Spatial profile of ankrd1a activation during regeneration of zebrafish heart
2023
Authors
Milovanović, MinaBošković, Srđan
Jasnić, Jovana
Novković, Mirjana
Milošević, Emilija
Kojić, Snežana
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Introduction: In contrast to humans, zebrafish have a remarkable ability to regenerate injured heart
through a complex and highly orchestrated processinvolving all cardiac structures. The majorsource of
new myocardial cells are resident cardiomyocytes, which dedifferentiate and reinitiate proliferation, invading the area of injury to replace the lost myocardium. The response of the myocardium and coronary
vasculature is preceded by activation of epi- and endocardium, which form active scaffolds to guide regeneration. The aim of thisstudy wasto identify cardiac structuresin which ankrd1a gene is activated during zebrafish heart regeneration.
Methods: We crossed several zebrafish reporter lines: TgBAC(ankrd1a:EGFP) (to identify cells expressing
ankrd1a), Tg(myl7:nls-dsRedExpress) (for labeling cardiomyocyte nuclei) and Tg(kdrl:RAS-mCherry) (for labeling endocardial/endothelial cells). Zebrafish hearts were cryoinjured and left to regenerate for 3 and
7 days. Dedifferentiating cardi...omyocytes and epicardial cells were immunostained with anti-MYH7 and
anti-caveolin1 antibody, respectively. Cells labeled with transgenes and immunostaining were visualized on tissue cryosections by fluorescent microscopy.
Results: Zebrafish ankrd1a was activated in the injury border zone cardiomyocytes, located between
the injured and remote myocardium. Its expression preceded that of a dedifferentiation marker, MYH7.
The TgBAC(ankrd1a:EGFP) transgene was not detected in epicardial or endocardial cells of regenerating
zebrafish heart.
Conclusion: Activation of ankrd1a during regeneration of zebrafish heart is restricted to borderzone
cardiomyocytes, implicating this gene in dedifferentiation and proliferation of cardiomyocytes. The absence of ankrd1a expression in epicardium and endocardium indicatesthat this gene does not contribute
to the regeneration process occuring in these layers of the heart.
Keywords:
zebrafish / heart / regeneration / ankrd1aSource:
CoMBoS2 – the Second Congress of Molecular Biologists of Serbia, Abstract Book – Trends in Molecular Biology, Special issue 06-08 October 2023, Belgrade, Serbia, 2023, 141-141Publisher:
- Institute of Molecular Genetics and Genetic Engineering (IMGGE), University of Belgrade
Funding / projects:
- ZEBARR - Zebrafish ankrd1a as a common player in heart regeneration and skeletal muscle repair: A new prospect for unlocking regenerative potential of human heart (RS-ScienceFundRS-Ideje-7739807)
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Institution/Community
Institut za molekularnu genetiku i genetičko inženjerstvoTY - CONF AU - Milovanović, Mina AU - Bošković, Srđan AU - Jasnić, Jovana AU - Novković, Mirjana AU - Milošević, Emilija AU - Kojić, Snežana PY - 2023 UR - https://imagine.imgge.bg.ac.rs/handle/123456789/2132 AB - Introduction: In contrast to humans, zebrafish have a remarkable ability to regenerate injured heart through a complex and highly orchestrated processinvolving all cardiac structures. The majorsource of new myocardial cells are resident cardiomyocytes, which dedifferentiate and reinitiate proliferation, invading the area of injury to replace the lost myocardium. The response of the myocardium and coronary vasculature is preceded by activation of epi- and endocardium, which form active scaffolds to guide regeneration. The aim of thisstudy wasto identify cardiac structuresin which ankrd1a gene is activated during zebrafish heart regeneration. Methods: We crossed several zebrafish reporter lines: TgBAC(ankrd1a:EGFP) (to identify cells expressing ankrd1a), Tg(myl7:nls-dsRedExpress) (for labeling cardiomyocyte nuclei) and Tg(kdrl:RAS-mCherry) (for labeling endocardial/endothelial cells). Zebrafish hearts were cryoinjured and left to regenerate for 3 and 7 days. Dedifferentiating cardiomyocytes and epicardial cells were immunostained with anti-MYH7 and anti-caveolin1 antibody, respectively. Cells labeled with transgenes and immunostaining were visualized on tissue cryosections by fluorescent microscopy. Results: Zebrafish ankrd1a was activated in the injury border zone cardiomyocytes, located between the injured and remote myocardium. Its expression preceded that of a dedifferentiation marker, MYH7. The TgBAC(ankrd1a:EGFP) transgene was not detected in epicardial or endocardial cells of regenerating zebrafish heart. Conclusion: Activation of ankrd1a during regeneration of zebrafish heart is restricted to borderzone cardiomyocytes, implicating this gene in dedifferentiation and proliferation of cardiomyocytes. The absence of ankrd1a expression in epicardium and endocardium indicatesthat this gene does not contribute to the regeneration process occuring in these layers of the heart. PB - Institute of Molecular Genetics and Genetic Engineering (IMGGE), University of Belgrade C3 - CoMBoS2 – the Second Congress of Molecular Biologists of Serbia, Abstract Book – Trends in Molecular Biology, Special issue 06-08 October 2023, Belgrade, Serbia T1 - Spatial profile of ankrd1a activation during regeneration of zebrafish heart EP - 141 SP - 141 UR - https://hdl.handle.net/21.15107/rcub_imagine_2132 ER -
@conference{ author = "Milovanović, Mina and Bošković, Srđan and Jasnić, Jovana and Novković, Mirjana and Milošević, Emilija and Kojić, Snežana", year = "2023", abstract = "Introduction: In contrast to humans, zebrafish have a remarkable ability to regenerate injured heart through a complex and highly orchestrated processinvolving all cardiac structures. The majorsource of new myocardial cells are resident cardiomyocytes, which dedifferentiate and reinitiate proliferation, invading the area of injury to replace the lost myocardium. The response of the myocardium and coronary vasculature is preceded by activation of epi- and endocardium, which form active scaffolds to guide regeneration. The aim of thisstudy wasto identify cardiac structuresin which ankrd1a gene is activated during zebrafish heart regeneration. Methods: We crossed several zebrafish reporter lines: TgBAC(ankrd1a:EGFP) (to identify cells expressing ankrd1a), Tg(myl7:nls-dsRedExpress) (for labeling cardiomyocyte nuclei) and Tg(kdrl:RAS-mCherry) (for labeling endocardial/endothelial cells). Zebrafish hearts were cryoinjured and left to regenerate for 3 and 7 days. Dedifferentiating cardiomyocytes and epicardial cells were immunostained with anti-MYH7 and anti-caveolin1 antibody, respectively. Cells labeled with transgenes and immunostaining were visualized on tissue cryosections by fluorescent microscopy. Results: Zebrafish ankrd1a was activated in the injury border zone cardiomyocytes, located between the injured and remote myocardium. Its expression preceded that of a dedifferentiation marker, MYH7. The TgBAC(ankrd1a:EGFP) transgene was not detected in epicardial or endocardial cells of regenerating zebrafish heart. Conclusion: Activation of ankrd1a during regeneration of zebrafish heart is restricted to borderzone cardiomyocytes, implicating this gene in dedifferentiation and proliferation of cardiomyocytes. The absence of ankrd1a expression in epicardium and endocardium indicatesthat this gene does not contribute to the regeneration process occuring in these layers of the heart.", publisher = "Institute of Molecular Genetics and Genetic Engineering (IMGGE), University of Belgrade", journal = "CoMBoS2 – the Second Congress of Molecular Biologists of Serbia, Abstract Book – Trends in Molecular Biology, Special issue 06-08 October 2023, Belgrade, Serbia", title = "Spatial profile of ankrd1a activation during regeneration of zebrafish heart", pages = "141-141", url = "https://hdl.handle.net/21.15107/rcub_imagine_2132" }
Milovanović, M., Bošković, S., Jasnić, J., Novković, M., Milošević, E.,& Kojić, S.. (2023). Spatial profile of ankrd1a activation during regeneration of zebrafish heart. in CoMBoS2 – the Second Congress of Molecular Biologists of Serbia, Abstract Book – Trends in Molecular Biology, Special issue 06-08 October 2023, Belgrade, Serbia Institute of Molecular Genetics and Genetic Engineering (IMGGE), University of Belgrade., 141-141. https://hdl.handle.net/21.15107/rcub_imagine_2132
Milovanović M, Bošković S, Jasnić J, Novković M, Milošević E, Kojić S. Spatial profile of ankrd1a activation during regeneration of zebrafish heart. in CoMBoS2 – the Second Congress of Molecular Biologists of Serbia, Abstract Book – Trends in Molecular Biology, Special issue 06-08 October 2023, Belgrade, Serbia. 2023;:141-141. https://hdl.handle.net/21.15107/rcub_imagine_2132 .
Milovanović, Mina, Bošković, Srđan, Jasnić, Jovana, Novković, Mirjana, Milošević, Emilija, Kojić, Snežana, "Spatial profile of ankrd1a activation during regeneration of zebrafish heart" in CoMBoS2 – the Second Congress of Molecular Biologists of Serbia, Abstract Book – Trends in Molecular Biology, Special issue 06-08 October 2023, Belgrade, Serbia (2023):141-141, https://hdl.handle.net/21.15107/rcub_imagine_2132 .