Fresh hyperalkaline groundwaters contain polyurethane-degrading Flavobacterium
Апстракт
The examined groundwaters, orginating from the fractured type of aquifer formed by Pre-
Middle Jurassic ultramafic rocks, i. e. harzburgites, dunites, lherzolites and serpentinites, belong to the
fresh hyperalkaline cold groundwaters of OH, Cl – Ca, Na + K genetic type. Studied occurrence was
characterized by the presence of aerobic cultivable bacteria with ability to grow in a pH range of 7-12.
Bacterial isolates were screened using plastic,cellulosic and hemicellulosic polymeric substrates as
predominantcarbon source to assess their biotechnological potential. All 16 screened isolates (100%)
demonstrated growth and/or activity on at least one tested substrate. One isolate belonging to genus
Flavobacterium, produced prominent zone of clearing on Impranil® DLN-SD, a model substrate for
polyurethanes (PU). Plastic-degrading potential has been previously documented for members of
genus Flavobacterium. However, this is the first report of their PU-degrading activity. Accumulati...on of
PU in the environment is of special concern, due to its toxicity, high production rates and lack of
efficient recycling strategies. In the future, biotechnological potential of alkalophilic bacteria from
groundwater environments should be explored more intensly, especially from the aspect of their
application in the management of plastic waste and bioremediation treatments of plastic-polluted
groundwater resources.
Кључне речи:
words:fresh hyperalkaline groundwaters / 16S rDNA sequencing / polyurethane-degrading FlavobacteriumИзвор:
XIII International Conference - Industrial Engineering and Environmental Protection, 2023, 357-361Издавач:
- Zrenjanin : Technical Faculty "Mihajlo Pupin",
Финансирање / пројекти:
- European Union’s H2020 Research and Innovation Programme under grant agreement No. 870292 (BioICEP)
- Министарство науке, технолошког развоја и иновација Републике Србије, институционално финансирање - 200042 (Универзитет у Београду, Институт за молекуларну генетику и генетичко инжењерство) (RS-MESTD-inst-2020-200042)
Напомена:
- XIII International Conference Industrial Engineering and Environmental Protection (IIZS 2023), Zrenjanin, 5-6th October 2023.
Институција/група
Institut za molekularnu genetiku i genetičko inženjerstvoTY - CONF AU - Šaraba, Vladimir AU - Nikodinović-Runić, Jasmina AU - Ćirić, Milica PY - 2023 UR - https://imagine.imgge.bg.ac.rs/handle/123456789/2203 AB - The examined groundwaters, orginating from the fractured type of aquifer formed by Pre- Middle Jurassic ultramafic rocks, i. e. harzburgites, dunites, lherzolites and serpentinites, belong to the fresh hyperalkaline cold groundwaters of OH, Cl – Ca, Na + K genetic type. Studied occurrence was characterized by the presence of aerobic cultivable bacteria with ability to grow in a pH range of 7-12. Bacterial isolates were screened using plastic,cellulosic and hemicellulosic polymeric substrates as predominantcarbon source to assess their biotechnological potential. All 16 screened isolates (100%) demonstrated growth and/or activity on at least one tested substrate. One isolate belonging to genus Flavobacterium, produced prominent zone of clearing on Impranil® DLN-SD, a model substrate for polyurethanes (PU). Plastic-degrading potential has been previously documented for members of genus Flavobacterium. However, this is the first report of their PU-degrading activity. Accumulation of PU in the environment is of special concern, due to its toxicity, high production rates and lack of efficient recycling strategies. In the future, biotechnological potential of alkalophilic bacteria from groundwater environments should be explored more intensly, especially from the aspect of their application in the management of plastic waste and bioremediation treatments of plastic-polluted groundwater resources. PB - Zrenjanin : Technical Faculty "Mihajlo Pupin", C3 - XIII International Conference - Industrial Engineering and Environmental Protection T1 - Fresh hyperalkaline groundwaters contain polyurethane-degrading Flavobacterium EP - 361 SP - 357 UR - https://hdl.handle.net/21.15107/rcub_imagine_2203 ER -
@conference{ author = "Šaraba, Vladimir and Nikodinović-Runić, Jasmina and Ćirić, Milica", year = "2023", abstract = "The examined groundwaters, orginating from the fractured type of aquifer formed by Pre- Middle Jurassic ultramafic rocks, i. e. harzburgites, dunites, lherzolites and serpentinites, belong to the fresh hyperalkaline cold groundwaters of OH, Cl – Ca, Na + K genetic type. Studied occurrence was characterized by the presence of aerobic cultivable bacteria with ability to grow in a pH range of 7-12. Bacterial isolates were screened using plastic,cellulosic and hemicellulosic polymeric substrates as predominantcarbon source to assess their biotechnological potential. All 16 screened isolates (100%) demonstrated growth and/or activity on at least one tested substrate. One isolate belonging to genus Flavobacterium, produced prominent zone of clearing on Impranil® DLN-SD, a model substrate for polyurethanes (PU). Plastic-degrading potential has been previously documented for members of genus Flavobacterium. However, this is the first report of their PU-degrading activity. Accumulation of PU in the environment is of special concern, due to its toxicity, high production rates and lack of efficient recycling strategies. In the future, biotechnological potential of alkalophilic bacteria from groundwater environments should be explored more intensly, especially from the aspect of their application in the management of plastic waste and bioremediation treatments of plastic-polluted groundwater resources.", publisher = "Zrenjanin : Technical Faculty "Mihajlo Pupin",", journal = "XIII International Conference - Industrial Engineering and Environmental Protection", title = "Fresh hyperalkaline groundwaters contain polyurethane-degrading Flavobacterium", pages = "361-357", url = "https://hdl.handle.net/21.15107/rcub_imagine_2203" }
Šaraba, V., Nikodinović-Runić, J.,& Ćirić, M.. (2023). Fresh hyperalkaline groundwaters contain polyurethane-degrading Flavobacterium. in XIII International Conference - Industrial Engineering and Environmental Protection Zrenjanin : Technical Faculty "Mihajlo Pupin",., 357-361. https://hdl.handle.net/21.15107/rcub_imagine_2203
Šaraba V, Nikodinović-Runić J, Ćirić M. Fresh hyperalkaline groundwaters contain polyurethane-degrading Flavobacterium. in XIII International Conference - Industrial Engineering and Environmental Protection. 2023;:357-361. https://hdl.handle.net/21.15107/rcub_imagine_2203 .
Šaraba, Vladimir, Nikodinović-Runić, Jasmina, Ćirić, Milica, "Fresh hyperalkaline groundwaters contain polyurethane-degrading Flavobacterium" in XIII International Conference - Industrial Engineering and Environmental Protection (2023):357-361, https://hdl.handle.net/21.15107/rcub_imagine_2203 .