EXOPOLYSACCHARIDE-PRODUCING GUT BACTERIA MODULATE HOST AGEING
2023
Аутори
Dinić, MiroslavJakovljević, Stefan
Radojević, Dušan
Brdarić, Emilija
Bajić, Svetlana
Đokić, Jelena
Golić, Nataša
Конференцијски прилог (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Introduction: New theories of ageing recognized gut microbiota as one of twelve hallmarks of ageing (1).
Recent data conducted on Caenorhabditis elegans imply a potential role of Lactobacillus species and other
commensal bacteria
in regulation of ageing highlighting TFEB/HLH-30-dependent autophagy, p38
MAPK/PMK-1 signalling and mitochondrial function as activated longevity-associated mechanisms (2,3).
Here, we explore the potential of bacterial polysaccharides
loosely attached to bacterial cell wall
(exopolysaccharides), considering it is still unknown which bacterial molecules could activate longevity
signalling pathways.
Materials & Methods: Caenorhabditis elegans was used as ageing model. Evaluation of worm’s lifespan
and locomotion rate were performed by feeding worms with six exopolysaccharide-producing lactobacilli.
Worms fed with two selected strains were subjected to RNAseq analysis. Identified upregulated genes were
confirmed by qPCR and expression of th...eir mammalian orthologs checked in human HepG2 cell.
Results: Two strains of lactobacilli showed the most pronounced effect on worms’ lifespan. RNAseq analysis
identified core gene signature associate with exopolysaccharide-induced longevity. The expression of
identified fmo-2, gsto-1, nlp-29, and clec-47 genes were confirmed by qPCR, while upregulation of FMO-5
was confirmed in HepG2 cells.
Conclusion: Overall, our results imply that bacteria-derived exopolysaccharides could stimulate longevity-
promoting flavin-containing monooxygenase 2 to regulate lifespan in Caenorhabditis elegans
Извор:
10th ISM World Congress on Targeting Microbiota, 2023, 65-65Финансирање / пројекти:
- Министарство науке, технолошког развоја и иновација Републике Србије, институционално финансирање - 200042 (Универзитет у Београду, Институт за молекуларну генетику и генетичко инжењерство) (RS-MESTD-inst-2020-200042)
Напомена:
- International Society of Microbiota 10th ISM World Congress on Targeting Microbiota October 17-19, 2023 – Venice, Italy
Институција/група
Institut za molekularnu genetiku i genetičko inženjerstvoTY - CONF AU - Dinić, Miroslav AU - Jakovljević, Stefan AU - Radojević, Dušan AU - Brdarić, Emilija AU - Bajić, Svetlana AU - Đokić, Jelena AU - Golić, Nataša PY - 2023 UR - https://www.microbiota-site.com/ UR - https://imagine.imgge.bg.ac.rs/handle/123456789/2189 AB - Introduction: New theories of ageing recognized gut microbiota as one of twelve hallmarks of ageing (1). Recent data conducted on Caenorhabditis elegans imply a potential role of Lactobacillus species and other commensal bacteria in regulation of ageing highlighting TFEB/HLH-30-dependent autophagy, p38 MAPK/PMK-1 signalling and mitochondrial function as activated longevity-associated mechanisms (2,3). Here, we explore the potential of bacterial polysaccharides loosely attached to bacterial cell wall (exopolysaccharides), considering it is still unknown which bacterial molecules could activate longevity signalling pathways. Materials & Methods: Caenorhabditis elegans was used as ageing model. Evaluation of worm’s lifespan and locomotion rate were performed by feeding worms with six exopolysaccharide-producing lactobacilli. Worms fed with two selected strains were subjected to RNAseq analysis. Identified upregulated genes were confirmed by qPCR and expression of their mammalian orthologs checked in human HepG2 cell. Results: Two strains of lactobacilli showed the most pronounced effect on worms’ lifespan. RNAseq analysis identified core gene signature associate with exopolysaccharide-induced longevity. The expression of identified fmo-2, gsto-1, nlp-29, and clec-47 genes were confirmed by qPCR, while upregulation of FMO-5 was confirmed in HepG2 cells. Conclusion: Overall, our results imply that bacteria-derived exopolysaccharides could stimulate longevity- promoting flavin-containing monooxygenase 2 to regulate lifespan in Caenorhabditis elegans C3 - 10th ISM World Congress on Targeting Microbiota T1 - EXOPOLYSACCHARIDE-PRODUCING GUT BACTERIA MODULATE HOST AGEING EP - 65 SP - 65 UR - https://hdl.handle.net/21.15107/rcub_imagine_2189 ER -
@conference{ author = "Dinić, Miroslav and Jakovljević, Stefan and Radojević, Dušan and Brdarić, Emilija and Bajić, Svetlana and Đokić, Jelena and Golić, Nataša", year = "2023", abstract = "Introduction: New theories of ageing recognized gut microbiota as one of twelve hallmarks of ageing (1). Recent data conducted on Caenorhabditis elegans imply a potential role of Lactobacillus species and other commensal bacteria in regulation of ageing highlighting TFEB/HLH-30-dependent autophagy, p38 MAPK/PMK-1 signalling and mitochondrial function as activated longevity-associated mechanisms (2,3). Here, we explore the potential of bacterial polysaccharides loosely attached to bacterial cell wall (exopolysaccharides), considering it is still unknown which bacterial molecules could activate longevity signalling pathways. Materials & Methods: Caenorhabditis elegans was used as ageing model. Evaluation of worm’s lifespan and locomotion rate were performed by feeding worms with six exopolysaccharide-producing lactobacilli. Worms fed with two selected strains were subjected to RNAseq analysis. Identified upregulated genes were confirmed by qPCR and expression of their mammalian orthologs checked in human HepG2 cell. Results: Two strains of lactobacilli showed the most pronounced effect on worms’ lifespan. RNAseq analysis identified core gene signature associate with exopolysaccharide-induced longevity. The expression of identified fmo-2, gsto-1, nlp-29, and clec-47 genes were confirmed by qPCR, while upregulation of FMO-5 was confirmed in HepG2 cells. Conclusion: Overall, our results imply that bacteria-derived exopolysaccharides could stimulate longevity- promoting flavin-containing monooxygenase 2 to regulate lifespan in Caenorhabditis elegans", journal = "10th ISM World Congress on Targeting Microbiota", title = "EXOPOLYSACCHARIDE-PRODUCING GUT BACTERIA MODULATE HOST AGEING", pages = "65-65", url = "https://hdl.handle.net/21.15107/rcub_imagine_2189" }
Dinić, M., Jakovljević, S., Radojević, D., Brdarić, E., Bajić, S., Đokić, J.,& Golić, N.. (2023). EXOPOLYSACCHARIDE-PRODUCING GUT BACTERIA MODULATE HOST AGEING. in 10th ISM World Congress on Targeting Microbiota, 65-65. https://hdl.handle.net/21.15107/rcub_imagine_2189
Dinić M, Jakovljević S, Radojević D, Brdarić E, Bajić S, Đokić J, Golić N. EXOPOLYSACCHARIDE-PRODUCING GUT BACTERIA MODULATE HOST AGEING. in 10th ISM World Congress on Targeting Microbiota. 2023;:65-65. https://hdl.handle.net/21.15107/rcub_imagine_2189 .
Dinić, Miroslav, Jakovljević, Stefan, Radojević, Dušan, Brdarić, Emilija, Bajić, Svetlana, Đokić, Jelena, Golić, Nataša, "EXOPOLYSACCHARIDE-PRODUCING GUT BACTERIA MODULATE HOST AGEING" in 10th ISM World Congress on Targeting Microbiota (2023):65-65, https://hdl.handle.net/21.15107/rcub_imagine_2189 .