Three lines of evidence of the hepatotoxicity young researchers of a mixture containing phthalates and bisphenol a: in silico and two in vivo models
Аутори
Baralić, KatarinaPavić, Aleksandar
Javorac, Dragana
Živančević, Katarina
Božić, Dragica
Radaković, Nataša
Antonijević Miljaković, Evica
Buha Djordjevic, Aleksandra
Ćurčić, Marijana
Bulat, Zorica
Antonijević, Biljana
Đukić-Ćosić, Danijela
Конференцијски прилог (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
The extensive usage of bis(2-ethylhexyl) phthalate (DEHP), dibutyl phthalate (DBP), and bisphenol A
(BPA) creates a lot of opportunities for combined human exposure to these hazardous compounds in
everyday life and a variety of negative outcomes, including hepatotoxicity. In silico research and two
in vivo models were used to investigate the links between a mixture including DEHP, DBP and BPA and
liver injury. Bioinformatic analysis was performed by Comparative Toxicogenomics Database, ShinyGO,
ToppCluster, and Cytoscape. In vivo subacute study included five groups of rats (n = 6): (1) Control: corn
oil, (2) DEHP: 50 mg/kg b.w./day, (3) DBP: 50 mg/kg b.w./day, (4) BPA: 25 mg/kg b.w./day, (5) MIX: DEHP
+ DBP + BPA. Zebrafish embryos were exposed to the investigated substances in multiple dosages, both
alone and in combination (binary and ternary mixtures). Liver damage was linked to 75 DEHP, DBP, and
BPA genes, the majority of which were associated with inflammation/oxidative s...tress, identified as the
most relevant molecular pathways. In rats, significant changes in redox status/bioelements’ level and
pathohistology were more pronounced or evident only in MIX group, suggesting probable additivity.
In a dose-dependent manner, BPA reduced the liver area (LA) index. LA values were decreased by DEHP
(2 μg/mL) and DBP (5 μg/mL), whereas LA index was raised by their higher concentrations. In binary
mixtures, DBP had a lethal effect at the two highest concentrations, whereas BPA directed hepatotoxicity
of the DEHP/DBP/BPA mixture.
Кључне речи:
plasticizers / hepatotoxicity / toxicogenomic data mining / rat / zebrafishИзвор:
13th International congress of the Serbian society of toxicology and 1. TOXSEE regional conference – present and future of toxicology: challenges and opportunities, 2023, 56-56Издавач:
- Beograd : Udruženje toksikologa Srbije
Колекције
Институција/група
Institut za molekularnu genetiku i genetičko inženjerstvoTY - CONF AU - Baralić, Katarina AU - Pavić, Aleksandar AU - Javorac, Dragana AU - Živančević, Katarina AU - Božić, Dragica AU - Radaković, Nataša AU - Antonijević Miljaković, Evica AU - Buha Djordjevic, Aleksandra AU - Ćurčić, Marijana AU - Bulat, Zorica AU - Antonijević, Biljana AU - Đukić-Ćosić, Danijela PY - 2023 UR - https://imagine.imgge.bg.ac.rs/handle/123456789/2283 AB - The extensive usage of bis(2-ethylhexyl) phthalate (DEHP), dibutyl phthalate (DBP), and bisphenol A (BPA) creates a lot of opportunities for combined human exposure to these hazardous compounds in everyday life and a variety of negative outcomes, including hepatotoxicity. In silico research and two in vivo models were used to investigate the links between a mixture including DEHP, DBP and BPA and liver injury. Bioinformatic analysis was performed by Comparative Toxicogenomics Database, ShinyGO, ToppCluster, and Cytoscape. In vivo subacute study included five groups of rats (n = 6): (1) Control: corn oil, (2) DEHP: 50 mg/kg b.w./day, (3) DBP: 50 mg/kg b.w./day, (4) BPA: 25 mg/kg b.w./day, (5) MIX: DEHP + DBP + BPA. Zebrafish embryos were exposed to the investigated substances in multiple dosages, both alone and in combination (binary and ternary mixtures). Liver damage was linked to 75 DEHP, DBP, and BPA genes, the majority of which were associated with inflammation/oxidative stress, identified as the most relevant molecular pathways. In rats, significant changes in redox status/bioelements’ level and pathohistology were more pronounced or evident only in MIX group, suggesting probable additivity. In a dose-dependent manner, BPA reduced the liver area (LA) index. LA values were decreased by DEHP (2 μg/mL) and DBP (5 μg/mL), whereas LA index was raised by their higher concentrations. In binary mixtures, DBP had a lethal effect at the two highest concentrations, whereas BPA directed hepatotoxicity of the DEHP/DBP/BPA mixture. PB - Beograd : Udruženje toksikologa Srbije C3 - 13th International congress of the Serbian society of toxicology and 1. TOXSEE regional conference – present and future of toxicology: challenges and opportunities T1 - Three lines of evidence of the hepatotoxicity young researchers of a mixture containing phthalates and bisphenol a: in silico and two in vivo models EP - 56 SP - 56 UR - https://hdl.handle.net/21.15107/rcub_imagine_2283 ER -
@conference{ author = "Baralić, Katarina and Pavić, Aleksandar and Javorac, Dragana and Živančević, Katarina and Božić, Dragica and Radaković, Nataša and Antonijević Miljaković, Evica and Buha Djordjevic, Aleksandra and Ćurčić, Marijana and Bulat, Zorica and Antonijević, Biljana and Đukić-Ćosić, Danijela", year = "2023", abstract = "The extensive usage of bis(2-ethylhexyl) phthalate (DEHP), dibutyl phthalate (DBP), and bisphenol A (BPA) creates a lot of opportunities for combined human exposure to these hazardous compounds in everyday life and a variety of negative outcomes, including hepatotoxicity. In silico research and two in vivo models were used to investigate the links between a mixture including DEHP, DBP and BPA and liver injury. Bioinformatic analysis was performed by Comparative Toxicogenomics Database, ShinyGO, ToppCluster, and Cytoscape. In vivo subacute study included five groups of rats (n = 6): (1) Control: corn oil, (2) DEHP: 50 mg/kg b.w./day, (3) DBP: 50 mg/kg b.w./day, (4) BPA: 25 mg/kg b.w./day, (5) MIX: DEHP + DBP + BPA. Zebrafish embryos were exposed to the investigated substances in multiple dosages, both alone and in combination (binary and ternary mixtures). Liver damage was linked to 75 DEHP, DBP, and BPA genes, the majority of which were associated with inflammation/oxidative stress, identified as the most relevant molecular pathways. In rats, significant changes in redox status/bioelements’ level and pathohistology were more pronounced or evident only in MIX group, suggesting probable additivity. In a dose-dependent manner, BPA reduced the liver area (LA) index. LA values were decreased by DEHP (2 μg/mL) and DBP (5 μg/mL), whereas LA index was raised by their higher concentrations. In binary mixtures, DBP had a lethal effect at the two highest concentrations, whereas BPA directed hepatotoxicity of the DEHP/DBP/BPA mixture.", publisher = "Beograd : Udruženje toksikologa Srbije", journal = "13th International congress of the Serbian society of toxicology and 1. TOXSEE regional conference – present and future of toxicology: challenges and opportunities", title = "Three lines of evidence of the hepatotoxicity young researchers of a mixture containing phthalates and bisphenol a: in silico and two in vivo models", pages = "56-56", url = "https://hdl.handle.net/21.15107/rcub_imagine_2283" }
Baralić, K., Pavić, A., Javorac, D., Živančević, K., Božić, D., Radaković, N., Antonijević Miljaković, E., Buha Djordjevic, A., Ćurčić, M., Bulat, Z., Antonijević, B.,& Đukić-Ćosić, D.. (2023). Three lines of evidence of the hepatotoxicity young researchers of a mixture containing phthalates and bisphenol a: in silico and two in vivo models. in 13th International congress of the Serbian society of toxicology and 1. TOXSEE regional conference – present and future of toxicology: challenges and opportunities Beograd : Udruženje toksikologa Srbije., 56-56. https://hdl.handle.net/21.15107/rcub_imagine_2283
Baralić K, Pavić A, Javorac D, Živančević K, Božić D, Radaković N, Antonijević Miljaković E, Buha Djordjevic A, Ćurčić M, Bulat Z, Antonijević B, Đukić-Ćosić D. Three lines of evidence of the hepatotoxicity young researchers of a mixture containing phthalates and bisphenol a: in silico and two in vivo models. in 13th International congress of the Serbian society of toxicology and 1. TOXSEE regional conference – present and future of toxicology: challenges and opportunities. 2023;:56-56. https://hdl.handle.net/21.15107/rcub_imagine_2283 .
Baralić, Katarina, Pavić, Aleksandar, Javorac, Dragana, Živančević, Katarina, Božić, Dragica, Radaković, Nataša, Antonijević Miljaković, Evica, Buha Djordjevic, Aleksandra, Ćurčić, Marijana, Bulat, Zorica, Antonijević, Biljana, Đukić-Ćosić, Danijela, "Three lines of evidence of the hepatotoxicity young researchers of a mixture containing phthalates and bisphenol a: in silico and two in vivo models" in 13th International congress of the Serbian society of toxicology and 1. TOXSEE regional conference – present and future of toxicology: challenges and opportunities (2023):56-56, https://hdl.handle.net/21.15107/rcub_imagine_2283 .