Toxicity testing of D, L-sulforaphane in a zebrafish model
Autori
Pavić, AleksandarRadaković, Nataša
Srdić-Rajić, Tatjana
Božić, Dragica
Baralić, Katarina
Đukić-Ćosić, Danijela
Konferencijski prilog (Objavljena verzija)
Metapodaci
Prikaz svih podataka o dokumentuApstrakt
Sulforaphane (SFN) belongs to the group of isothiocyanates and is present in many cruciferous plants,
especially broccoli. The positive properties of this bioactive molecule in the form of extracts have been
shown in numerous studies and include antibacterial, cardioprotective, and neuroprotective effects,
antioxidant and immunomodulatory effects, as well as positive effects in various cancers.
However, the potential for harmful effects of SFN has not been sufficiently investigated, particularly for
chemically obtained D,L-sulforaphane. This study aimed to investigate the toxicity of D,L-sulforaphane
on zebrafish (Danio rerio) model. Wild (AB) strain embryos and zebrafish transgenic lines with fluorescently
labeled liver cells (Tg(fabp:EGFP)) and endothelial cells of blood vessels (Tg(fli1:EGFP)) were used,
treated with different concentrations of SFN (1 to 20 μg/mL).
The survival of embryos, developmental toxicity, hepatotoxicity and cardiotoxicity were monitored for
five da...ys. A concentration of 20 μg/mL of D,L-sulforaphane caused the death of all embryos, while the
median lethal concentration (LC50) was found to be 14.2 μg/mL. D,L-sulforaphane exhibited toxic
effects at concentrations higher than 3 μg/mL, primarily on the development of the swim bladder (4
μg/mL), and growth and development of embryos (4.58 μg/mL), while harmful effects on the liver (liver
size and yolk resorption) were observed at a concentration of 10 μg/mL. Effects on the cardiovascular
system were not observed at concentrations from 1 to 10 μg/mL. The investigation of D,L-sulforaphane
on zebrafish embryos showed that harmful effects occur at very low concentrations, indicating the
need for further investigation of toxicological potential of this molecule.
Ključne reči:
mean lethal concentration LC50 / D,L-sulforaphane / toxicity / zebrafishIzvor:
13th International congress of the Serbian society of toxicology and 1. TOXSEE regional conference – present and future of toxicology: challenges and opportunities, 2023, 254-254Izdavač:
- Beograd : Udruženje toksikologa Srbije
Kolekcije
Institucija/grupa
Institut za molekularnu genetiku i genetičko inženjerstvoTY - CONF AU - Pavić, Aleksandar AU - Radaković, Nataša AU - Srdić-Rajić, Tatjana AU - Božić, Dragica AU - Baralić, Katarina AU - Đukić-Ćosić, Danijela PY - 2023 UR - https://imagine.imgge.bg.ac.rs/handle/123456789/2282 AB - Sulforaphane (SFN) belongs to the group of isothiocyanates and is present in many cruciferous plants, especially broccoli. The positive properties of this bioactive molecule in the form of extracts have been shown in numerous studies and include antibacterial, cardioprotective, and neuroprotective effects, antioxidant and immunomodulatory effects, as well as positive effects in various cancers. However, the potential for harmful effects of SFN has not been sufficiently investigated, particularly for chemically obtained D,L-sulforaphane. This study aimed to investigate the toxicity of D,L-sulforaphane on zebrafish (Danio rerio) model. Wild (AB) strain embryos and zebrafish transgenic lines with fluorescently labeled liver cells (Tg(fabp:EGFP)) and endothelial cells of blood vessels (Tg(fli1:EGFP)) were used, treated with different concentrations of SFN (1 to 20 μg/mL). The survival of embryos, developmental toxicity, hepatotoxicity and cardiotoxicity were monitored for five days. A concentration of 20 μg/mL of D,L-sulforaphane caused the death of all embryos, while the median lethal concentration (LC50) was found to be 14.2 μg/mL. D,L-sulforaphane exhibited toxic effects at concentrations higher than 3 μg/mL, primarily on the development of the swim bladder (4 μg/mL), and growth and development of embryos (4.58 μg/mL), while harmful effects on the liver (liver size and yolk resorption) were observed at a concentration of 10 μg/mL. Effects on the cardiovascular system were not observed at concentrations from 1 to 10 μg/mL. The investigation of D,L-sulforaphane on zebrafish embryos showed that harmful effects occur at very low concentrations, indicating the need for further investigation of toxicological potential of this molecule. PB - Beograd : Udruženje toksikologa Srbije C3 - 13th International congress of the Serbian society of toxicology and 1. TOXSEE regional conference – present and future of toxicology: challenges and opportunities T1 - Toxicity testing of D, L-sulforaphane in a zebrafish model EP - 254 SP - 254 UR - https://hdl.handle.net/21.15107/rcub_imagine_2282 ER -
@conference{ author = "Pavić, Aleksandar and Radaković, Nataša and Srdić-Rajić, Tatjana and Božić, Dragica and Baralić, Katarina and Đukić-Ćosić, Danijela", year = "2023", abstract = "Sulforaphane (SFN) belongs to the group of isothiocyanates and is present in many cruciferous plants, especially broccoli. The positive properties of this bioactive molecule in the form of extracts have been shown in numerous studies and include antibacterial, cardioprotective, and neuroprotective effects, antioxidant and immunomodulatory effects, as well as positive effects in various cancers. However, the potential for harmful effects of SFN has not been sufficiently investigated, particularly for chemically obtained D,L-sulforaphane. This study aimed to investigate the toxicity of D,L-sulforaphane on zebrafish (Danio rerio) model. Wild (AB) strain embryos and zebrafish transgenic lines with fluorescently labeled liver cells (Tg(fabp:EGFP)) and endothelial cells of blood vessels (Tg(fli1:EGFP)) were used, treated with different concentrations of SFN (1 to 20 μg/mL). The survival of embryos, developmental toxicity, hepatotoxicity and cardiotoxicity were monitored for five days. A concentration of 20 μg/mL of D,L-sulforaphane caused the death of all embryos, while the median lethal concentration (LC50) was found to be 14.2 μg/mL. D,L-sulforaphane exhibited toxic effects at concentrations higher than 3 μg/mL, primarily on the development of the swim bladder (4 μg/mL), and growth and development of embryos (4.58 μg/mL), while harmful effects on the liver (liver size and yolk resorption) were observed at a concentration of 10 μg/mL. Effects on the cardiovascular system were not observed at concentrations from 1 to 10 μg/mL. The investigation of D,L-sulforaphane on zebrafish embryos showed that harmful effects occur at very low concentrations, indicating the need for further investigation of toxicological potential of this molecule.", publisher = "Beograd : Udruženje toksikologa Srbije", journal = "13th International congress of the Serbian society of toxicology and 1. TOXSEE regional conference – present and future of toxicology: challenges and opportunities", title = "Toxicity testing of D, L-sulforaphane in a zebrafish model", pages = "254-254", url = "https://hdl.handle.net/21.15107/rcub_imagine_2282" }
Pavić, A., Radaković, N., Srdić-Rajić, T., Božić, D., Baralić, K.,& Đukić-Ćosić, D.. (2023). Toxicity testing of D, L-sulforaphane in a zebrafish model. in 13th International congress of the Serbian society of toxicology and 1. TOXSEE regional conference – present and future of toxicology: challenges and opportunities Beograd : Udruženje toksikologa Srbije., 254-254. https://hdl.handle.net/21.15107/rcub_imagine_2282
Pavić A, Radaković N, Srdić-Rajić T, Božić D, Baralić K, Đukić-Ćosić D. Toxicity testing of D, L-sulforaphane in a zebrafish model. in 13th International congress of the Serbian society of toxicology and 1. TOXSEE regional conference – present and future of toxicology: challenges and opportunities. 2023;:254-254. https://hdl.handle.net/21.15107/rcub_imagine_2282 .
Pavić, Aleksandar, Radaković, Nataša, Srdić-Rajić, Tatjana, Božić, Dragica, Baralić, Katarina, Đukić-Ćosić, Danijela, "Toxicity testing of D, L-sulforaphane in a zebrafish model" in 13th International congress of the Serbian society of toxicology and 1. TOXSEE regional conference – present and future of toxicology: challenges and opportunities (2023):254-254, https://hdl.handle.net/21.15107/rcub_imagine_2282 .