mina AU - Savić, Slobodan AU - Kojić, Snežana PY - 2023 UR - https://imagine.imgge.bg.ac.rs/handle/123456789/2114 AB - Introduction: Ankyrin Repeat Domain 2 (ANKRD2) is expressed in skeletal muscle, where plays a role in muscle development, differentiation and adaptation to stress. Human skeletal muscle consists of three major fiber types: type 1 (slow-twitch, oxidative), type 2A (fast-twitch, oxidative) and type 2X (fast-twitch, glycolytic). ANKRD2 is reported to be primarily expressed in type 1 myofibers. However, recent findings on human single myofibers and our study of chicken muscles have shown that this protein may also be expressed in type 2A fibers. Hence, our objective was to examine whether ANKRD2 is present in human fast, type 2A muscle fibers using immunohistochemistry. Methods: Samples of large leg musclessoleus, gastrocnemius, vastusintermedius and vastuslateralis were obtained from human cadaveric tissue. Serial cryosections were independently stained with anti-ANKRD2 and antibodies for different myosin heavy chain isoforms (6H1 for type 2X, BF35 for type 1 and 2A, antiMHCs for type 1 and anti-MHCf for type 2A and 2X fibers). Immunostained tissues were analyzed by fluorescent microscopy. Results: In addition to slow, type 1, ANKRD2 wasfound expressed in fast, type 2A myofibers, which both have oxidative metabolism. Further, we did not observe ANDRD2 expression in glycolytic, type 2X myiofibers. This pattern of ANKRD2 expression was consistent across all examined muscles. Conclusion: Our resultsimplicate that the regulatory mechanism of ANKRD2 expression in human skeletal muscle is associated with oxidative metabolism, rather than muscle contraction speed. PB - Institute of Molecular Genetics and Genetic Engineering (IMGGE), University of Belgrade C3 - CoMBoS2 – the Second Congress of Molecular Biologists of Serbia, Abstract Book – Trends in Molecular Biology, Special issue 06-08 October 2023, Belgrade, Serbia T1 - Determination of muscle fiber types expressing ANKRD2 EP - 155 SP - 155 UR - https://hdl.handle.net/21.15107/rcub_imagine_2114 ER -
@conference{ author = "Novković, Mirjana and Vasić, Marko and Jasnić, Jovana and Milošević, Emilija and Milovanović, Mina and Savić, Slobodan and Kojić, Snežana", year = "2023", abstract = "Introduction: Ankyrin Repeat Domain 2 (ANKRD2) is expressed in skeletal muscle, where plays a role in muscle development, differentiation and adaptation to stress. Human skeletal muscle consists of three major fiber types: type 1 (slow-twitch, oxidative), type 2A (fast-twitch, oxidative) and type 2X (fast-twitch, glycolytic). ANKRD2 is reported to be primarily expressed in type 1 myofibers. However, recent findings on human single myofibers and our study of chicken muscles have shown that this protein may also be expressed in type 2A fibers. Hence, our objective was to examine whether ANKRD2 is present in human fast, type 2A muscle fibers using immunohistochemistry. Methods: Samples of large leg musclessoleus, gastrocnemius, vastusintermedius and vastuslateralis were obtained from human cadaveric tissue. Serial cryosections were independently stained with anti-ANKRD2 and antibodies for different myosin heavy chain isoforms (6H1 for type 2X, BF35 for type 1 and 2A, antiMHCs for type 1 and anti-MHCf for type 2A and 2X fibers). Immunostained tissues were analyzed by fluorescent microscopy. Results: In addition to slow, type 1, ANKRD2 wasfound expressed in fast, type 2A myofibers, which both have oxidative metabolism. Further, we did not observe ANDRD2 expression in glycolytic, type 2X myiofibers. This pattern of ANKRD2 expression was consistent across all examined muscles. Conclusion: Our resultsimplicate that the regulatory mechanism of ANKRD2 expression in human skeletal muscle is associated with oxidative metabolism, rather than muscle contraction speed.", publisher = "Institute of Molecular Genetics and Genetic Engineering (IMGGE), University of Belgrade", journal = "CoMBoS2 – the Second Congress of Molecular Biologists of Serbia, Abstract Book – Trends in Molecular Biology, Special issue 06-08 October 2023, Belgrade, Serbia", title = "Determination of muscle fiber types expressing ANKRD2", pages = "155-155", url = "https://hdl.handle.net/21.15107/rcub_imagine_2114" }
Novković, M., Vasić, M., Jasnić, J., Milošević, E., Milovanović, M., Savić, S.,& Kojić, S.. (2023). Determination of muscle fiber types expressing ANKRD2. in CoMBoS2 – the Second Congress of Molecular Biologists of Serbia, Abstract Book – Trends in Molecular Biology, Special issue 06-08 October 2023, Belgrade, Serbia Institute of Molecular Genetics and Genetic Engineering (IMGGE), University of Belgrade., 155-155. https://hdl.handle.net/21.15107/rcub_imagine_2114
Novković M, Vasić M, Jasnić J, Milošević E, Milovanović M, Savić S, Kojić S. Determination of muscle fiber types expressing ANKRD2. in CoMBoS2 – the Second Congress of Molecular Biologists of Serbia, Abstract Book – Trends in Molecular Biology, Special issue 06-08 October 2023, Belgrade, Serbia. 2023;:155-155. https://hdl.handle.net/21.15107/rcub_imagine_2114 .
Novković, Mirjana, Vasić, Marko, Jasnić, Jovana, Milošević, Emilija, Milovanović, Mina, Savić, Slobodan, Kojić, Snežana, "Determination of muscle fiber types expressing ANKRD2" in CoMBoS2 – the Second Congress of Molecular Biologists of Serbia, Abstract Book – Trends in Molecular Biology, Special issue 06-08 October 2023, Belgrade, Serbia (2023):155-155, https://hdl.handle.net/21.15107/rcub_imagine_2114 .