Biotechnological production of bacterial pigment prodigiosin and bioactive properties of its metal complexes with Cu(II) and Zn(II)
Аутори
Lazić, JelenaMilovanović, Jelena
Anejević, Tina
Ašanin, Darko
Ilić-Tomić, Tatjana
Glišić, Biljana
Nikodinović-Runić, Jasmina
Конференцијски прилог (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Prodigiosin (PG, Fig. 1a) is a biologically active
pyrrolylpyrromethene alkaloid whose structure was
first confirmed in 1962 [1]. PG is commonly
produced by Gram-negative bacteria, such as
Serratia spp. and has an eco-physiological role [2].
Its biological activities were extensively researched,
and numerous pharmacological properties were
established, including anticancer and
immunosuppressive However, high cost of extraction and purification
still represent the bottleneck in the microbial
production of PG. Meat and fish processing wastes have high potential as raw materials for conversion
into useful products of higher value. In this study,
meat offcuts were assessed as the sole nutrient for
the fermentative production of PG from S.
marcescens. Using this substrate lowered the
cultivation medium cost and shortened the
fermentation time to 12 h, while allowing a
satisfying PG yield of 83.1 mg/L. The isolated PG
was used in one-step reactions with CuCl₂ or ZnCl₂
in t...erc-BuOH at 25 °C. The obtained [Cu(PG)Cl] (Fig.
1b) and [Zn(PG)₂] (Fig. 1c) complexes were
characterized by UV-Vis and IR spectroscopy and
their bioactivity potential was assessed.
Antimicrobial activity was assessed in a disc assay
against 4 human pathogens: Escherichia coli NCTC
9001, Pseudomonas aeruginosa ATCC 10332,
Staphylococcus aureus NCTC 6571, Candida
albicans ATCC 10231, but no effect was observed
for the tested concentrations of 200 μg per disc and
lower. However, the anticancer potential of the new
derivatives is promising and the bovine serum
albumin (BSA) binding study revealed that
complexes bind to BSA tightly and reversibly [4].
Кључне речи:
bacterial pigmentsИзвор:
10th Conference of Mikrobiokosmos, 2023Финансирање / пројекти:
- BioECOLogics - Value-added biologics through eco-sustainable routes (RS-ScienceFundRS-Ideje-7730810)
Напомена:
- 10th International Conference of MIKROBIOKOSMOS, Larissa from 30 Novewmber to 2 December 2023.
URI
https://afea.eventsair.com/10th-conference-of-mikrobiokosmos/abstract-bookhttps://imagine.imgge.bg.ac.rs/handle/123456789/2235
Институција/група
Institut za molekularnu genetiku i genetičko inženjerstvoTY - CONF AU - Lazić, Jelena AU - Milovanović, Jelena AU - Anejević, Tina AU - Ašanin, Darko AU - Ilić-Tomić, Tatjana AU - Glišić, Biljana AU - Nikodinović-Runić, Jasmina PY - 2023 UR - https://afea.eventsair.com/10th-conference-of-mikrobiokosmos/abstract-book UR - https://imagine.imgge.bg.ac.rs/handle/123456789/2235 AB - Prodigiosin (PG, Fig. 1a) is a biologically active pyrrolylpyrromethene alkaloid whose structure was first confirmed in 1962 [1]. PG is commonly produced by Gram-negative bacteria, such as Serratia spp. and has an eco-physiological role [2]. Its biological activities were extensively researched, and numerous pharmacological properties were established, including anticancer and immunosuppressive However, high cost of extraction and purification still represent the bottleneck in the microbial production of PG. Meat and fish processing wastes have high potential as raw materials for conversion into useful products of higher value. In this study, meat offcuts were assessed as the sole nutrient for the fermentative production of PG from S. marcescens. Using this substrate lowered the cultivation medium cost and shortened the fermentation time to 12 h, while allowing a satisfying PG yield of 83.1 mg/L. The isolated PG was used in one-step reactions with CuCl₂ or ZnCl₂ in terc-BuOH at 25 °C. The obtained [Cu(PG)Cl] (Fig. 1b) and [Zn(PG)₂] (Fig. 1c) complexes were characterized by UV-Vis and IR spectroscopy and their bioactivity potential was assessed. Antimicrobial activity was assessed in a disc assay against 4 human pathogens: Escherichia coli NCTC 9001, Pseudomonas aeruginosa ATCC 10332, Staphylococcus aureus NCTC 6571, Candida albicans ATCC 10231, but no effect was observed for the tested concentrations of 200 μg per disc and lower. However, the anticancer potential of the new derivatives is promising and the bovine serum albumin (BSA) binding study revealed that complexes bind to BSA tightly and reversibly [4]. C3 - 10th Conference of Mikrobiokosmos T1 - Biotechnological production of bacterial pigment prodigiosin and bioactive properties of its metal complexes with Cu(II) and Zn(II) UR - https://hdl.handle.net/21.15107/rcub_imagine_2235 ER -
@conference{ author = "Lazić, Jelena and Milovanović, Jelena and Anejević, Tina and Ašanin, Darko and Ilić-Tomić, Tatjana and Glišić, Biljana and Nikodinović-Runić, Jasmina", year = "2023", abstract = "Prodigiosin (PG, Fig. 1a) is a biologically active pyrrolylpyrromethene alkaloid whose structure was first confirmed in 1962 [1]. PG is commonly produced by Gram-negative bacteria, such as Serratia spp. and has an eco-physiological role [2]. Its biological activities were extensively researched, and numerous pharmacological properties were established, including anticancer and immunosuppressive However, high cost of extraction and purification still represent the bottleneck in the microbial production of PG. Meat and fish processing wastes have high potential as raw materials for conversion into useful products of higher value. In this study, meat offcuts were assessed as the sole nutrient for the fermentative production of PG from S. marcescens. Using this substrate lowered the cultivation medium cost and shortened the fermentation time to 12 h, while allowing a satisfying PG yield of 83.1 mg/L. The isolated PG was used in one-step reactions with CuCl₂ or ZnCl₂ in terc-BuOH at 25 °C. The obtained [Cu(PG)Cl] (Fig. 1b) and [Zn(PG)₂] (Fig. 1c) complexes were characterized by UV-Vis and IR spectroscopy and their bioactivity potential was assessed. Antimicrobial activity was assessed in a disc assay against 4 human pathogens: Escherichia coli NCTC 9001, Pseudomonas aeruginosa ATCC 10332, Staphylococcus aureus NCTC 6571, Candida albicans ATCC 10231, but no effect was observed for the tested concentrations of 200 μg per disc and lower. However, the anticancer potential of the new derivatives is promising and the bovine serum albumin (BSA) binding study revealed that complexes bind to BSA tightly and reversibly [4].", journal = "10th Conference of Mikrobiokosmos", title = "Biotechnological production of bacterial pigment prodigiosin and bioactive properties of its metal complexes with Cu(II) and Zn(II)", url = "https://hdl.handle.net/21.15107/rcub_imagine_2235" }
Lazić, J., Milovanović, J., Anejević, T., Ašanin, D., Ilić-Tomić, T., Glišić, B.,& Nikodinović-Runić, J.. (2023). Biotechnological production of bacterial pigment prodigiosin and bioactive properties of its metal complexes with Cu(II) and Zn(II). in 10th Conference of Mikrobiokosmos. https://hdl.handle.net/21.15107/rcub_imagine_2235
Lazić J, Milovanović J, Anejević T, Ašanin D, Ilić-Tomić T, Glišić B, Nikodinović-Runić J. Biotechnological production of bacterial pigment prodigiosin and bioactive properties of its metal complexes with Cu(II) and Zn(II). in 10th Conference of Mikrobiokosmos. 2023;. https://hdl.handle.net/21.15107/rcub_imagine_2235 .
Lazić, Jelena, Milovanović, Jelena, Anejević, Tina, Ašanin, Darko, Ilić-Tomić, Tatjana, Glišić, Biljana, Nikodinović-Runić, Jasmina, "Biotechnological production of bacterial pigment prodigiosin and bioactive properties of its metal complexes with Cu(II) and Zn(II)" in 10th Conference of Mikrobiokosmos (2023), https://hdl.handle.net/21.15107/rcub_imagine_2235 .