Molecular characterization of the CmbR activator-binding site in the metC-cysK promoter region in Lactococcus lactis
Abstract
The metC-cysK operon involved in sulphur metabolism in Lactococcus lactis is positively regulated by the LysR-type protein CmbR. Transcription from the metC promoter is activated when concentrations of methionine and cysteine in the growth medium are low. The metC promoter region contains two direct and three inverted repeats. Deletion analysis indicated that direct repeat 2 (DR2) is required for activation of the metC promoter by CmbR. Gel mobility shift assays confirmed that CmbR binds to a 407 bp DNA fragment containing the rnetC promoter. This binding was stimulated by O-acetyl-L-serine. Competition experiments with deletion variants of the metC promoter showed that CmbR binding only occurred with fragments containing an intact DR2, confirming that DR2 is the CmbR binding site within the metC promoter.
Source:
Microbiology-Sgm, 2005, 151, 439-446Publisher:
- Microbiology Soc, London
DOI: 10.1099/mic.0.27411-0
ISSN: 1350-0872
PubMed: 15699193
WoS: 000227031000010
Scopus: 2-s2.0-14044272177
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Institut za molekularnu genetiku i genetičko inženjerstvoTY - JOUR AU - Golić, Nataša AU - Schliekelmann, M AU - Fernandez, M AU - Kleerebezem, M AU - van Kranenburg, R PY - 2005 UR - https://imagine.imgge.bg.ac.rs/handle/123456789/231 AB - The metC-cysK operon involved in sulphur metabolism in Lactococcus lactis is positively regulated by the LysR-type protein CmbR. Transcription from the metC promoter is activated when concentrations of methionine and cysteine in the growth medium are low. The metC promoter region contains two direct and three inverted repeats. Deletion analysis indicated that direct repeat 2 (DR2) is required for activation of the metC promoter by CmbR. Gel mobility shift assays confirmed that CmbR binds to a 407 bp DNA fragment containing the rnetC promoter. This binding was stimulated by O-acetyl-L-serine. Competition experiments with deletion variants of the metC promoter showed that CmbR binding only occurred with fragments containing an intact DR2, confirming that DR2 is the CmbR binding site within the metC promoter. PB - Microbiology Soc, London T2 - Microbiology-Sgm T1 - Molecular characterization of the CmbR activator-binding site in the metC-cysK promoter region in Lactococcus lactis EP - 446 SP - 439 VL - 151 DO - 10.1099/mic.0.27411-0 ER -
@article{ author = "Golić, Nataša and Schliekelmann, M and Fernandez, M and Kleerebezem, M and van Kranenburg, R", year = "2005", abstract = "The metC-cysK operon involved in sulphur metabolism in Lactococcus lactis is positively regulated by the LysR-type protein CmbR. Transcription from the metC promoter is activated when concentrations of methionine and cysteine in the growth medium are low. The metC promoter region contains two direct and three inverted repeats. Deletion analysis indicated that direct repeat 2 (DR2) is required for activation of the metC promoter by CmbR. Gel mobility shift assays confirmed that CmbR binds to a 407 bp DNA fragment containing the rnetC promoter. This binding was stimulated by O-acetyl-L-serine. Competition experiments with deletion variants of the metC promoter showed that CmbR binding only occurred with fragments containing an intact DR2, confirming that DR2 is the CmbR binding site within the metC promoter.", publisher = "Microbiology Soc, London", journal = "Microbiology-Sgm", title = "Molecular characterization of the CmbR activator-binding site in the metC-cysK promoter region in Lactococcus lactis", pages = "446-439", volume = "151", doi = "10.1099/mic.0.27411-0" }
Golić, N., Schliekelmann, M., Fernandez, M., Kleerebezem, M.,& van Kranenburg, R.. (2005). Molecular characterization of the CmbR activator-binding site in the metC-cysK promoter region in Lactococcus lactis. in Microbiology-Sgm Microbiology Soc, London., 151, 439-446. https://doi.org/10.1099/mic.0.27411-0
Golić N, Schliekelmann M, Fernandez M, Kleerebezem M, van Kranenburg R. Molecular characterization of the CmbR activator-binding site in the metC-cysK promoter region in Lactococcus lactis. in Microbiology-Sgm. 2005;151:439-446. doi:10.1099/mic.0.27411-0 .
Golić, Nataša, Schliekelmann, M, Fernandez, M, Kleerebezem, M, van Kranenburg, R, "Molecular characterization of the CmbR activator-binding site in the metC-cysK promoter region in Lactococcus lactis" in Microbiology-Sgm, 151 (2005):439-446, https://doi.org/10.1099/mic.0.27411-0 . .