Unveiling therapeutic potential of bacteriophage treatment in acinetobacter baumannii-infected zebrafish embryo model
Апстракт
Introduction: There is an urgent demand for the development of new therapeutic approachesto combat multidrug-resistant Acinetobacter baumannii, and bacteriophages appear to be a highly promising
solution. Phages are suitable to precisely target the infection-causing bacteria without disrupting the
beneficial microbiota. The zebrafish (Danio rerio) embryo model represents an insightful animal model
for preclinical studying of various infectious diseases and for discovery of novel safe and effective antimicrobial drugs.
Methods: Systemic bacterial infection was established by microinjection of 2000 cells of nosocomial
carbapenem-resistant A. baumannii strain 6077/12 into the bloodstream of 48 hour old zebrafish embryos. Infected embryos were treated by parenteral administration of 4 different doses (10, 50, 100, 500
PFU) of bacteriophage vB_AbaM_ISTD at 6 hours after infection (hpi). Efficacy of treatment was evaluated according to embryo survival, morphological malformations and b...acterial burden (CFU) over a 3-
day period.
Results: A. baumannii-infected embryos treated with bacteriophage resulted with 100% survival rate,
while 70% of untreated embryos survived to 24 hpi and none to the end of the experiment. Viable bacterial cell count and embryo morphology observations indicated that the administered phage effectively reduced A. baumanii infection in vivo. The most effective dose was 500 PFU, decreasing the bacterial
load by 3.09 log units during 24 hpi, while lower bacteriophage doses(10, 50 and 100 PFU) produced less
prominent, but also significant bacterial reduction of 2.10, 2.19 and 2.67 log units, respectively.
Conclusion: Parentheral administration of phage ISTD demonstrated potent therapeutic activity against
A. baumannii infection in every investigated dose.
Кључне речи:
bacteriophage / Acinetobacter / zebrafish / therapy / antimicrobialИзвор:
CoMBoS2 – the Second Congress of Molecular Biologists of Serbia, Abstract Book – Trends in Molecular Biology, Special issue 06-08 October 2023, Belgrade, Serbia, 2023, 83-83Издавач:
- Institute of Molecular Genetics and Genetic Engineering (IMGGE), University of Belgrade
Финансирање / пројекти:
- Министарство науке, технолошког развоја и иновација Републике Србије, институционално финансирање - 200042 (Универзитет у Београду, Институт за молекуларну генетику и генетичко инжењерство) (RS-MESTD-inst-2020-200042)
Колекције
Институција/група
Institut za molekularnu genetiku i genetičko inženjerstvoTY - CONF AU - Obradović, Mina AU - Pavić, Aleksandra AU - Vukotić, Goran PY - 2023 UR - https://imagine.imgge.bg.ac.rs/handle/123456789/2137 AB - Introduction: There is an urgent demand for the development of new therapeutic approachesto combat multidrug-resistant Acinetobacter baumannii, and bacteriophages appear to be a highly promising solution. Phages are suitable to precisely target the infection-causing bacteria without disrupting the beneficial microbiota. The zebrafish (Danio rerio) embryo model represents an insightful animal model for preclinical studying of various infectious diseases and for discovery of novel safe and effective antimicrobial drugs. Methods: Systemic bacterial infection was established by microinjection of 2000 cells of nosocomial carbapenem-resistant A. baumannii strain 6077/12 into the bloodstream of 48 hour old zebrafish embryos. Infected embryos were treated by parenteral administration of 4 different doses (10, 50, 100, 500 PFU) of bacteriophage vB_AbaM_ISTD at 6 hours after infection (hpi). Efficacy of treatment was evaluated according to embryo survival, morphological malformations and bacterial burden (CFU) over a 3- day period. Results: A. baumannii-infected embryos treated with bacteriophage resulted with 100% survival rate, while 70% of untreated embryos survived to 24 hpi and none to the end of the experiment. Viable bacterial cell count and embryo morphology observations indicated that the administered phage effectively reduced A. baumanii infection in vivo. The most effective dose was 500 PFU, decreasing the bacterial load by 3.09 log units during 24 hpi, while lower bacteriophage doses(10, 50 and 100 PFU) produced less prominent, but also significant bacterial reduction of 2.10, 2.19 and 2.67 log units, respectively. Conclusion: Parentheral administration of phage ISTD demonstrated potent therapeutic activity against A. baumannii infection in every investigated dose. PB - Institute of Molecular Genetics and Genetic Engineering (IMGGE), University of Belgrade C3 - CoMBoS2 – the Second Congress of Molecular Biologists of Serbia, Abstract Book – Trends in Molecular Biology, Special issue 06-08 October 2023, Belgrade, Serbia T1 - Unveiling therapeutic potential of bacteriophage treatment in acinetobacter baumannii-infected zebrafish embryo model EP - 83 SP - 83 UR - https://hdl.handle.net/21.15107/rcub_imagine_2137 ER -
@conference{ author = "Obradović, Mina and Pavić, Aleksandra and Vukotić, Goran", year = "2023", abstract = "Introduction: There is an urgent demand for the development of new therapeutic approachesto combat multidrug-resistant Acinetobacter baumannii, and bacteriophages appear to be a highly promising solution. Phages are suitable to precisely target the infection-causing bacteria without disrupting the beneficial microbiota. The zebrafish (Danio rerio) embryo model represents an insightful animal model for preclinical studying of various infectious diseases and for discovery of novel safe and effective antimicrobial drugs. Methods: Systemic bacterial infection was established by microinjection of 2000 cells of nosocomial carbapenem-resistant A. baumannii strain 6077/12 into the bloodstream of 48 hour old zebrafish embryos. Infected embryos were treated by parenteral administration of 4 different doses (10, 50, 100, 500 PFU) of bacteriophage vB_AbaM_ISTD at 6 hours after infection (hpi). Efficacy of treatment was evaluated according to embryo survival, morphological malformations and bacterial burden (CFU) over a 3- day period. Results: A. baumannii-infected embryos treated with bacteriophage resulted with 100% survival rate, while 70% of untreated embryos survived to 24 hpi and none to the end of the experiment. Viable bacterial cell count and embryo morphology observations indicated that the administered phage effectively reduced A. baumanii infection in vivo. The most effective dose was 500 PFU, decreasing the bacterial load by 3.09 log units during 24 hpi, while lower bacteriophage doses(10, 50 and 100 PFU) produced less prominent, but also significant bacterial reduction of 2.10, 2.19 and 2.67 log units, respectively. Conclusion: Parentheral administration of phage ISTD demonstrated potent therapeutic activity against A. baumannii infection in every investigated dose.", publisher = "Institute of Molecular Genetics and Genetic Engineering (IMGGE), University of Belgrade", journal = "CoMBoS2 – the Second Congress of Molecular Biologists of Serbia, Abstract Book – Trends in Molecular Biology, Special issue 06-08 October 2023, Belgrade, Serbia", title = "Unveiling therapeutic potential of bacteriophage treatment in acinetobacter baumannii-infected zebrafish embryo model", pages = "83-83", url = "https://hdl.handle.net/21.15107/rcub_imagine_2137" }
Obradović, M., Pavić, A.,& Vukotić, G.. (2023). Unveiling therapeutic potential of bacteriophage treatment in acinetobacter baumannii-infected zebrafish embryo model. in CoMBoS2 – the Second Congress of Molecular Biologists of Serbia, Abstract Book – Trends in Molecular Biology, Special issue 06-08 October 2023, Belgrade, Serbia Institute of Molecular Genetics and Genetic Engineering (IMGGE), University of Belgrade., 83-83. https://hdl.handle.net/21.15107/rcub_imagine_2137
Obradović M, Pavić A, Vukotić G. Unveiling therapeutic potential of bacteriophage treatment in acinetobacter baumannii-infected zebrafish embryo model. in CoMBoS2 – the Second Congress of Molecular Biologists of Serbia, Abstract Book – Trends in Molecular Biology, Special issue 06-08 October 2023, Belgrade, Serbia. 2023;:83-83. https://hdl.handle.net/21.15107/rcub_imagine_2137 .
Obradović, Mina, Pavić, Aleksandra, Vukotić, Goran, "Unveiling therapeutic potential of bacteriophage treatment in acinetobacter baumannii-infected zebrafish embryo model" in CoMBoS2 – the Second Congress of Molecular Biologists of Serbia, Abstract Book – Trends in Molecular Biology, Special issue 06-08 October 2023, Belgrade, Serbia (2023):83-83, https://hdl.handle.net/21.15107/rcub_imagine_2137 .