Sulforaphane affects morphological changes and cell viability of colon cancer cells: p53 mutation-dependent effect
Autori
Đurić, AnaSrdić-Rajić, Tatjana
Božić, Dragica
Baralić, Katarina
Pavić, Aleksandar
Đukić-Ćosić, Danijela
Konferencijski prilog (Objavljena verzija)
Metapodaci
Prikaz svih podataka o dokumentuApstrakt
Colorectal carcinoma (CRC) is an increasing cause of morbidity and mortality worldwide.
Among biologically active compounds which have been shown to exert cytotoxic effects on human cancer
cells, including CRC, is sulforaphane (SFN), an isothiocyanate compound extracted from cruciferous
vegetables, especially broccoli. This in vitro study aimed to investigate the effect of SFN on the growth
of human CRC cells and its dependency on the expression of p53. Two human CRC cell lines: HT-29
(a p53 mutated line) and HT-116 (a p53 wild-type line) were treated with SFN at concentrations of 0, 4,
8, 16, and 32 μmol/L for 72 h to test its anticancer effect.
Results indicated that SFN induced cell morphological changes and decreased the total number
of viable cells. Treatment of CRC cells with SFN for 72 h resulted in moderate dose-dependent cytotoxicity.
HCT-116 cells, with a p53-wt, were more sensitive (IC50 = 8.41 μM) than p53-mutated HT-29 cells (IC50
= 24.83 μM). These results indic...ate that SFN exhibits the anticancer effect against CRC cells in p53 mutation-
dependent manner (Serbia-China project: 451-03-1203/2021-09).
Ključne reči:
colorectal carcinoma / sulforaphane / cell viability / p53 mutationIzvor:
13th International congress of the Serbian society of toxicology and 1. TOXSEE regional conference – present and future of toxicology: challenges and opportunities, 2023, 180-180Izdavač:
- Beograd : Udruženje toksikologa Srbije
Institucija/grupa
Institut za molekularnu genetiku i genetičko inženjerstvoTY - CONF AU - Đurić, Ana AU - Srdić-Rajić, Tatjana AU - Božić, Dragica AU - Baralić, Katarina AU - Pavić, Aleksandar AU - Đukić-Ćosić, Danijela PY - 2023 UR - https://imagine.imgge.bg.ac.rs/handle/123456789/2284 AB - Colorectal carcinoma (CRC) is an increasing cause of morbidity and mortality worldwide. Among biologically active compounds which have been shown to exert cytotoxic effects on human cancer cells, including CRC, is sulforaphane (SFN), an isothiocyanate compound extracted from cruciferous vegetables, especially broccoli. This in vitro study aimed to investigate the effect of SFN on the growth of human CRC cells and its dependency on the expression of p53. Two human CRC cell lines: HT-29 (a p53 mutated line) and HT-116 (a p53 wild-type line) were treated with SFN at concentrations of 0, 4, 8, 16, and 32 μmol/L for 72 h to test its anticancer effect. Results indicated that SFN induced cell morphological changes and decreased the total number of viable cells. Treatment of CRC cells with SFN for 72 h resulted in moderate dose-dependent cytotoxicity. HCT-116 cells, with a p53-wt, were more sensitive (IC50 = 8.41 μM) than p53-mutated HT-29 cells (IC50 = 24.83 μM). These results indicate that SFN exhibits the anticancer effect against CRC cells in p53 mutation- dependent manner (Serbia-China project: 451-03-1203/2021-09). PB - Beograd : Udruženje toksikologa Srbije C3 - 13th International congress of the Serbian society of toxicology and 1. TOXSEE regional conference – present and future of toxicology: challenges and opportunities T1 - Sulforaphane affects morphological changes and cell viability of colon cancer cells: p53 mutation-dependent effect EP - 180 SP - 180 UR - https://hdl.handle.net/21.15107/rcub_imagine_2284 ER -
@conference{ author = "Đurić, Ana and Srdić-Rajić, Tatjana and Božić, Dragica and Baralić, Katarina and Pavić, Aleksandar and Đukić-Ćosić, Danijela", year = "2023", abstract = "Colorectal carcinoma (CRC) is an increasing cause of morbidity and mortality worldwide. Among biologically active compounds which have been shown to exert cytotoxic effects on human cancer cells, including CRC, is sulforaphane (SFN), an isothiocyanate compound extracted from cruciferous vegetables, especially broccoli. This in vitro study aimed to investigate the effect of SFN on the growth of human CRC cells and its dependency on the expression of p53. Two human CRC cell lines: HT-29 (a p53 mutated line) and HT-116 (a p53 wild-type line) were treated with SFN at concentrations of 0, 4, 8, 16, and 32 μmol/L for 72 h to test its anticancer effect. Results indicated that SFN induced cell morphological changes and decreased the total number of viable cells. Treatment of CRC cells with SFN for 72 h resulted in moderate dose-dependent cytotoxicity. HCT-116 cells, with a p53-wt, were more sensitive (IC50 = 8.41 μM) than p53-mutated HT-29 cells (IC50 = 24.83 μM). These results indicate that SFN exhibits the anticancer effect against CRC cells in p53 mutation- dependent manner (Serbia-China project: 451-03-1203/2021-09).", publisher = "Beograd : Udruženje toksikologa Srbije", journal = "13th International congress of the Serbian society of toxicology and 1. TOXSEE regional conference – present and future of toxicology: challenges and opportunities", title = "Sulforaphane affects morphological changes and cell viability of colon cancer cells: p53 mutation-dependent effect", pages = "180-180", url = "https://hdl.handle.net/21.15107/rcub_imagine_2284" }
Đurić, A., Srdić-Rajić, T., Božić, D., Baralić, K., Pavić, A.,& Đukić-Ćosić, D.. (2023). Sulforaphane affects morphological changes and cell viability of colon cancer cells: p53 mutation-dependent effect. in 13th International congress of the Serbian society of toxicology and 1. TOXSEE regional conference – present and future of toxicology: challenges and opportunities Beograd : Udruženje toksikologa Srbije., 180-180. https://hdl.handle.net/21.15107/rcub_imagine_2284
Đurić A, Srdić-Rajić T, Božić D, Baralić K, Pavić A, Đukić-Ćosić D. Sulforaphane affects morphological changes and cell viability of colon cancer cells: p53 mutation-dependent effect. in 13th International congress of the Serbian society of toxicology and 1. TOXSEE regional conference – present and future of toxicology: challenges and opportunities. 2023;:180-180. https://hdl.handle.net/21.15107/rcub_imagine_2284 .
Đurić, Ana, Srdić-Rajić, Tatjana, Božić, Dragica, Baralić, Katarina, Pavić, Aleksandar, Đukić-Ćosić, Danijela, "Sulforaphane affects morphological changes and cell viability of colon cancer cells: p53 mutation-dependent effect" in 13th International congress of the Serbian society of toxicology and 1. TOXSEE regional conference – present and future of toxicology: challenges and opportunities (2023):180-180, https://hdl.handle.net/21.15107/rcub_imagine_2284 .