DNA/BSA binding affinity of pyocyanin produced by Pseudomonas aeruginosa
Autori
Andrejević, TinaAšanin, Darko
Pantelić, Lena
Pantović, Bojana
Nikodinović-Runić, Jasmina
Glišić, Biljana
Konferencijski prilog (Objavljena verzija)
Metapodaci
Prikaz svih podataka o dokumentuApstrakt
Pyocyanin (PYO) is a green blue pigment that is produced extracellularly by the Gram- negative bacteria Pseudomonas aeruginosa. Its color depends on pH value. It exists in blue zwitterion form at neutral and alkaline conditions, while in an acidic environment, it becomes pink after protonation. PYO has shown the antibacterial activity, as well as the ability to inhibit the growth of fungi like Aspergillus fumigatus and Candida albicans. Moreover, it shows the high cytotoxic effect against the human pancreatic cancer cells by inducing their apoptosis. To evaluate the possible mechanism of antimicrobial activity of PYO, in the present study, we have investigated its interactions with calf thymus DNA (ct-DNA) and bovine serum albumin (BSA) by fluorescence emission spectroscopy. The obtained value of binding constant to BSA is relatively high (KA = 5.3 × 10^6 M^-1 s^-1), showing the ability of PYO to bind to this transport protein. We have also used synchronous fluorescence spectroscopy to... explore the structural changes in BSA in the presence of the studied biopigment. In contrast with the mentioned results for binding to BSA, PYO has shown a low affinity to ct-DNA, what can be seen from the value of its binding constant (KA = 7.8 × 10^3 M^-1 s^-1).
Ključne reči:
BSA interaction / DNA interaction / fluorescence emission spectroscopy / pyocyaninIzvor:
Medical Sciences Forum, 2023, n/aIzdavač:
- MDPI
Finansiranje / projekti:
- BioECOLogics - Value-added biologics through eco-sustainable routes (RS-ScienceFundRS-Ideje-7730810)
- Ministarstvo nauke, tehnološkog razvoja i inovacija Republike Srbije, institucionalno finansiranje - 200042 (Univerzitet u Beogradu, Institut za molekularnu genetiku i genetičko inženjerstvo) (RS-MESTD-inst-2020-200042)
- Ministarstvo nauke, tehnološkog razvoja i inovacija Republike Srbije, institucionalno finansiranje - 200122 (Univerzitet u Kragujevcu, Prirodno-matematički fakultet) (RS-MESTD-inst-2020-200122)
- Ministarstvo nauke, tehnološkog razvoja i inovacija Republike Srbije, institucionalno finansiranje - 200378 (Institut za informacione tehnologije, Kragujevac) (RS-MESTD-inst-2020-200378)
Napomena:
- Presented at the 9th International Electronic Conference on Medicinal Chemistry, 1–30 November 2023; 13 Available online: https://ecmc2023.sciforum.net/
Institucija/grupa
Institut za molekularnu genetiku i genetičko inženjerstvoTY - CONF AU - Andrejević, Tina AU - Ašanin, Darko AU - Pantelić, Lena AU - Pantović, Bojana AU - Nikodinović-Runić, Jasmina AU - Glišić, Biljana PY - 2023 UR - https://imagine.imgge.bg.ac.rs/handle/123456789/2293 AB - Pyocyanin (PYO) is a green blue pigment that is produced extracellularly by the Gram- negative bacteria Pseudomonas aeruginosa. Its color depends on pH value. It exists in blue zwitterion form at neutral and alkaline conditions, while in an acidic environment, it becomes pink after protonation. PYO has shown the antibacterial activity, as well as the ability to inhibit the growth of fungi like Aspergillus fumigatus and Candida albicans. Moreover, it shows the high cytotoxic effect against the human pancreatic cancer cells by inducing their apoptosis. To evaluate the possible mechanism of antimicrobial activity of PYO, in the present study, we have investigated its interactions with calf thymus DNA (ct-DNA) and bovine serum albumin (BSA) by fluorescence emission spectroscopy. The obtained value of binding constant to BSA is relatively high (KA = 5.3 × 10^6 M^-1 s^-1), showing the ability of PYO to bind to this transport protein. We have also used synchronous fluorescence spectroscopy to explore the structural changes in BSA in the presence of the studied biopigment. In contrast with the mentioned results for binding to BSA, PYO has shown a low affinity to ct-DNA, what can be seen from the value of its binding constant (KA = 7.8 × 10^3 M^-1 s^-1). PB - MDPI C3 - Medical Sciences Forum T1 - DNA/BSA binding affinity of pyocyanin produced by Pseudomonas aeruginosa VL - n/a DO - 10.3390/ECMC2023-15654 ER -
@conference{ author = "Andrejević, Tina and Ašanin, Darko and Pantelić, Lena and Pantović, Bojana and Nikodinović-Runić, Jasmina and Glišić, Biljana", year = "2023", abstract = "Pyocyanin (PYO) is a green blue pigment that is produced extracellularly by the Gram- negative bacteria Pseudomonas aeruginosa. Its color depends on pH value. It exists in blue zwitterion form at neutral and alkaline conditions, while in an acidic environment, it becomes pink after protonation. PYO has shown the antibacterial activity, as well as the ability to inhibit the growth of fungi like Aspergillus fumigatus and Candida albicans. Moreover, it shows the high cytotoxic effect against the human pancreatic cancer cells by inducing their apoptosis. To evaluate the possible mechanism of antimicrobial activity of PYO, in the present study, we have investigated its interactions with calf thymus DNA (ct-DNA) and bovine serum albumin (BSA) by fluorescence emission spectroscopy. The obtained value of binding constant to BSA is relatively high (KA = 5.3 × 10^6 M^-1 s^-1), showing the ability of PYO to bind to this transport protein. We have also used synchronous fluorescence spectroscopy to explore the structural changes in BSA in the presence of the studied biopigment. In contrast with the mentioned results for binding to BSA, PYO has shown a low affinity to ct-DNA, what can be seen from the value of its binding constant (KA = 7.8 × 10^3 M^-1 s^-1).", publisher = "MDPI", journal = "Medical Sciences Forum", title = "DNA/BSA binding affinity of pyocyanin produced by Pseudomonas aeruginosa", volume = "n/a", doi = "10.3390/ECMC2023-15654" }
Andrejević, T., Ašanin, D., Pantelić, L., Pantović, B., Nikodinović-Runić, J.,& Glišić, B.. (2023). DNA/BSA binding affinity of pyocyanin produced by Pseudomonas aeruginosa. in Medical Sciences Forum MDPI., n/a. https://doi.org/10.3390/ECMC2023-15654
Andrejević T, Ašanin D, Pantelić L, Pantović B, Nikodinović-Runić J, Glišić B. DNA/BSA binding affinity of pyocyanin produced by Pseudomonas aeruginosa. in Medical Sciences Forum. 2023;n/a. doi:10.3390/ECMC2023-15654 .
Andrejević, Tina, Ašanin, Darko, Pantelić, Lena, Pantović, Bojana, Nikodinović-Runić, Jasmina, Glišić, Biljana, "DNA/BSA binding affinity of pyocyanin produced by Pseudomonas aeruginosa" in Medical Sciences Forum, n/a (2023), https://doi.org/10.3390/ECMC2023-15654 . .