Short chain fatty acid producing faecalimonas sp. NGB245 isolated from human gut modulates neurosignaling in Caenorhabditis elegans
Аутори
Dinić, MiroslavBisenić, Aleksandar
Jakovljević, Stefan
Nastasijević, Branislav
Brdarić, Emilija
Soković Bajić, Svetlana
Đokić, Jelena
Terzić-Vidojević, Amarela
Golić, Nataša
Конференцијски прилог (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Introduction: Gut-brain axis has been identified as an important target for prevention of neurodegenerative and psychiatric disease. To date, specific microbial strains resident in the intestinal ecosystem
have been described to modulate several behaviour-related functions in the host. Faecalimonas sp. is
anaerobic bacteria affiliated with the family Lachnospiraceae, which represents a highly prevalent beneficial bacteria in the human gut and have potential to be used as next generation probiotic.
Methods: Faecalimonas sp. NGB245 was isolated from human fecal material by pre-inoculation in
BACTEC media followed by serial dilutions spreading on Columbia Blood Agar supplemented with cysteine and sodium thioglycolate in Whitley Anaerobic Workstation. Production of short chain fatty acid
(SCFA) was detected after bacterial growth in Columbia broth supplemented with cellobiose by HPLC.
Host response was followed on Caenorhabditis elegans model by evaluated expression of the genes invo...lved in neurosignaling by qPCR.
Results: We showed that Faecalimonassp. NGB245 exhibits high capacity of production of SCFA including acetate (12,17 mM), propionate (3,02 mM) and butyrate (10,33 mM). Moreover, C. elegansfed with Faecalimonas sp. NGB245 showed higher expression of the genes involved in neurotransmitter synthesis
(tph-1, cat-2), neurotransmitter release (unc-64, snb-1, snt-1), neurotransmitter receptor (npr-1) and different classes of neuropeptides(flp-18, flp-21, nlp-28, nlp-29) in comparison to wormsfed with Escherichia
coli OP50, as a standard laboratory food.
Conclusion: The obtained results imply that Faecalimonas sp. NGB245 isolate could be considered as
next generation probiotic to be used in prevention and treatment of neurodegenerative and psychiatric
diseases.
Кључне речи:
Faecalimonas sp / next generation probiotics / SCFA / Caenorhabditis elegans / neuropeptidesИзвор:
CoMBoS2 – the Second Congress of Molecular Biologists of Serbia, Abstract Book – Trends in Molecular Biology, Special issue 06-08 October 2023, Belgrade, Serbia, 2023, 124-124Издавач:
- Institute of Molecular Genetics and Genetic Engineering (IMGGE), University of Belgrade
Финансирање / пројекти:
- Министарство науке, технолошког развоја и иновација Републике Србије, институционално финансирање - 200042 (Универзитет у Београду, Институт за молекуларну генетику и генетичко инжењерство) (RS-MESTD-inst-2020-200042)
Институција/група
Institut za molekularnu genetiku i genetičko inženjerstvoTY - CONF AU - Dinić, Miroslav AU - Bisenić, Aleksandar AU - Jakovljević, Stefan AU - Nastasijević, Branislav AU - Brdarić, Emilija AU - Soković Bajić, Svetlana AU - Đokić, Jelena AU - Terzić-Vidojević, Amarela AU - Golić, Nataša PY - 2023 UR - https://imagine.imgge.bg.ac.rs/handle/123456789/2129 AB - Introduction: Gut-brain axis has been identified as an important target for prevention of neurodegenerative and psychiatric disease. To date, specific microbial strains resident in the intestinal ecosystem have been described to modulate several behaviour-related functions in the host. Faecalimonas sp. is anaerobic bacteria affiliated with the family Lachnospiraceae, which represents a highly prevalent beneficial bacteria in the human gut and have potential to be used as next generation probiotic. Methods: Faecalimonas sp. NGB245 was isolated from human fecal material by pre-inoculation in BACTEC media followed by serial dilutions spreading on Columbia Blood Agar supplemented with cysteine and sodium thioglycolate in Whitley Anaerobic Workstation. Production of short chain fatty acid (SCFA) was detected after bacterial growth in Columbia broth supplemented with cellobiose by HPLC. Host response was followed on Caenorhabditis elegans model by evaluated expression of the genes involved in neurosignaling by qPCR. Results: We showed that Faecalimonassp. NGB245 exhibits high capacity of production of SCFA including acetate (12,17 mM), propionate (3,02 mM) and butyrate (10,33 mM). Moreover, C. elegansfed with Faecalimonas sp. NGB245 showed higher expression of the genes involved in neurotransmitter synthesis (tph-1, cat-2), neurotransmitter release (unc-64, snb-1, snt-1), neurotransmitter receptor (npr-1) and different classes of neuropeptides(flp-18, flp-21, nlp-28, nlp-29) in comparison to wormsfed with Escherichia coli OP50, as a standard laboratory food. Conclusion: The obtained results imply that Faecalimonas sp. NGB245 isolate could be considered as next generation probiotic to be used in prevention and treatment of neurodegenerative and psychiatric diseases. PB - Institute of Molecular Genetics and Genetic Engineering (IMGGE), University of Belgrade C3 - CoMBoS2 – the Second Congress of Molecular Biologists of Serbia, Abstract Book – Trends in Molecular Biology, Special issue 06-08 October 2023, Belgrade, Serbia T1 - Short chain fatty acid producing faecalimonas sp. NGB245 isolated from human gut modulates neurosignaling in Caenorhabditis elegans EP - 124 SP - 124 UR - https://hdl.handle.net/21.15107/rcub_imagine_2129 ER -
@conference{ author = "Dinić, Miroslav and Bisenić, Aleksandar and Jakovljević, Stefan and Nastasijević, Branislav and Brdarić, Emilija and Soković Bajić, Svetlana and Đokić, Jelena and Terzić-Vidojević, Amarela and Golić, Nataša", year = "2023", abstract = "Introduction: Gut-brain axis has been identified as an important target for prevention of neurodegenerative and psychiatric disease. To date, specific microbial strains resident in the intestinal ecosystem have been described to modulate several behaviour-related functions in the host. Faecalimonas sp. is anaerobic bacteria affiliated with the family Lachnospiraceae, which represents a highly prevalent beneficial bacteria in the human gut and have potential to be used as next generation probiotic. Methods: Faecalimonas sp. NGB245 was isolated from human fecal material by pre-inoculation in BACTEC media followed by serial dilutions spreading on Columbia Blood Agar supplemented with cysteine and sodium thioglycolate in Whitley Anaerobic Workstation. Production of short chain fatty acid (SCFA) was detected after bacterial growth in Columbia broth supplemented with cellobiose by HPLC. Host response was followed on Caenorhabditis elegans model by evaluated expression of the genes involved in neurosignaling by qPCR. Results: We showed that Faecalimonassp. NGB245 exhibits high capacity of production of SCFA including acetate (12,17 mM), propionate (3,02 mM) and butyrate (10,33 mM). Moreover, C. elegansfed with Faecalimonas sp. NGB245 showed higher expression of the genes involved in neurotransmitter synthesis (tph-1, cat-2), neurotransmitter release (unc-64, snb-1, snt-1), neurotransmitter receptor (npr-1) and different classes of neuropeptides(flp-18, flp-21, nlp-28, nlp-29) in comparison to wormsfed with Escherichia coli OP50, as a standard laboratory food. Conclusion: The obtained results imply that Faecalimonas sp. NGB245 isolate could be considered as next generation probiotic to be used in prevention and treatment of neurodegenerative and psychiatric diseases.", publisher = "Institute of Molecular Genetics and Genetic Engineering (IMGGE), University of Belgrade", journal = "CoMBoS2 – the Second Congress of Molecular Biologists of Serbia, Abstract Book – Trends in Molecular Biology, Special issue 06-08 October 2023, Belgrade, Serbia", title = "Short chain fatty acid producing faecalimonas sp. NGB245 isolated from human gut modulates neurosignaling in Caenorhabditis elegans", pages = "124-124", url = "https://hdl.handle.net/21.15107/rcub_imagine_2129" }
Dinić, M., Bisenić, A., Jakovljević, S., Nastasijević, B., Brdarić, E., Soković Bajić, S., Đokić, J., Terzić-Vidojević, A.,& Golić, N.. (2023). Short chain fatty acid producing faecalimonas sp. NGB245 isolated from human gut modulates neurosignaling in Caenorhabditis elegans. in CoMBoS2 – the Second Congress of Molecular Biologists of Serbia, Abstract Book – Trends in Molecular Biology, Special issue 06-08 October 2023, Belgrade, Serbia Institute of Molecular Genetics and Genetic Engineering (IMGGE), University of Belgrade., 124-124. https://hdl.handle.net/21.15107/rcub_imagine_2129
Dinić M, Bisenić A, Jakovljević S, Nastasijević B, Brdarić E, Soković Bajić S, Đokić J, Terzić-Vidojević A, Golić N. Short chain fatty acid producing faecalimonas sp. NGB245 isolated from human gut modulates neurosignaling in Caenorhabditis elegans. in CoMBoS2 – the Second Congress of Molecular Biologists of Serbia, Abstract Book – Trends in Molecular Biology, Special issue 06-08 October 2023, Belgrade, Serbia. 2023;:124-124. https://hdl.handle.net/21.15107/rcub_imagine_2129 .
Dinić, Miroslav, Bisenić, Aleksandar, Jakovljević, Stefan, Nastasijević, Branislav, Brdarić, Emilija, Soković Bajić, Svetlana, Đokić, Jelena, Terzić-Vidojević, Amarela, Golić, Nataša, "Short chain fatty acid producing faecalimonas sp. NGB245 isolated from human gut modulates neurosignaling in Caenorhabditis elegans" in CoMBoS2 – the Second Congress of Molecular Biologists of Serbia, Abstract Book – Trends in Molecular Biology, Special issue 06-08 October 2023, Belgrade, Serbia (2023):124-124, https://hdl.handle.net/21.15107/rcub_imagine_2129 .