Proteomic examination of polyester-polyurethane degradation by Streptomyces sp. PU10: Diverting polyurethane intermediates to secondary metabolite production
Autori
Pantelić, BranaSiaperas, Romanos
Budin, Clémence
de Boer, Tjalf
Topakas, Evangelos
Nikodinović-Runić, Jasmina
Članak u časopisu (Objavljena verzija)
Metapodaci
Prikaz svih podataka o dokumentuApstrakt
Global plastic waste accumulation has become omnipresent in public discourse and the focus of scientific research. Ranking as the sixth most produced polymer globally, polyurethanes (PU) significantly contribute to plastic waste and environmental pollution due to the toxicity of their building blocks, such as diisocyanates. In this study, the effects of PU on soil microbial communities over 18 months were monitored revealing that it had marginal effects on microbial diversity. However, Streptomyces sp. PU10, isolated from this PU-contaminated soil, proved exceptional in the degradation of a soluble polyester-PU (Impranil) across a range of temperatures with over 96% degradation of 10 g/L in 48 h. Proteins involved in PU degradation and metabolic changes occurring in this strain with Impranil as the sole carbon source were further investigated employing quantitative proteomics. The proposed degradation mechanism implicated the action of three enzymes: a polyester-degrading esterase, a u...rethane bond-degrading amidase and an oxidoreductase. Furthermore, proteome data revealed that PU degradation intermediates were incorporated into Streptomyces sp. PU10 metabolism via the fatty acid degradation pathway and subsequently channelled to polyketide biosynthesis. Most notably, the production of the tri-pyrrole undecylprodigiosin was confirmed paving the way for establishing PU upcycling strategies to bioactive metabolites using Streptomyces strains.
Izvor:
Microbial Biotechnology, 2024, 17, 3, e14445-Izdavač:
- Wiley
Finansiranje / projekti:
- H2020 Research and Innovation Programme (BioICEP, Grant/Award Number: 870292);
- BioECOLogics - Value-added biologics through eco-sustainable routes (RS-ScienceFundRS-Ideje-7730810)
- Hellenic Foundation for Research and Innovation (H·F.R.I.) (PlastOmics, Grant/ Award Number: 03061)
URI
https://onlinelibrary.wiley.com/doi/abs/10.1111/1751-7915.14445https://imagine.imgge.bg.ac.rs/handle/123456789/2337
Kolekcije
Institucija/grupa
Institut za molekularnu genetiku i genetičko inženjerstvoTY - JOUR AU - Pantelić, Brana AU - Siaperas, Romanos AU - Budin, Clémence AU - de Boer, Tjalf AU - Topakas, Evangelos AU - Nikodinović-Runić, Jasmina PY - 2024 UR - https://onlinelibrary.wiley.com/doi/abs/10.1111/1751-7915.14445 UR - https://imagine.imgge.bg.ac.rs/handle/123456789/2337 AB - Global plastic waste accumulation has become omnipresent in public discourse and the focus of scientific research. Ranking as the sixth most produced polymer globally, polyurethanes (PU) significantly contribute to plastic waste and environmental pollution due to the toxicity of their building blocks, such as diisocyanates. In this study, the effects of PU on soil microbial communities over 18 months were monitored revealing that it had marginal effects on microbial diversity. However, Streptomyces sp. PU10, isolated from this PU-contaminated soil, proved exceptional in the degradation of a soluble polyester-PU (Impranil) across a range of temperatures with over 96% degradation of 10 g/L in 48 h. Proteins involved in PU degradation and metabolic changes occurring in this strain with Impranil as the sole carbon source were further investigated employing quantitative proteomics. The proposed degradation mechanism implicated the action of three enzymes: a polyester-degrading esterase, a urethane bond-degrading amidase and an oxidoreductase. Furthermore, proteome data revealed that PU degradation intermediates were incorporated into Streptomyces sp. PU10 metabolism via the fatty acid degradation pathway and subsequently channelled to polyketide biosynthesis. Most notably, the production of the tri-pyrrole undecylprodigiosin was confirmed paving the way for establishing PU upcycling strategies to bioactive metabolites using Streptomyces strains. PB - Wiley T2 - Microbial Biotechnology T2 - Microbial Biotechnology T1 - Proteomic examination of polyester-polyurethane degradation by Streptomyces sp. PU10: Diverting polyurethane intermediates to secondary metabolite production IS - 3 SP - e14445 VL - 17 DO - 10.1111/1751-7915.14445 ER -
@article{ author = "Pantelić, Brana and Siaperas, Romanos and Budin, Clémence and de Boer, Tjalf and Topakas, Evangelos and Nikodinović-Runić, Jasmina", year = "2024", abstract = "Global plastic waste accumulation has become omnipresent in public discourse and the focus of scientific research. Ranking as the sixth most produced polymer globally, polyurethanes (PU) significantly contribute to plastic waste and environmental pollution due to the toxicity of their building blocks, such as diisocyanates. In this study, the effects of PU on soil microbial communities over 18 months were monitored revealing that it had marginal effects on microbial diversity. However, Streptomyces sp. PU10, isolated from this PU-contaminated soil, proved exceptional in the degradation of a soluble polyester-PU (Impranil) across a range of temperatures with over 96% degradation of 10 g/L in 48 h. Proteins involved in PU degradation and metabolic changes occurring in this strain with Impranil as the sole carbon source were further investigated employing quantitative proteomics. The proposed degradation mechanism implicated the action of three enzymes: a polyester-degrading esterase, a urethane bond-degrading amidase and an oxidoreductase. Furthermore, proteome data revealed that PU degradation intermediates were incorporated into Streptomyces sp. PU10 metabolism via the fatty acid degradation pathway and subsequently channelled to polyketide biosynthesis. Most notably, the production of the tri-pyrrole undecylprodigiosin was confirmed paving the way for establishing PU upcycling strategies to bioactive metabolites using Streptomyces strains.", publisher = "Wiley", journal = "Microbial Biotechnology, Microbial Biotechnology", title = "Proteomic examination of polyester-polyurethane degradation by Streptomyces sp. PU10: Diverting polyurethane intermediates to secondary metabolite production", number = "3", pages = "e14445", volume = "17", doi = "10.1111/1751-7915.14445" }
Pantelić, B., Siaperas, R., Budin, C., de Boer, T., Topakas, E.,& Nikodinović-Runić, J.. (2024). Proteomic examination of polyester-polyurethane degradation by Streptomyces sp. PU10: Diverting polyurethane intermediates to secondary metabolite production. in Microbial Biotechnology Wiley., 17(3), e14445. https://doi.org/10.1111/1751-7915.14445
Pantelić B, Siaperas R, Budin C, de Boer T, Topakas E, Nikodinović-Runić J. Proteomic examination of polyester-polyurethane degradation by Streptomyces sp. PU10: Diverting polyurethane intermediates to secondary metabolite production. in Microbial Biotechnology. 2024;17(3):e14445. doi:10.1111/1751-7915.14445 .
Pantelić, Brana, Siaperas, Romanos, Budin, Clémence, de Boer, Tjalf, Topakas, Evangelos, Nikodinović-Runić, Jasmina, "Proteomic examination of polyester-polyurethane degradation by Streptomyces sp. PU10: Diverting polyurethane intermediates to secondary metabolite production" in Microbial Biotechnology, 17, no. 3 (2024):e14445, https://doi.org/10.1111/1751-7915.14445 . .