The role of the gut bacteria during host aging
Аутори
Dinić, MiroslavJakovljević, Stefan
Radojević, Dušan
Brdarić, Emilija
Soković Bajić, Svetlana
Đokić, Jelena
Golić, Nataša
Конференцијски прилог (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Introduction: Microbial community established in the gut has been recognized as an important factor
which influence host aging. Bacteria from the gut co-evolved with the host resulting in mutually beneficial interactions essential for host’s wellbeing. This complex crosstalk reflects mainly through the interaction between bacterial macromolecules (e.g., exopolysaccharides) and the host receptors leading
to the activation of various cellular pathways. Here, we explore the potential of different lactobacilli,
commonly used as probiotics, to activate longevity signalling in Caenorhabditis elegans.
Methods: Evaluation of C. elegans lifespan and aging parameters (locomotion rate and pharyngeal
pumping) were performed by feeding N2 wild-type worms with different Lactobacillus species. Worms
fed with selected strains were subjected to RNAseq analysis, qPCR and Western blot to evaluate activation of autophagy, immunity, antioxidative response and mitochondrial function. Activation of aut...ophagy was confirmed in DA2123 GFP-labelled LGG-1 transgenic strain and JIN1375 hlh-30 (tm1978)
mutant, while immunity activation was evaluated by using KU25 pmk-1 (km25) mutant and through
nematode killing assays.
Results: Selected strains of lactobacilli promoted health and lifespan of worms through activation of
TFEB/HLH-30 dependent autophagy and p38 MAPK/PMK-1 dependent immune response which provided resistance of worms exposed to pathogens. Moreover, RNAseq analysis identified core gene signature associate with exopolysaccharide-induced longevity highlighting involvement of fmo-2, gsto-1,
nlp-29, and clec-47 genes in increased lifespan of the worms.
Conclusion: Analyzed lactobacilli showed potential to promote healthy aging and could be further investigated in order to better understand application of lactobacilli as pro-longevity probiotics.
Кључне речи:
Lactobacillus / Caenorhabditis elegans / autophagy / immunity / agingИзвор:
CoMBoS2 – the Second Congress of Molecular Biologists of Serbia, Abstract Book – Trends in Molecular Biology, Special issue 06-08 October 2023, Belgrade, Serbia, 2023, 108-108Издавач:
- Institute of Molecular Genetics and Genetic Engineering (IMGGE), University of Belgrade
Финансирање / пројекти:
- Министарство науке, технолошког развоја и иновација Републике Србије, институционално финансирање - 200042 (Универзитет у Београду, Институт за молекуларну генетику и генетичко инжењерство) (RS-MESTD-inst-2020-200042)
Институција/група
Institut za molekularnu genetiku i genetičko inženjerstvoTY - CONF AU - Dinić, Miroslav AU - Jakovljević, Stefan AU - Radojević, Dušan AU - Brdarić, Emilija AU - Soković Bajić, Svetlana AU - Đokić, Jelena AU - Golić, Nataša PY - 2023 UR - https://imagine.imgge.bg.ac.rs/handle/123456789/2146 AB - Introduction: Microbial community established in the gut has been recognized as an important factor which influence host aging. Bacteria from the gut co-evolved with the host resulting in mutually beneficial interactions essential for host’s wellbeing. This complex crosstalk reflects mainly through the interaction between bacterial macromolecules (e.g., exopolysaccharides) and the host receptors leading to the activation of various cellular pathways. Here, we explore the potential of different lactobacilli, commonly used as probiotics, to activate longevity signalling in Caenorhabditis elegans. Methods: Evaluation of C. elegans lifespan and aging parameters (locomotion rate and pharyngeal pumping) were performed by feeding N2 wild-type worms with different Lactobacillus species. Worms fed with selected strains were subjected to RNAseq analysis, qPCR and Western blot to evaluate activation of autophagy, immunity, antioxidative response and mitochondrial function. Activation of autophagy was confirmed in DA2123 GFP-labelled LGG-1 transgenic strain and JIN1375 hlh-30 (tm1978) mutant, while immunity activation was evaluated by using KU25 pmk-1 (km25) mutant and through nematode killing assays. Results: Selected strains of lactobacilli promoted health and lifespan of worms through activation of TFEB/HLH-30 dependent autophagy and p38 MAPK/PMK-1 dependent immune response which provided resistance of worms exposed to pathogens. Moreover, RNAseq analysis identified core gene signature associate with exopolysaccharide-induced longevity highlighting involvement of fmo-2, gsto-1, nlp-29, and clec-47 genes in increased lifespan of the worms. Conclusion: Analyzed lactobacilli showed potential to promote healthy aging and could be further investigated in order to better understand application of lactobacilli as pro-longevity probiotics. PB - Institute of Molecular Genetics and Genetic Engineering (IMGGE), University of Belgrade C3 - CoMBoS2 – the Second Congress of Molecular Biologists of Serbia, Abstract Book – Trends in Molecular Biology, Special issue 06-08 October 2023, Belgrade, Serbia T1 - The role of the gut bacteria during host aging EP - 108 SP - 108 UR - https://hdl.handle.net/21.15107/rcub_imagine_2146 ER -
@conference{ author = "Dinić, Miroslav and Jakovljević, Stefan and Radojević, Dušan and Brdarić, Emilija and Soković Bajić, Svetlana and Đokić, Jelena and Golić, Nataša", year = "2023", abstract = "Introduction: Microbial community established in the gut has been recognized as an important factor which influence host aging. Bacteria from the gut co-evolved with the host resulting in mutually beneficial interactions essential for host’s wellbeing. This complex crosstalk reflects mainly through the interaction between bacterial macromolecules (e.g., exopolysaccharides) and the host receptors leading to the activation of various cellular pathways. Here, we explore the potential of different lactobacilli, commonly used as probiotics, to activate longevity signalling in Caenorhabditis elegans. Methods: Evaluation of C. elegans lifespan and aging parameters (locomotion rate and pharyngeal pumping) were performed by feeding N2 wild-type worms with different Lactobacillus species. Worms fed with selected strains were subjected to RNAseq analysis, qPCR and Western blot to evaluate activation of autophagy, immunity, antioxidative response and mitochondrial function. Activation of autophagy was confirmed in DA2123 GFP-labelled LGG-1 transgenic strain and JIN1375 hlh-30 (tm1978) mutant, while immunity activation was evaluated by using KU25 pmk-1 (km25) mutant and through nematode killing assays. Results: Selected strains of lactobacilli promoted health and lifespan of worms through activation of TFEB/HLH-30 dependent autophagy and p38 MAPK/PMK-1 dependent immune response which provided resistance of worms exposed to pathogens. Moreover, RNAseq analysis identified core gene signature associate with exopolysaccharide-induced longevity highlighting involvement of fmo-2, gsto-1, nlp-29, and clec-47 genes in increased lifespan of the worms. Conclusion: Analyzed lactobacilli showed potential to promote healthy aging and could be further investigated in order to better understand application of lactobacilli as pro-longevity probiotics.", publisher = "Institute of Molecular Genetics and Genetic Engineering (IMGGE), University of Belgrade", journal = "CoMBoS2 – the Second Congress of Molecular Biologists of Serbia, Abstract Book – Trends in Molecular Biology, Special issue 06-08 October 2023, Belgrade, Serbia", title = "The role of the gut bacteria during host aging", pages = "108-108", url = "https://hdl.handle.net/21.15107/rcub_imagine_2146" }
Dinić, M., Jakovljević, S., Radojević, D., Brdarić, E., Soković Bajić, S., Đokić, J.,& Golić, N.. (2023). The role of the gut bacteria during host aging. in CoMBoS2 – the Second Congress of Molecular Biologists of Serbia, Abstract Book – Trends in Molecular Biology, Special issue 06-08 October 2023, Belgrade, Serbia Institute of Molecular Genetics and Genetic Engineering (IMGGE), University of Belgrade., 108-108. https://hdl.handle.net/21.15107/rcub_imagine_2146
Dinić M, Jakovljević S, Radojević D, Brdarić E, Soković Bajić S, Đokić J, Golić N. The role of the gut bacteria during host aging. in CoMBoS2 – the Second Congress of Molecular Biologists of Serbia, Abstract Book – Trends in Molecular Biology, Special issue 06-08 October 2023, Belgrade, Serbia. 2023;:108-108. https://hdl.handle.net/21.15107/rcub_imagine_2146 .
Dinić, Miroslav, Jakovljević, Stefan, Radojević, Dušan, Brdarić, Emilija, Soković Bajić, Svetlana, Đokić, Jelena, Golić, Nataša, "The role of the gut bacteria during host aging" in CoMBoS2 – the Second Congress of Molecular Biologists of Serbia, Abstract Book – Trends in Molecular Biology, Special issue 06-08 October 2023, Belgrade, Serbia (2023):108-108, https://hdl.handle.net/21.15107/rcub_imagine_2146 .