Effect of enriched environment on serotonin and RNA editing of serotonin 2C receptor is specific for brain regions and mouse strains
Аутори
Karanović, JelenaBratkovič, Tomaž
Stamenković, Vera
Jasnić, Nebojša
Milošević, Milena
Đorđević, Ana
Anđus, Pavle
Jovanović, Vladimir
Savić- Pavićević, Dušanka
Конференцијски прилог (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Serotonin neurotransmission is sensitive to environmental stimuli. Serotonin receptor
2C (HTR2C) undergoes dynamic A-to-I editing that fine-tunes cell response to
serotonin and is altered in depressed suicide victims and by pharmacological
treatments. We aimed to explore a mediating role of Htr2c mRNA editing in response
to enriched environment and factors involved in this response. Three-week-old
BALB/c and C57BL/6 male mice were housed in enriched and standard conditions for
four weeks. Htr2c mRNA editing pattern and expression, serotonin level and
expression of Adar and Adarb1 mRNAs (coding enzymes catalyzing A-to-I editing)
and Snord115 RNA (regulating Htr2c mRNA alternative splicing and editing) were
measured in prefrontal cortex (PFC) and hippocampus (HC), brain regions implicated
in suicidal behavior. BALB/c mice, a “stress-sensitive” strain due to genetically
determined lower serotonin level, responded to enriched conditions by adapting the
Htr2c editing pattern to ...a slight serotonin decrease in PFC and a significant increase
in HC. C57BL/6 mice, a “stress-resilient” strain, responded to enriched environment
by increasing the serotonin level and changing Adar and Adarb1 mRNAs expression
in HC, and without changes in PFC. Our findings suggest that the enriched
environment effect on the serotonin level and a mediating role of Htr2c mRNA
editing in PFC depend on the genetic background and its interactions with the
environment. On the other hand, changes in HC are primarily driven by enriched
environment.
Our results imply usefulness of enriched environment paradigm for understanding
interactions of genetic and environmental factors underlying suicidal behavior, which
might improve psychological treatments.
Извор:
8th Congress of the Serbian Neuroscience Society, 2023, 46-46Издавач:
- Belgrade : Serbian Neuroscience Society
Финансирање / пројекти:
- This study was supported by the Ministry of Education, Science and Technological Development, Republic of Serbia and Slovenian Research Agency (Agreement no. 451-03-39/2016-09/15/01)
Напомена:
- BOOK OF ABSTRACTS: 8th CONGRESS OF SERBIAN NEUROSCIENCE SOCIETY with international participation 31 May – 2 June 2023. Belgrade, Serbia
Институција/група
Institut za molekularnu genetiku i genetičko inženjerstvoTY - CONF AU - Karanović, Jelena AU - Bratkovič, Tomaž AU - Stamenković, Vera AU - Jasnić, Nebojša AU - Milošević, Milena AU - Đorđević, Ana AU - Anđus, Pavle AU - Jovanović, Vladimir AU - Savić- Pavićević, Dušanka PY - 2023 UR - https://imagine.imgge.bg.ac.rs/handle/123456789/2215 AB - Serotonin neurotransmission is sensitive to environmental stimuli. Serotonin receptor 2C (HTR2C) undergoes dynamic A-to-I editing that fine-tunes cell response to serotonin and is altered in depressed suicide victims and by pharmacological treatments. We aimed to explore a mediating role of Htr2c mRNA editing in response to enriched environment and factors involved in this response. Three-week-old BALB/c and C57BL/6 male mice were housed in enriched and standard conditions for four weeks. Htr2c mRNA editing pattern and expression, serotonin level and expression of Adar and Adarb1 mRNAs (coding enzymes catalyzing A-to-I editing) and Snord115 RNA (regulating Htr2c mRNA alternative splicing and editing) were measured in prefrontal cortex (PFC) and hippocampus (HC), brain regions implicated in suicidal behavior. BALB/c mice, a “stress-sensitive” strain due to genetically determined lower serotonin level, responded to enriched conditions by adapting the Htr2c editing pattern to a slight serotonin decrease in PFC and a significant increase in HC. C57BL/6 mice, a “stress-resilient” strain, responded to enriched environment by increasing the serotonin level and changing Adar and Adarb1 mRNAs expression in HC, and without changes in PFC. Our findings suggest that the enriched environment effect on the serotonin level and a mediating role of Htr2c mRNA editing in PFC depend on the genetic background and its interactions with the environment. On the other hand, changes in HC are primarily driven by enriched environment. Our results imply usefulness of enriched environment paradigm for understanding interactions of genetic and environmental factors underlying suicidal behavior, which might improve psychological treatments. PB - Belgrade : Serbian Neuroscience Society C3 - 8th Congress of the Serbian Neuroscience Society T1 - Effect of enriched environment on serotonin and RNA editing of serotonin 2C receptor is specific for brain regions and mouse strains EP - 46 SP - 46 UR - https://hdl.handle.net/21.15107/rcub_imagine_2215 ER -
@conference{ author = "Karanović, Jelena and Bratkovič, Tomaž and Stamenković, Vera and Jasnić, Nebojša and Milošević, Milena and Đorđević, Ana and Anđus, Pavle and Jovanović, Vladimir and Savić- Pavićević, Dušanka", year = "2023", abstract = "Serotonin neurotransmission is sensitive to environmental stimuli. Serotonin receptor 2C (HTR2C) undergoes dynamic A-to-I editing that fine-tunes cell response to serotonin and is altered in depressed suicide victims and by pharmacological treatments. We aimed to explore a mediating role of Htr2c mRNA editing in response to enriched environment and factors involved in this response. Three-week-old BALB/c and C57BL/6 male mice were housed in enriched and standard conditions for four weeks. Htr2c mRNA editing pattern and expression, serotonin level and expression of Adar and Adarb1 mRNAs (coding enzymes catalyzing A-to-I editing) and Snord115 RNA (regulating Htr2c mRNA alternative splicing and editing) were measured in prefrontal cortex (PFC) and hippocampus (HC), brain regions implicated in suicidal behavior. BALB/c mice, a “stress-sensitive” strain due to genetically determined lower serotonin level, responded to enriched conditions by adapting the Htr2c editing pattern to a slight serotonin decrease in PFC and a significant increase in HC. C57BL/6 mice, a “stress-resilient” strain, responded to enriched environment by increasing the serotonin level and changing Adar and Adarb1 mRNAs expression in HC, and without changes in PFC. Our findings suggest that the enriched environment effect on the serotonin level and a mediating role of Htr2c mRNA editing in PFC depend on the genetic background and its interactions with the environment. On the other hand, changes in HC are primarily driven by enriched environment. Our results imply usefulness of enriched environment paradigm for understanding interactions of genetic and environmental factors underlying suicidal behavior, which might improve psychological treatments.", publisher = "Belgrade : Serbian Neuroscience Society", journal = "8th Congress of the Serbian Neuroscience Society", title = "Effect of enriched environment on serotonin and RNA editing of serotonin 2C receptor is specific for brain regions and mouse strains", pages = "46-46", url = "https://hdl.handle.net/21.15107/rcub_imagine_2215" }
Karanović, J., Bratkovič, T., Stamenković, V., Jasnić, N., Milošević, M., Đorđević, A., Anđus, P., Jovanović, V.,& Savić- Pavićević, D.. (2023). Effect of enriched environment on serotonin and RNA editing of serotonin 2C receptor is specific for brain regions and mouse strains. in 8th Congress of the Serbian Neuroscience Society Belgrade : Serbian Neuroscience Society., 46-46. https://hdl.handle.net/21.15107/rcub_imagine_2215
Karanović J, Bratkovič T, Stamenković V, Jasnić N, Milošević M, Đorđević A, Anđus P, Jovanović V, Savić- Pavićević D. Effect of enriched environment on serotonin and RNA editing of serotonin 2C receptor is specific for brain regions and mouse strains. in 8th Congress of the Serbian Neuroscience Society. 2023;:46-46. https://hdl.handle.net/21.15107/rcub_imagine_2215 .
Karanović, Jelena, Bratkovič, Tomaž, Stamenković, Vera, Jasnić, Nebojša, Milošević, Milena, Đorđević, Ana, Anđus, Pavle, Jovanović, Vladimir, Savić- Pavićević, Dušanka, "Effect of enriched environment on serotonin and RNA editing of serotonin 2C receptor is specific for brain regions and mouse strains" in 8th Congress of the Serbian Neuroscience Society (2023):46-46, https://hdl.handle.net/21.15107/rcub_imagine_2215 .