Hybridosomes from spruce needle homogenate
Аутори
Spasovski, VesnaRomolo, Anna
Kisovec, Matic
Zagorc, Urška
Arrigler, Vesna
Arko, Matevž
Bedina Zavec, Apolonija
Iglič, Aleš
Kogej, Ksenija
Kralj-Iglič, Veronika
Конференцијски прилог (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Introduction: Being of compatible structure with biomembranes, lipid–based nanoparticles are considered
as convenient platforms for drug delivery systems. In the proposed work we considered formation of lipid
nanovesicles associated with bioactive phytochemicals from spruce needle homogenate (here called
hybridosomes). We formed hybridosomes by mixing appropriate amounts of lecithin, supernatant of isolation
of extracellular particles from spruce needle homogenate and glycerol.
Methods: We visualized hybridosomes by light microscopy and cryogenic transmission electron microscopy
and assessed them by flow cytometry, dynamic light scattering, ultraviolet–visual spectroscopy and
interferometric microscopy.
Results: We found that the particles consisted of a bilayer membrane and a fluid-like interior. Flow cytometry
and interferometric light microscopy measurements showed that the majority of the particles were
nano-sized. Dynamic light scattering and interferometric light micros...copy measurements agreed well with the
determined average hydrodynamic radius of the particles Rh (between 140 and 180 nm) while their number
densities were in the range between 10^13 and 10^14/mL indicating that hybridosomes present about 2/3 of the
mixture, excluding solvent and other small molecules.
Discussion: Simple and low-cost preparation method, non-demanding saving process and efficient
formation procedure suggest that large scale production of hybridosomes from lipids and spruce needle
homogenate is feasible.
Извор:
Small New World 2.0, 2023, 27-27Издавач:
- Serbian Society for Extracellular Vesicles (SrbEVs)
- Austrian Society for Extracellular Vesicles (ASEV)
- Hungarian Society for Extracellular Vesicles (HSEV)
- Slovenian Network for Extracellular Vesicles (SiN-EV)
Напомена:
- Small New World 2.0 4-5 September 2023., Graz, Austria
Институција/група
Institut za molekularnu genetiku i genetičko inženjerstvoTY - CONF AU - Spasovski, Vesna AU - Romolo, Anna AU - Kisovec, Matic AU - Zagorc, Urška AU - Arrigler, Vesna AU - Arko, Matevž AU - Bedina Zavec, Apolonija AU - Iglič, Aleš AU - Kogej, Ksenija AU - Kralj-Iglič, Veronika PY - 2023 UR - https://imagine.imgge.bg.ac.rs/handle/123456789/2264 AB - Introduction: Being of compatible structure with biomembranes, lipid–based nanoparticles are considered as convenient platforms for drug delivery systems. In the proposed work we considered formation of lipid nanovesicles associated with bioactive phytochemicals from spruce needle homogenate (here called hybridosomes). We formed hybridosomes by mixing appropriate amounts of lecithin, supernatant of isolation of extracellular particles from spruce needle homogenate and glycerol. Methods: We visualized hybridosomes by light microscopy and cryogenic transmission electron microscopy and assessed them by flow cytometry, dynamic light scattering, ultraviolet–visual spectroscopy and interferometric microscopy. Results: We found that the particles consisted of a bilayer membrane and a fluid-like interior. Flow cytometry and interferometric light microscopy measurements showed that the majority of the particles were nano-sized. Dynamic light scattering and interferometric light microscopy measurements agreed well with the determined average hydrodynamic radius of the particles Rh (between 140 and 180 nm) while their number densities were in the range between 10^13 and 10^14/mL indicating that hybridosomes present about 2/3 of the mixture, excluding solvent and other small molecules. Discussion: Simple and low-cost preparation method, non-demanding saving process and efficient formation procedure suggest that large scale production of hybridosomes from lipids and spruce needle homogenate is feasible. PB - Serbian Society for Extracellular Vesicles (SrbEVs) PB - Austrian Society for Extracellular Vesicles (ASEV) PB - Hungarian Society for Extracellular Vesicles (HSEV) PB - Slovenian Network for Extracellular Vesicles (SiN-EV) C3 - Small New World 2.0 T1 - Hybridosomes from spruce needle homogenate EP - 27 SP - 27 UR - https://hdl.handle.net/21.15107/rcub_imagine_2264 ER -
@conference{ author = "Spasovski, Vesna and Romolo, Anna and Kisovec, Matic and Zagorc, Urška and Arrigler, Vesna and Arko, Matevž and Bedina Zavec, Apolonija and Iglič, Aleš and Kogej, Ksenija and Kralj-Iglič, Veronika", year = "2023", abstract = "Introduction: Being of compatible structure with biomembranes, lipid–based nanoparticles are considered as convenient platforms for drug delivery systems. In the proposed work we considered formation of lipid nanovesicles associated with bioactive phytochemicals from spruce needle homogenate (here called hybridosomes). We formed hybridosomes by mixing appropriate amounts of lecithin, supernatant of isolation of extracellular particles from spruce needle homogenate and glycerol. Methods: We visualized hybridosomes by light microscopy and cryogenic transmission electron microscopy and assessed them by flow cytometry, dynamic light scattering, ultraviolet–visual spectroscopy and interferometric microscopy. Results: We found that the particles consisted of a bilayer membrane and a fluid-like interior. Flow cytometry and interferometric light microscopy measurements showed that the majority of the particles were nano-sized. Dynamic light scattering and interferometric light microscopy measurements agreed well with the determined average hydrodynamic radius of the particles Rh (between 140 and 180 nm) while their number densities were in the range between 10^13 and 10^14/mL indicating that hybridosomes present about 2/3 of the mixture, excluding solvent and other small molecules. Discussion: Simple and low-cost preparation method, non-demanding saving process and efficient formation procedure suggest that large scale production of hybridosomes from lipids and spruce needle homogenate is feasible.", publisher = "Serbian Society for Extracellular Vesicles (SrbEVs), Austrian Society for Extracellular Vesicles (ASEV), Hungarian Society for Extracellular Vesicles (HSEV), Slovenian Network for Extracellular Vesicles (SiN-EV)", journal = "Small New World 2.0", title = "Hybridosomes from spruce needle homogenate", pages = "27-27", url = "https://hdl.handle.net/21.15107/rcub_imagine_2264" }
Spasovski, V., Romolo, A., Kisovec, M., Zagorc, U., Arrigler, V., Arko, M., Bedina Zavec, A., Iglič, A., Kogej, K.,& Kralj-Iglič, V.. (2023). Hybridosomes from spruce needle homogenate. in Small New World 2.0 Serbian Society for Extracellular Vesicles (SrbEVs)., 27-27. https://hdl.handle.net/21.15107/rcub_imagine_2264
Spasovski V, Romolo A, Kisovec M, Zagorc U, Arrigler V, Arko M, Bedina Zavec A, Iglič A, Kogej K, Kralj-Iglič V. Hybridosomes from spruce needle homogenate. in Small New World 2.0. 2023;:27-27. https://hdl.handle.net/21.15107/rcub_imagine_2264 .
Spasovski, Vesna, Romolo, Anna, Kisovec, Matic, Zagorc, Urška, Arrigler, Vesna, Arko, Matevž, Bedina Zavec, Apolonija, Iglič, Aleš, Kogej, Ksenija, Kralj-Iglič, Veronika, "Hybridosomes from spruce needle homogenate" in Small New World 2.0 (2023):27-27, https://hdl.handle.net/21.15107/rcub_imagine_2264 .