Exploring the enzymatic ability of strains isolated from plastic-polluted environments for enhancing synthetic and natural biopolymers’ biodegradation
Апстракт
Background: Renowned oil-based polymers represent
an environmental nuisance, due to their well-known
permanency in nature, known to disrupt ecosystems.
In response, bio-based polymers have emerged as an
alternative, providing a sustainable blueprint for
plastic circularity1. However, despite being classified
as biodegradable, these polymers can still show
resistance to biodegradation when not presented
with optimal environmental conditions such as
specific temperature and pH, amongst other key
factors in their breakdown. Due to this, microbial
degradation results in an interesting path to promote
biodegradation of such polymers.2
Objective: This study aims to evaluate the ability of
selected strains isolated from polluted environments
to improve the rate of biopolymers’ biodegradation
such as Polyhydroxy butyrate (PHB), Polylactic acid
(PLA), and Bacterial cellulose (BC) when exposed to
strains with specific enzymatic tools to aid in its
degradation.
Methods: Select...ed strains were previously isolated
from plastic-polluted soil samples. Strains were
transferred to Mineral Salt media (MSM) biopolymersupplemented
plates (7d, 30℃) (MSM (15 g/l Agar, 9
g/l Na2HPO4 x 12H2O, 1.5 g/l KH2PO4, 1 g/l NH4Cl,
0.2 g/l MgSO4 x 7H2O, 0.2 g/l CaCl2 x 2H2O,
Fe(III)NH4-citrate 0.0012 g/l), (1-3%) biopolymers
(PHB, BC, and PLA) growth and clear-zone method
were used to determine strains’ possible enzymatic
activity. Selected strains were transferred to flasks
containing PHB, PLA, and BC films, respectively, in
MSM and incubated for 20 days at 30℃, 120 rpm.
Samples were washed and dried and their level of
degradation was assessed by FTIR, weight loss, and
scanning electron microscopy (SEM). The ability of the
best performer strain to degrade PHB was
additionally assessed using Respirometer (Echo
Instruments) to determine the biodegradability of
said films under a controlled temperature (25°C) with
a flow rate of 500ml/min.
Results: Significant weight loss was observed in
samples exposed to strains, meaning biodegradability
was achieved in an important percentage, proving
their capability to degrade the proposed
biopolymers, compared to samples lacking microbial
presence.
Кључне речи:
natural biopolymers / polymersИзвор:
10th Conference of Mikrobiokosmos,, 2023Напомена:
- 10th International Conference of MIKROBIOKOSMOS, Larissa from 30 Novewmber to 2 December 2023.
URI
https://afea.eventsair.com/10th-conference-of-mikrobiokosmos/abstract-bookhttps://imagine.imgge.bg.ac.rs/handle/123456789/2234
Институција/група
Institut za molekularnu genetiku i genetičko inženjerstvoTY - CONF AU - Garza Herrera, Diana Alicia AU - Mojicević, Marija AU - Nikodinović-Runić, Jasmina PY - 2023 UR - https://afea.eventsair.com/10th-conference-of-mikrobiokosmos/abstract-book UR - https://imagine.imgge.bg.ac.rs/handle/123456789/2234 AB - Background: Renowned oil-based polymers represent an environmental nuisance, due to their well-known permanency in nature, known to disrupt ecosystems. In response, bio-based polymers have emerged as an alternative, providing a sustainable blueprint for plastic circularity1. However, despite being classified as biodegradable, these polymers can still show resistance to biodegradation when not presented with optimal environmental conditions such as specific temperature and pH, amongst other key factors in their breakdown. Due to this, microbial degradation results in an interesting path to promote biodegradation of such polymers.2 Objective: This study aims to evaluate the ability of selected strains isolated from polluted environments to improve the rate of biopolymers’ biodegradation such as Polyhydroxy butyrate (PHB), Polylactic acid (PLA), and Bacterial cellulose (BC) when exposed to strains with specific enzymatic tools to aid in its degradation. Methods: Selected strains were previously isolated from plastic-polluted soil samples. Strains were transferred to Mineral Salt media (MSM) biopolymersupplemented plates (7d, 30℃) (MSM (15 g/l Agar, 9 g/l Na2HPO4 x 12H2O, 1.5 g/l KH2PO4, 1 g/l NH4Cl, 0.2 g/l MgSO4 x 7H2O, 0.2 g/l CaCl2 x 2H2O, Fe(III)NH4-citrate 0.0012 g/l), (1-3%) biopolymers (PHB, BC, and PLA) growth and clear-zone method were used to determine strains’ possible enzymatic activity. Selected strains were transferred to flasks containing PHB, PLA, and BC films, respectively, in MSM and incubated for 20 days at 30℃, 120 rpm. Samples were washed and dried and their level of degradation was assessed by FTIR, weight loss, and scanning electron microscopy (SEM). The ability of the best performer strain to degrade PHB was additionally assessed using Respirometer (Echo Instruments) to determine the biodegradability of said films under a controlled temperature (25°C) with a flow rate of 500ml/min. Results: Significant weight loss was observed in samples exposed to strains, meaning biodegradability was achieved in an important percentage, proving their capability to degrade the proposed biopolymers, compared to samples lacking microbial presence. C3 - 10th Conference of Mikrobiokosmos, T1 - Exploring the enzymatic ability of strains isolated from plastic-polluted environments for enhancing synthetic and natural biopolymers’ biodegradation UR - https://hdl.handle.net/21.15107/rcub_imagine_2234 ER -
@conference{ author = "Garza Herrera, Diana Alicia and Mojicević, Marija and Nikodinović-Runić, Jasmina", year = "2023", abstract = "Background: Renowned oil-based polymers represent an environmental nuisance, due to their well-known permanency in nature, known to disrupt ecosystems. In response, bio-based polymers have emerged as an alternative, providing a sustainable blueprint for plastic circularity1. However, despite being classified as biodegradable, these polymers can still show resistance to biodegradation when not presented with optimal environmental conditions such as specific temperature and pH, amongst other key factors in their breakdown. Due to this, microbial degradation results in an interesting path to promote biodegradation of such polymers.2 Objective: This study aims to evaluate the ability of selected strains isolated from polluted environments to improve the rate of biopolymers’ biodegradation such as Polyhydroxy butyrate (PHB), Polylactic acid (PLA), and Bacterial cellulose (BC) when exposed to strains with specific enzymatic tools to aid in its degradation. Methods: Selected strains were previously isolated from plastic-polluted soil samples. Strains were transferred to Mineral Salt media (MSM) biopolymersupplemented plates (7d, 30℃) (MSM (15 g/l Agar, 9 g/l Na2HPO4 x 12H2O, 1.5 g/l KH2PO4, 1 g/l NH4Cl, 0.2 g/l MgSO4 x 7H2O, 0.2 g/l CaCl2 x 2H2O, Fe(III)NH4-citrate 0.0012 g/l), (1-3%) biopolymers (PHB, BC, and PLA) growth and clear-zone method were used to determine strains’ possible enzymatic activity. Selected strains were transferred to flasks containing PHB, PLA, and BC films, respectively, in MSM and incubated for 20 days at 30℃, 120 rpm. Samples were washed and dried and their level of degradation was assessed by FTIR, weight loss, and scanning electron microscopy (SEM). The ability of the best performer strain to degrade PHB was additionally assessed using Respirometer (Echo Instruments) to determine the biodegradability of said films under a controlled temperature (25°C) with a flow rate of 500ml/min. Results: Significant weight loss was observed in samples exposed to strains, meaning biodegradability was achieved in an important percentage, proving their capability to degrade the proposed biopolymers, compared to samples lacking microbial presence.", journal = "10th Conference of Mikrobiokosmos,", title = "Exploring the enzymatic ability of strains isolated from plastic-polluted environments for enhancing synthetic and natural biopolymers’ biodegradation", url = "https://hdl.handle.net/21.15107/rcub_imagine_2234" }
Garza Herrera, D. A., Mojicević, M.,& Nikodinović-Runić, J.. (2023). Exploring the enzymatic ability of strains isolated from plastic-polluted environments for enhancing synthetic and natural biopolymers’ biodegradation. in 10th Conference of Mikrobiokosmos,. https://hdl.handle.net/21.15107/rcub_imagine_2234
Garza Herrera DA, Mojicević M, Nikodinović-Runić J. Exploring the enzymatic ability of strains isolated from plastic-polluted environments for enhancing synthetic and natural biopolymers’ biodegradation. in 10th Conference of Mikrobiokosmos,. 2023;. https://hdl.handle.net/21.15107/rcub_imagine_2234 .
Garza Herrera, Diana Alicia, Mojicević, Marija, Nikodinović-Runić, Jasmina, "Exploring the enzymatic ability of strains isolated from plastic-polluted environments for enhancing synthetic and natural biopolymers’ biodegradation" in 10th Conference of Mikrobiokosmos, (2023), https://hdl.handle.net/21.15107/rcub_imagine_2234 .