E-cigarette liquid and condensate leads to impaired embryonic development of zebrafish
Апстракт
Introduction: E-cigarettes are advertised as safer alternative to tradicional cigarettes. However, they
contain chemicals that can exhibit toxic effects on the organism. Notably, effects of e-cigarettes on in
utero development are not well studied. We wanted to compare potential toxic effects of e-cigarette liquid and vapor condensate on development of zebrafish embryos.
Methods: Six hour old zebrafish embryos were exposed to different concentrations of e-cigarette liquid
or vapour condensate – 0.1% and 1%. Untreated embryos were used as control. Each treatment and control were set up in triplicate, with at least 20 embryos per treatment. The effects on survival, hatching and
developmental malformations were monitored using light microscopy, at 3 timepoints - 24, 48 and 72
hours post fertilization (hpf).
Results: No noticeable differences between control and treated groups were observed 24 hpf. Hatched
larvae (35%) treated with 0.1% condensate had scoliosis and malformations- y...olk sac and pericardial
edema at 48 hpf. In groups treated with 1% of condensate or liquid, hatching was delayed and did not
start 48 hpf. At 72 hpf timepoint, in wells with 1% condensate, less than 30% of larvae hatched in total,
which was comparable to wells with e-cigarette liquid (25%). Malformations that were observed in all
treatements are hemagglutionation, pericardial or yolk sac edema, and scoliosis. In groups with 0.1%
condensate these maformations were observed in lower number of embryos, but the percentage of
hatched larvae was higher (approximately 80%) at 72 hpf.
Conclusions: Chronic exposure to e-cigarette vapor condensate and liquid leads to severe disorders of
zebrafish embryonic development.
Кључне речи:
e-cigarettes / toxicology / developmental malformations / zebrafishИзвор:
CoMBoS2 – the Second Congress of Molecular Biologists of Serbia, Abstract Book – Trends in Molecular Biology, Special issue 06-08 October 2023, Belgrade, Serbia, 2023, 73-73Издавач:
- Institute of Molecular Genetics and Genetic Engineering (IMGGE), University of Belgrade
Финансирање / пројекти:
- Министарство науке, технолошког развоја и иновација Републике Србије, институционално финансирање - 200042 (Универзитет у Београду, Институт за молекуларну генетику и генетичко инжењерство) (RS-MESTD-inst-2020-200042)
Колекције
Институција/група
Institut za molekularnu genetiku i genetičko inženjerstvoTY - CONF AU - Ilić, Bojan AU - Ljujić, Mila AU - Trifunović, Sara AU - Divac Rankov, Aleksandra PY - 2023 UR - https://imagine.imgge.bg.ac.rs/handle/123456789/2120 AB - Introduction: E-cigarettes are advertised as safer alternative to tradicional cigarettes. However, they contain chemicals that can exhibit toxic effects on the organism. Notably, effects of e-cigarettes on in utero development are not well studied. We wanted to compare potential toxic effects of e-cigarette liquid and vapor condensate on development of zebrafish embryos. Methods: Six hour old zebrafish embryos were exposed to different concentrations of e-cigarette liquid or vapour condensate – 0.1% and 1%. Untreated embryos were used as control. Each treatment and control were set up in triplicate, with at least 20 embryos per treatment. The effects on survival, hatching and developmental malformations were monitored using light microscopy, at 3 timepoints - 24, 48 and 72 hours post fertilization (hpf). Results: No noticeable differences between control and treated groups were observed 24 hpf. Hatched larvae (35%) treated with 0.1% condensate had scoliosis and malformations- yolk sac and pericardial edema at 48 hpf. In groups treated with 1% of condensate or liquid, hatching was delayed and did not start 48 hpf. At 72 hpf timepoint, in wells with 1% condensate, less than 30% of larvae hatched in total, which was comparable to wells with e-cigarette liquid (25%). Malformations that were observed in all treatements are hemagglutionation, pericardial or yolk sac edema, and scoliosis. In groups with 0.1% condensate these maformations were observed in lower number of embryos, but the percentage of hatched larvae was higher (approximately 80%) at 72 hpf. Conclusions: Chronic exposure to e-cigarette vapor condensate and liquid leads to severe disorders of zebrafish embryonic development. PB - Institute of Molecular Genetics and Genetic Engineering (IMGGE), University of Belgrade C3 - CoMBoS2 – the Second Congress of Molecular Biologists of Serbia, Abstract Book – Trends in Molecular Biology, Special issue 06-08 October 2023, Belgrade, Serbia T1 - E-cigarette liquid and condensate leads to impaired embryonic development of zebrafish EP - 73 SP - 73 UR - https://hdl.handle.net/21.15107/rcub_imagine_2120 ER -
@conference{ author = "Ilić, Bojan and Ljujić, Mila and Trifunović, Sara and Divac Rankov, Aleksandra", year = "2023", abstract = "Introduction: E-cigarettes are advertised as safer alternative to tradicional cigarettes. However, they contain chemicals that can exhibit toxic effects on the organism. Notably, effects of e-cigarettes on in utero development are not well studied. We wanted to compare potential toxic effects of e-cigarette liquid and vapor condensate on development of zebrafish embryos. Methods: Six hour old zebrafish embryos were exposed to different concentrations of e-cigarette liquid or vapour condensate – 0.1% and 1%. Untreated embryos were used as control. Each treatment and control were set up in triplicate, with at least 20 embryos per treatment. The effects on survival, hatching and developmental malformations were monitored using light microscopy, at 3 timepoints - 24, 48 and 72 hours post fertilization (hpf). Results: No noticeable differences between control and treated groups were observed 24 hpf. Hatched larvae (35%) treated with 0.1% condensate had scoliosis and malformations- yolk sac and pericardial edema at 48 hpf. In groups treated with 1% of condensate or liquid, hatching was delayed and did not start 48 hpf. At 72 hpf timepoint, in wells with 1% condensate, less than 30% of larvae hatched in total, which was comparable to wells with e-cigarette liquid (25%). Malformations that were observed in all treatements are hemagglutionation, pericardial or yolk sac edema, and scoliosis. In groups with 0.1% condensate these maformations were observed in lower number of embryos, but the percentage of hatched larvae was higher (approximately 80%) at 72 hpf. Conclusions: Chronic exposure to e-cigarette vapor condensate and liquid leads to severe disorders of zebrafish embryonic development.", publisher = "Institute of Molecular Genetics and Genetic Engineering (IMGGE), University of Belgrade", journal = "CoMBoS2 – the Second Congress of Molecular Biologists of Serbia, Abstract Book – Trends in Molecular Biology, Special issue 06-08 October 2023, Belgrade, Serbia", title = "E-cigarette liquid and condensate leads to impaired embryonic development of zebrafish", pages = "73-73", url = "https://hdl.handle.net/21.15107/rcub_imagine_2120" }
Ilić, B., Ljujić, M., Trifunović, S.,& Divac Rankov, A.. (2023). E-cigarette liquid and condensate leads to impaired embryonic development of zebrafish. in CoMBoS2 – the Second Congress of Molecular Biologists of Serbia, Abstract Book – Trends in Molecular Biology, Special issue 06-08 October 2023, Belgrade, Serbia Institute of Molecular Genetics and Genetic Engineering (IMGGE), University of Belgrade., 73-73. https://hdl.handle.net/21.15107/rcub_imagine_2120
Ilić B, Ljujić M, Trifunović S, Divac Rankov A. E-cigarette liquid and condensate leads to impaired embryonic development of zebrafish. in CoMBoS2 – the Second Congress of Molecular Biologists of Serbia, Abstract Book – Trends in Molecular Biology, Special issue 06-08 October 2023, Belgrade, Serbia. 2023;:73-73. https://hdl.handle.net/21.15107/rcub_imagine_2120 .
Ilić, Bojan, Ljujić, Mila, Trifunović, Sara, Divac Rankov, Aleksandra, "E-cigarette liquid and condensate leads to impaired embryonic development of zebrafish" in CoMBoS2 – the Second Congress of Molecular Biologists of Serbia, Abstract Book – Trends in Molecular Biology, Special issue 06-08 October 2023, Belgrade, Serbia (2023):73-73, https://hdl.handle.net/21.15107/rcub_imagine_2120 .