Effect of immunostimulating Limosilactobacillus strain in rats with trinitrobenzenesulfonate (TNBS)-induced colitis
Efekat imunostimulišućeg soja roda Limosilactobacillus kod pacova sa kolitisom izazvanim trinitrobenzensulfonatom (TNBS)
Članak u časopisu (Objavljena verzija)
Metapodaci
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The aim of the study was to test the potential of immunostimulating Limosilactobacillus fermentum BGHI14 strain to reduce the damage of colon tissue in rats with 2,4,6-trinitrobenzenesulfonic acid (TNBS)-induced colitis. Wistar rats were treated with L. fermentum BGHI14 in the regime of preventive, therapeutic and continuous treatment 22 days prior to and/or 7 days after the administration of TNBS. After sacrifice, the colon tissue samples were taken for RNA isolation, gene expression analysis, histopathological analysis, and malondialdehyde measurement. Judging from the body weights, histopathological scores, malondialdehyde levels and transcription of IL-1β and Tight junction protein 1 (Tjp-1) coding genes, preventive and therapeutic treatment proved to be the most protective in the applied conditions. On the other hand, continuous treatment did not affect the intensity of tissue damage. Considering these results, we discussed the possible mechanisms which might stand behind the... protective action of immunostimulating probiotic bacteria in the case of mucosal barrier damage.
Cilj ovog istraživanja je bio da se ispita potencijal imunostimulišućeg soja Limosilactobacillus fermentumBGHI14 da smanji oštećenje tkiva debelog creva kod pacova sa kolitisom izazvanim 2,4,6-trinitrobenzensulfonskom kiselinom (TNBS). Pacovi Wistar soja su tretirani sojem L. fermentumBGHI14 u režimu preventivnog, terapijskog i kontinuiranog tretmana 22 dana pre i/ili 7 dana nakon primene TNBS. Nakon žrtvovanja, uzorci debelog creva su sakupljeni za izolaciju RNK, analizu ekspresije gena, histopatološke analize i merenje malondialdehida. Na osnovu telesnih težina pacova, histopatološih rezultata, nivoa malondialdehida i transkripcije IL-1β citokina i proteina tesnih međućelijskih veza (Tjp-1), preventivni i terapijski tretman su se pokazali kao najefikasniji u primenjenim uslovima. S druge strane, kontinuirano lečenje nije uticalo na intenzitet oštećenja tkiva. Uzimajući u obzir ove rezultate, razmotreni su m...ogući mehanizmi koji stoje iza zaštitnog delovanja imunostimulišućih probiotičkih bakterija u slučaju oštećenja mukozne barijere.
Ključne reči:
colitis / Limosilactobacillus fermentum / probiotic / pro-inflammatory cytokines / lipid peroxidation / kolitis / Limosilactobacillus fermentum / probiotik / pro-inflamatorni citokini / lipidna peroksidacijaIzvor:
Archives of Pharmacy, 2023, 73, Notebook 6, 586-601Izdavač:
- Centre for Evaluation in Education and Science
Finansiranje / projekti:
- Ministarstvo nauke, tehnološkog razvoja i inovacija Republike Srbije, institucionalno finansiranje - 200042 (Univerzitet u Beogradu, Institut za molekularnu genetiku i genetičko inženjerstvo) (RS-MESTD-inst-2020-200042)
URI
https://aseestant.ceon.rs/index.php/arhfarm/article/view/46399https://imagine.imgge.bg.ac.rs/handle/123456789/2298
Institucija/grupa
Institut za molekularnu genetiku i genetičko inženjerstvoTY - JOUR AU - Lukić, Jovanka AU - Strahinić, Ivana AU - Milenković, Marina AU - Begović, Jelena PY - 2023 UR - https://aseestant.ceon.rs/index.php/arhfarm/article/view/46399 UR - https://imagine.imgge.bg.ac.rs/handle/123456789/2298 AB - The aim of the study was to test the potential of immunostimulating Limosilactobacillus fermentum BGHI14 strain to reduce the damage of colon tissue in rats with 2,4,6-trinitrobenzenesulfonic acid (TNBS)-induced colitis. Wistar rats were treated with L. fermentum BGHI14 in the regime of preventive, therapeutic and continuous treatment 22 days prior to and/or 7 days after the administration of TNBS. After sacrifice, the colon tissue samples were taken for RNA isolation, gene expression analysis, histopathological analysis, and malondialdehyde measurement. Judging from the body weights, histopathological scores, malondialdehyde levels and transcription of IL-1β and Tight junction protein 1 (Tjp-1) coding genes, preventive and therapeutic treatment proved to be the most protective in the applied conditions. On the other hand, continuous treatment did not affect the intensity of tissue damage. Considering these results, we discussed the possible mechanisms which might stand behind the protective action of immunostimulating probiotic bacteria in the case of mucosal barrier damage. AB - Cilj ovog istraživanja je bio da se ispita potencijal imunostimulišućeg soja Limosilactobacillus fermentumBGHI14 da smanji oštećenje tkiva debelog creva kod pacova sa kolitisom izazvanim 2,4,6-trinitrobenzensulfonskom kiselinom (TNBS). Pacovi Wistar soja su tretirani sojem L. fermentumBGHI14 u režimu preventivnog, terapijskog i kontinuiranog tretmana 22 dana pre i/ili 7 dana nakon primene TNBS. Nakon žrtvovanja, uzorci debelog creva su sakupljeni za izolaciju RNK, analizu ekspresije gena, histopatološke analize i merenje malondialdehida. Na osnovu telesnih težina pacova, histopatološih rezultata, nivoa malondialdehida i transkripcije IL-1β citokina i proteina tesnih međućelijskih veza (Tjp-1), preventivni i terapijski tretman su se pokazali kao najefikasniji u primenjenim uslovima. S druge strane, kontinuirano lečenje nije uticalo na intenzitet oštećenja tkiva. Uzimajući u obzir ove rezultate, razmotreni su mogući mehanizmi koji stoje iza zaštitnog delovanja imunostimulišućih probiotičkih bakterija u slučaju oštećenja mukozne barijere. PB - Centre for Evaluation in Education and Science T2 - Archives of Pharmacy T1 - Effect of immunostimulating Limosilactobacillus strain in rats with trinitrobenzenesulfonate (TNBS)-induced colitis T1 - Efekat imunostimulišućeg soja roda Limosilactobacillus kod pacova sa kolitisom izazvanim trinitrobenzensulfonatom (TNBS) EP - 601 IS - Notebook 6 SP - 586 VL - 73 DO - 10.5937/arhfarm73-46399 ER -
@article{ author = "Lukić, Jovanka and Strahinić, Ivana and Milenković, Marina and Begović, Jelena", year = "2023", abstract = "The aim of the study was to test the potential of immunostimulating Limosilactobacillus fermentum BGHI14 strain to reduce the damage of colon tissue in rats with 2,4,6-trinitrobenzenesulfonic acid (TNBS)-induced colitis. Wistar rats were treated with L. fermentum BGHI14 in the regime of preventive, therapeutic and continuous treatment 22 days prior to and/or 7 days after the administration of TNBS. After sacrifice, the colon tissue samples were taken for RNA isolation, gene expression analysis, histopathological analysis, and malondialdehyde measurement. Judging from the body weights, histopathological scores, malondialdehyde levels and transcription of IL-1β and Tight junction protein 1 (Tjp-1) coding genes, preventive and therapeutic treatment proved to be the most protective in the applied conditions. On the other hand, continuous treatment did not affect the intensity of tissue damage. Considering these results, we discussed the possible mechanisms which might stand behind the protective action of immunostimulating probiotic bacteria in the case of mucosal barrier damage., Cilj ovog istraživanja je bio da se ispita potencijal imunostimulišućeg soja Limosilactobacillus fermentumBGHI14 da smanji oštećenje tkiva debelog creva kod pacova sa kolitisom izazvanim 2,4,6-trinitrobenzensulfonskom kiselinom (TNBS). Pacovi Wistar soja su tretirani sojem L. fermentumBGHI14 u režimu preventivnog, terapijskog i kontinuiranog tretmana 22 dana pre i/ili 7 dana nakon primene TNBS. Nakon žrtvovanja, uzorci debelog creva su sakupljeni za izolaciju RNK, analizu ekspresije gena, histopatološke analize i merenje malondialdehida. Na osnovu telesnih težina pacova, histopatološih rezultata, nivoa malondialdehida i transkripcije IL-1β citokina i proteina tesnih međućelijskih veza (Tjp-1), preventivni i terapijski tretman su se pokazali kao najefikasniji u primenjenim uslovima. S druge strane, kontinuirano lečenje nije uticalo na intenzitet oštećenja tkiva. Uzimajući u obzir ove rezultate, razmotreni su mogući mehanizmi koji stoje iza zaštitnog delovanja imunostimulišućih probiotičkih bakterija u slučaju oštećenja mukozne barijere.", publisher = "Centre for Evaluation in Education and Science", journal = "Archives of Pharmacy", title = "Effect of immunostimulating Limosilactobacillus strain in rats with trinitrobenzenesulfonate (TNBS)-induced colitis, Efekat imunostimulišućeg soja roda Limosilactobacillus kod pacova sa kolitisom izazvanim trinitrobenzensulfonatom (TNBS)", pages = "601-586", number = "Notebook 6", volume = "73", doi = "10.5937/arhfarm73-46399" }
Lukić, J., Strahinić, I., Milenković, M.,& Begović, J.. (2023). Effect of immunostimulating Limosilactobacillus strain in rats with trinitrobenzenesulfonate (TNBS)-induced colitis. in Archives of Pharmacy Centre for Evaluation in Education and Science., 73(Notebook 6), 586-601. https://doi.org/10.5937/arhfarm73-46399
Lukić J, Strahinić I, Milenković M, Begović J. Effect of immunostimulating Limosilactobacillus strain in rats with trinitrobenzenesulfonate (TNBS)-induced colitis. in Archives of Pharmacy. 2023;73(Notebook 6):586-601. doi:10.5937/arhfarm73-46399 .
Lukić, Jovanka, Strahinić, Ivana, Milenković, Marina, Begović, Jelena, "Effect of immunostimulating Limosilactobacillus strain in rats with trinitrobenzenesulfonate (TNBS)-induced colitis" in Archives of Pharmacy, 73, no. Notebook 6 (2023):586-601, https://doi.org/10.5937/arhfarm73-46399 . .