Zebrafish ankrd1a as a common player in heart regeneration and skeletal muscle repair - a new prospect for unlocking regenerative potential of human heart (ZEBARR)
Апстракт
ZEBARR project aims to contribute to better understanding of cellular and molecular
mechanisms of healing injured cardiac and skeletal muscles by investigating a common player
that participates in both processes. As a model organism competent in both regeneration of the
heart and repair of skeletal muscle after injury we will use adult zebrafish. Injured zebrafish
heart gets fully regenerated in 60 days by proliferation of unaffected cardiomyocytes, while
skeletal muscle replace injured tissue by activation of dedicated pool of stem cells called
satellite-like cells, that pass thought transformation to form myofibers. Do these processes
have common players and if they do in which particular molecular event(s) they participate?
Are there any points of intersection? We hypothesized that these players and associated
pathways do exist and might help to unlock endogenous regenerative mechanism of human
heart. In our preliminary experiments, ankrd1a gene, homologue of mammalian ple...iotropic
transcriptional regulator ANKRD1, stood out as a common player in these processes, since it
was found activated in both muscle tissues after the injury (cryoinjury of ventricle wall and stab
wound of skeletal muscle). Deciphering precise role of ankrd1a in different pathways involved
in regeneration of zebrafish heart and repair of skeletal muscle and its contribution to the
regenerative potential of different zebrafish muscles will help us to understand these very
important processes in depth. Moreover, ankrd1a might become strong candidate for
consideration when designing novel strategies for healing human heart after myocardial
infraction.
Experimental work will be organized in five work packages:
WP1. Determination of spatio-temporal profile of ankrd1a expression after injury of adult
zebrafish heart and skeletal muscle.
WP2. Investigation of ankrd1a function in regeneration of adult zebrafish heart.
WP3. Investigation of ankrd1a function in repair of adult zebrafish skeletal muscle.
WP4. Identification of ankrd1a downstream targets and associated pathways involved in
regeneration of heart and repair of skeletal muscle of adult zebrafish
WP5. Testing the mediator role of ankrd1a in the beneficial effect of exercise on regeneration
of heart and repair of skeletal muscle.
Извор:
Science Fund of the Republic of Serbia, Program IDEAS, 2021Финансирање / пројекти:
- ZEBARR - Zebrafish ankrd1a as a common player in heart regeneration and skeletal muscle repair: A new prospect for unlocking regenerative potential of human heart (RS-ScienceFundRS-Ideje-7739807)
Напомена:
- Principal Investigator: Dr Snezana Kojic, IMGGE
- Duration period: 2021-2024
Колекције
Институција/група
Institut za molekularnu genetiku i genetičko inženjerstvoTY - GEN PY - 2021 UR - https://imagine.imgge.bg.ac.rs/handle/123456789/2272 AB - ZEBARR project aims to contribute to better understanding of cellular and molecular mechanisms of healing injured cardiac and skeletal muscles by investigating a common player that participates in both processes. As a model organism competent in both regeneration of the heart and repair of skeletal muscle after injury we will use adult zebrafish. Injured zebrafish heart gets fully regenerated in 60 days by proliferation of unaffected cardiomyocytes, while skeletal muscle replace injured tissue by activation of dedicated pool of stem cells called satellite-like cells, that pass thought transformation to form myofibers. Do these processes have common players and if they do in which particular molecular event(s) they participate? Are there any points of intersection? We hypothesized that these players and associated pathways do exist and might help to unlock endogenous regenerative mechanism of human heart. In our preliminary experiments, ankrd1a gene, homologue of mammalian pleiotropic transcriptional regulator ANKRD1, stood out as a common player in these processes, since it was found activated in both muscle tissues after the injury (cryoinjury of ventricle wall and stab wound of skeletal muscle). Deciphering precise role of ankrd1a in different pathways involved in regeneration of zebrafish heart and repair of skeletal muscle and its contribution to the regenerative potential of different zebrafish muscles will help us to understand these very important processes in depth. Moreover, ankrd1a might become strong candidate for consideration when designing novel strategies for healing human heart after myocardial infraction. Experimental work will be organized in five work packages: WP1. Determination of spatio-temporal profile of ankrd1a expression after injury of adult zebrafish heart and skeletal muscle. WP2. Investigation of ankrd1a function in regeneration of adult zebrafish heart. WP3. Investigation of ankrd1a function in repair of adult zebrafish skeletal muscle. WP4. Identification of ankrd1a downstream targets and associated pathways involved in regeneration of heart and repair of skeletal muscle of adult zebrafish WP5. Testing the mediator role of ankrd1a in the beneficial effect of exercise on regeneration of heart and repair of skeletal muscle. T2 - Science Fund of the Republic of Serbia, Program IDEAS T1 - Zebrafish ankrd1a as a common player in heart regeneration and skeletal muscle repair - a new prospect for unlocking regenerative potential of human heart (ZEBARR) UR - https://hdl.handle.net/21.15107/rcub_imagine_2272 ER -
@misc{ year = "2021", abstract = "ZEBARR project aims to contribute to better understanding of cellular and molecular mechanisms of healing injured cardiac and skeletal muscles by investigating a common player that participates in both processes. As a model organism competent in both regeneration of the heart and repair of skeletal muscle after injury we will use adult zebrafish. Injured zebrafish heart gets fully regenerated in 60 days by proliferation of unaffected cardiomyocytes, while skeletal muscle replace injured tissue by activation of dedicated pool of stem cells called satellite-like cells, that pass thought transformation to form myofibers. Do these processes have common players and if they do in which particular molecular event(s) they participate? Are there any points of intersection? We hypothesized that these players and associated pathways do exist and might help to unlock endogenous regenerative mechanism of human heart. In our preliminary experiments, ankrd1a gene, homologue of mammalian pleiotropic transcriptional regulator ANKRD1, stood out as a common player in these processes, since it was found activated in both muscle tissues after the injury (cryoinjury of ventricle wall and stab wound of skeletal muscle). Deciphering precise role of ankrd1a in different pathways involved in regeneration of zebrafish heart and repair of skeletal muscle and its contribution to the regenerative potential of different zebrafish muscles will help us to understand these very important processes in depth. Moreover, ankrd1a might become strong candidate for consideration when designing novel strategies for healing human heart after myocardial infraction. Experimental work will be organized in five work packages: WP1. Determination of spatio-temporal profile of ankrd1a expression after injury of adult zebrafish heart and skeletal muscle. WP2. Investigation of ankrd1a function in regeneration of adult zebrafish heart. WP3. Investigation of ankrd1a function in repair of adult zebrafish skeletal muscle. WP4. Identification of ankrd1a downstream targets and associated pathways involved in regeneration of heart and repair of skeletal muscle of adult zebrafish WP5. Testing the mediator role of ankrd1a in the beneficial effect of exercise on regeneration of heart and repair of skeletal muscle.", journal = "Science Fund of the Republic of Serbia, Program IDEAS", title = "Zebrafish ankrd1a as a common player in heart regeneration and skeletal muscle repair - a new prospect for unlocking regenerative potential of human heart (ZEBARR)", url = "https://hdl.handle.net/21.15107/rcub_imagine_2272" }
(2021). Zebrafish ankrd1a as a common player in heart regeneration and skeletal muscle repair - a new prospect for unlocking regenerative potential of human heart (ZEBARR). in Science Fund of the Republic of Serbia, Program IDEAS. https://hdl.handle.net/21.15107/rcub_imagine_2272
Zebrafish ankrd1a as a common player in heart regeneration and skeletal muscle repair - a new prospect for unlocking regenerative potential of human heart (ZEBARR). in Science Fund of the Republic of Serbia, Program IDEAS. 2021;. https://hdl.handle.net/21.15107/rcub_imagine_2272 .
"Zebrafish ankrd1a as a common player in heart regeneration and skeletal muscle repair - a new prospect for unlocking regenerative potential of human heart (ZEBARR)" in Science Fund of the Republic of Serbia, Program IDEAS (2021), https://hdl.handle.net/21.15107/rcub_imagine_2272 .