Bacterial nanocellulose – new beginning for end-of-life plastics
Апстракт
Introduction: Fossil-based polymers continue to be widely used despite their negative environmental
impact. Bioplastics, such as polylactic acid (PLA), offer a promising alternative as they are derived from
renewable resources and provide more environmentally friendly end-of-life options. However, marketing PLA assimply biodegradable can be misleading, asthe current PLA degradation strategy contributes
to microplastics pollution, thus posing even greater threat. Thisresearch focuses on the upcycling of PLA
degradation products into valuable biomaterial - bacterial nanocellulose.
Methods: PLA samples were pretreated using ultraviolet and ultrasonic waves, individually and in combination, to enhancessusceptibility to bacterial degradation. Pretreated PLA wassubjected to enzymatic
degradation under mild conditions, using various enzyme combinations. The resulting biodegradation
products served as a growth medium for nanocellulose producing bacteria Komagataeibacter medellinensis ID...13488. Obtained nanocellulose was characterized using SEM, FTIR, AFM, and XRD.
Results: The combined PLA pretreatment using ultraviolet and ultrasonic waves, followed by enzymatic
degradation with savinase, demonstrated the highest degree of PLA degradation in this study. Furthermore, K. medellinensis ID13488 efficiently utilized the biodegradation products, producing nanocellulose with yields and performance comparable to those obtained through standard cultivation using
glucose as a carbon source.
Conclusion: This study highlights the potential of combined pretreatment and enzymatic degradation
for efficient PLA degradation and sustainable bacterial nanocellulose production. The findings suggest
promising avenues for utilizing PLA biodegradation products in the production of other valuable biomaterials. Further research is needed to optimize the pretreatment and degradation processes, facilitating the wider application of biodegradable materials and promoting sustainability.
Кључне речи:
PLA / pretreatment / biodegradation / savinase / bacterial nanocelluloseИзвор:
CoMBoS2 – the Second Congress of Molecular Biologists of Serbia, Abstract Book – Trends in Molecular Biology, Special issue 06-08 October 2023, Belgrade, Serbia, 2023, 107-107Издавач:
- Institute of Molecular Genetics and Genetic Engineering (IMGGE), University of Belgrade
Финансирање / пројекти:
- Министарство науке, технолошког развоја и иновација Републике Србије, институционално финансирање - 200042 (Универзитет у Београду, Институт за молекуларну генетику и генетичко инжењерство) (RS-MESTD-inst-2020-200042)
- Horizon 2020 program - BioICEP project (Agreement no. 870292)
- Horizon Europe program - EcoPlastiC project (Agreement no. 101046758)
Институција/група
Institut za molekularnu genetiku i genetičko inženjerstvoTY - CONF AU - Jeremić, Sanja PY - 2023 UR - https://imagine.imgge.bg.ac.rs/handle/123456789/2144 AB - Introduction: Fossil-based polymers continue to be widely used despite their negative environmental impact. Bioplastics, such as polylactic acid (PLA), offer a promising alternative as they are derived from renewable resources and provide more environmentally friendly end-of-life options. However, marketing PLA assimply biodegradable can be misleading, asthe current PLA degradation strategy contributes to microplastics pollution, thus posing even greater threat. Thisresearch focuses on the upcycling of PLA degradation products into valuable biomaterial - bacterial nanocellulose. Methods: PLA samples were pretreated using ultraviolet and ultrasonic waves, individually and in combination, to enhancessusceptibility to bacterial degradation. Pretreated PLA wassubjected to enzymatic degradation under mild conditions, using various enzyme combinations. The resulting biodegradation products served as a growth medium for nanocellulose producing bacteria Komagataeibacter medellinensis ID13488. Obtained nanocellulose was characterized using SEM, FTIR, AFM, and XRD. Results: The combined PLA pretreatment using ultraviolet and ultrasonic waves, followed by enzymatic degradation with savinase, demonstrated the highest degree of PLA degradation in this study. Furthermore, K. medellinensis ID13488 efficiently utilized the biodegradation products, producing nanocellulose with yields and performance comparable to those obtained through standard cultivation using glucose as a carbon source. Conclusion: This study highlights the potential of combined pretreatment and enzymatic degradation for efficient PLA degradation and sustainable bacterial nanocellulose production. The findings suggest promising avenues for utilizing PLA biodegradation products in the production of other valuable biomaterials. Further research is needed to optimize the pretreatment and degradation processes, facilitating the wider application of biodegradable materials and promoting sustainability. PB - Institute of Molecular Genetics and Genetic Engineering (IMGGE), University of Belgrade C3 - CoMBoS2 – the Second Congress of Molecular Biologists of Serbia, Abstract Book – Trends in Molecular Biology, Special issue 06-08 October 2023, Belgrade, Serbia T1 - Bacterial nanocellulose – new beginning for end-of-life plastics EP - 107 SP - 107 UR - https://hdl.handle.net/21.15107/rcub_imagine_2144 ER -
@conference{ author = "Jeremić, Sanja", year = "2023", abstract = "Introduction: Fossil-based polymers continue to be widely used despite their negative environmental impact. Bioplastics, such as polylactic acid (PLA), offer a promising alternative as they are derived from renewable resources and provide more environmentally friendly end-of-life options. However, marketing PLA assimply biodegradable can be misleading, asthe current PLA degradation strategy contributes to microplastics pollution, thus posing even greater threat. Thisresearch focuses on the upcycling of PLA degradation products into valuable biomaterial - bacterial nanocellulose. Methods: PLA samples were pretreated using ultraviolet and ultrasonic waves, individually and in combination, to enhancessusceptibility to bacterial degradation. Pretreated PLA wassubjected to enzymatic degradation under mild conditions, using various enzyme combinations. The resulting biodegradation products served as a growth medium for nanocellulose producing bacteria Komagataeibacter medellinensis ID13488. Obtained nanocellulose was characterized using SEM, FTIR, AFM, and XRD. Results: The combined PLA pretreatment using ultraviolet and ultrasonic waves, followed by enzymatic degradation with savinase, demonstrated the highest degree of PLA degradation in this study. Furthermore, K. medellinensis ID13488 efficiently utilized the biodegradation products, producing nanocellulose with yields and performance comparable to those obtained through standard cultivation using glucose as a carbon source. Conclusion: This study highlights the potential of combined pretreatment and enzymatic degradation for efficient PLA degradation and sustainable bacterial nanocellulose production. The findings suggest promising avenues for utilizing PLA biodegradation products in the production of other valuable biomaterials. Further research is needed to optimize the pretreatment and degradation processes, facilitating the wider application of biodegradable materials and promoting sustainability.", publisher = "Institute of Molecular Genetics and Genetic Engineering (IMGGE), University of Belgrade", journal = "CoMBoS2 – the Second Congress of Molecular Biologists of Serbia, Abstract Book – Trends in Molecular Biology, Special issue 06-08 October 2023, Belgrade, Serbia", title = "Bacterial nanocellulose – new beginning for end-of-life plastics", pages = "107-107", url = "https://hdl.handle.net/21.15107/rcub_imagine_2144" }
Jeremić, S.. (2023). Bacterial nanocellulose – new beginning for end-of-life plastics. in CoMBoS2 – the Second Congress of Molecular Biologists of Serbia, Abstract Book – Trends in Molecular Biology, Special issue 06-08 October 2023, Belgrade, Serbia Institute of Molecular Genetics and Genetic Engineering (IMGGE), University of Belgrade., 107-107. https://hdl.handle.net/21.15107/rcub_imagine_2144
Jeremić S. Bacterial nanocellulose – new beginning for end-of-life plastics. in CoMBoS2 – the Second Congress of Molecular Biologists of Serbia, Abstract Book – Trends in Molecular Biology, Special issue 06-08 October 2023, Belgrade, Serbia. 2023;:107-107. https://hdl.handle.net/21.15107/rcub_imagine_2144 .
Jeremić, Sanja, "Bacterial nanocellulose – new beginning for end-of-life plastics" in CoMBoS2 – the Second Congress of Molecular Biologists of Serbia, Abstract Book – Trends in Molecular Biology, Special issue 06-08 October 2023, Belgrade, Serbia (2023):107-107, https://hdl.handle.net/21.15107/rcub_imagine_2144 .