CONSUMPTION OF EXOPOLYSACCHARIDES FROM LACTIPLANTIBACILLUS PLANTARUM BGAN8 ALTERS THE GUT MICROBIOTA OF DA RAT
Autori
Brdarić, EmilijaSoković Bajić, Svetlana
Popović, Dušanka
Kulaš, Jelena
Popov Aleksandrov, Aleksandra
Mirkov, Ivana
Živković, Milica
Konferencijski prilog (Objavljena verzija)
Metapodaci
Prikaz svih podataka o dokumentuApstrakt
Introduction: Exopolysaccharides (EPS) are carbohydrate polymers with important biological activities such
as immunomodulatory, antioxidative and antitumor (1). How EPS affect the gut microbiota, which plays an
important role in maintaining homeostasis in the organism, is poorly understood to date. Therefore, the aim
of this study was to investigate how EPS-AN8, derived from Lactiplantibacillus plantarum BGAN8, affects part
of the gut microbiota in the duodenum of healthy Dark Agouti rats (DA).
Materials and Methods: EPS-AN8 was administered to male DA at low (0.1 mg/mL) and high (1 mg/mL)
dose over a 15-day period. Total duodenal DNA was isolated and PCR amplicon for 16SrRNA was sequenced
on Illumina NovaSeq paired-end platform. Furthermore, we tracked key parameters of oxidative stress and
inflammation in duodenal homogenates.
Results: The higher dose of EPS-AN8 resulted in an increased Shannon's diversity index. The most
significant differences were observed in the... increased relative abundance of the genera Ruminococcus,
Dubosiella, Enterohabdus, and Adlercreutzia. At the same time, we demonstrated that neither dosage caused
negative side effects such as inflammation and oxidative stress.
Conclusion: Considering the existing trend to market foods with additional health benefits, our results
suggest that EPS-AN8 could be a good candidate for functional food supplementation.
Izvor:
10th ISM World Congress on Targeting Microbiota, 2023, 61-61Finansiranje / projekti:
- Ministarstvo nauke, tehnološkog razvoja i inovacija Republike Srbije, institucionalno finansiranje - 200007 (Univerzitet u Beogradu, Institut za biološka istraživanja 'Siniša Stanković') (RS-MESTD-inst-2020-200007)
Napomena:
- International Society of Microbiota 10th ISM World Congress on Targeting Microbiota October 17-19, 2023 – Venice, Italy
Institucija/grupa
Institut za molekularnu genetiku i genetičko inženjerstvoTY - CONF AU - Brdarić, Emilija AU - Soković Bajić, Svetlana AU - Popović, Dušanka AU - Kulaš, Jelena AU - Popov Aleksandrov, Aleksandra AU - Mirkov, Ivana AU - Živković, Milica PY - 2023 UR - https://www.microbiota-site.com/ UR - https://imagine.imgge.bg.ac.rs/handle/123456789/2186 AB - Introduction: Exopolysaccharides (EPS) are carbohydrate polymers with important biological activities such as immunomodulatory, antioxidative and antitumor (1). How EPS affect the gut microbiota, which plays an important role in maintaining homeostasis in the organism, is poorly understood to date. Therefore, the aim of this study was to investigate how EPS-AN8, derived from Lactiplantibacillus plantarum BGAN8, affects part of the gut microbiota in the duodenum of healthy Dark Agouti rats (DA). Materials and Methods: EPS-AN8 was administered to male DA at low (0.1 mg/mL) and high (1 mg/mL) dose over a 15-day period. Total duodenal DNA was isolated and PCR amplicon for 16SrRNA was sequenced on Illumina NovaSeq paired-end platform. Furthermore, we tracked key parameters of oxidative stress and inflammation in duodenal homogenates. Results: The higher dose of EPS-AN8 resulted in an increased Shannon's diversity index. The most significant differences were observed in the increased relative abundance of the genera Ruminococcus, Dubosiella, Enterohabdus, and Adlercreutzia. At the same time, we demonstrated that neither dosage caused negative side effects such as inflammation and oxidative stress. Conclusion: Considering the existing trend to market foods with additional health benefits, our results suggest that EPS-AN8 could be a good candidate for functional food supplementation. C3 - 10th ISM World Congress on Targeting Microbiota T1 - CONSUMPTION OF EXOPOLYSACCHARIDES FROM LACTIPLANTIBACILLUS PLANTARUM BGAN8 ALTERS THE GUT MICROBIOTA OF DA RAT EP - 61 SP - 61 UR - https://hdl.handle.net/21.15107/rcub_imagine_2186 ER -
@conference{ author = "Brdarić, Emilija and Soković Bajić, Svetlana and Popović, Dušanka and Kulaš, Jelena and Popov Aleksandrov, Aleksandra and Mirkov, Ivana and Živković, Milica", year = "2023", abstract = "Introduction: Exopolysaccharides (EPS) are carbohydrate polymers with important biological activities such as immunomodulatory, antioxidative and antitumor (1). How EPS affect the gut microbiota, which plays an important role in maintaining homeostasis in the organism, is poorly understood to date. Therefore, the aim of this study was to investigate how EPS-AN8, derived from Lactiplantibacillus plantarum BGAN8, affects part of the gut microbiota in the duodenum of healthy Dark Agouti rats (DA). Materials and Methods: EPS-AN8 was administered to male DA at low (0.1 mg/mL) and high (1 mg/mL) dose over a 15-day period. Total duodenal DNA was isolated and PCR amplicon for 16SrRNA was sequenced on Illumina NovaSeq paired-end platform. Furthermore, we tracked key parameters of oxidative stress and inflammation in duodenal homogenates. Results: The higher dose of EPS-AN8 resulted in an increased Shannon's diversity index. The most significant differences were observed in the increased relative abundance of the genera Ruminococcus, Dubosiella, Enterohabdus, and Adlercreutzia. At the same time, we demonstrated that neither dosage caused negative side effects such as inflammation and oxidative stress. Conclusion: Considering the existing trend to market foods with additional health benefits, our results suggest that EPS-AN8 could be a good candidate for functional food supplementation.", journal = "10th ISM World Congress on Targeting Microbiota", title = "CONSUMPTION OF EXOPOLYSACCHARIDES FROM LACTIPLANTIBACILLUS PLANTARUM BGAN8 ALTERS THE GUT MICROBIOTA OF DA RAT", pages = "61-61", url = "https://hdl.handle.net/21.15107/rcub_imagine_2186" }
Brdarić, E., Soković Bajić, S., Popović, D., Kulaš, J., Popov Aleksandrov, A., Mirkov, I.,& Živković, M.. (2023). CONSUMPTION OF EXOPOLYSACCHARIDES FROM LACTIPLANTIBACILLUS PLANTARUM BGAN8 ALTERS THE GUT MICROBIOTA OF DA RAT. in 10th ISM World Congress on Targeting Microbiota, 61-61. https://hdl.handle.net/21.15107/rcub_imagine_2186
Brdarić E, Soković Bajić S, Popović D, Kulaš J, Popov Aleksandrov A, Mirkov I, Živković M. CONSUMPTION OF EXOPOLYSACCHARIDES FROM LACTIPLANTIBACILLUS PLANTARUM BGAN8 ALTERS THE GUT MICROBIOTA OF DA RAT. in 10th ISM World Congress on Targeting Microbiota. 2023;:61-61. https://hdl.handle.net/21.15107/rcub_imagine_2186 .
Brdarić, Emilija, Soković Bajić, Svetlana, Popović, Dušanka, Kulaš, Jelena, Popov Aleksandrov, Aleksandra, Mirkov, Ivana, Živković, Milica, "CONSUMPTION OF EXOPOLYSACCHARIDES FROM LACTIPLANTIBACILLUS PLANTARUM BGAN8 ALTERS THE GUT MICROBIOTA OF DA RAT" in 10th ISM World Congress on Targeting Microbiota (2023):61-61, https://hdl.handle.net/21.15107/rcub_imagine_2186 .