SHORT-CHAIN FATTY ACID-PRODUCING FAECALIMONAS SP. NGB245 STRAIN REGULATES THE EXPRESSION OF NEURONAL ACTIVITY-REGULATED GENES AND ATTENUATES THE SYMPTOMS OF EXPERIMENTAL AUTOIMMUNE ENCEPHALOMYELITIS
Аутори
Bisenić, Aleksandar![](/themes/Mirageimagine/images/orcid.png)
Tomić, Sergej
Bekić, Marina
Pavlović, Luka
Dinić, Miroslav
![](/themes/Mirageimagine/images/orcid.png)
Terzić- Vidojević, Amarela
![](/themes/Mirageimagine/images/orcid.png)
Radojević, Dušan
![](/themes/Mirageimagine/images/orcid.png)
Soković Bajić, Svetlana
![](/themes/Mirageimagine/images/orcid.png)
Mitrović, Hristina
![](/themes/Mirageimagine/images/orcid.png)
Jakovljević, Stefan
![](/themes/Mirageimagine/images/orcid.png)
Stevanović, Dušan
Golić, Nataša
![](/themes/Mirageimagine/images/orcid.png)
Đokić, Jelena
![](/themes/Mirageimagine/images/orcid.png)
Остала ауторства
Dimkić, IvicaKekić, Dušan
Конференцијски прилог (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Alterations in gut microbiota and deregulation
of the gut immune system are recognized
as important events in autoimmune diseases.
The knowledge about the important role of anaerobic
gut bacteria that produce short-chain
fatty acids (SCFAs), in the regulation of intestinal
barrier and immune response made a way
for the development of microbiota-based interventions.
Our research aimed to isolate the
strains with the potential to produce SCFAs,
from healthy volunteer fecal material, and to
test their effects on IL-8 production in the culture
of intestinal epithelial cells (Caco2) as an in
vitro system imitating initial intestinal inflammation,
the effects on the expression of neuronal
activity-regulated genes of Caenorhabditis
elegans, and the effect on the development
of experimental autoimmune encephalomyelitis
(EAE), a mouse model of multiple sclerosis.
Three isolated butyric acid (BA)-producing
strains, and three acetic acid (AA)-producing
strains diminished the... production of IL-8 in Caco-
2 cells treated with IL-1β/TNF-α. Further, all
BA-producing strains stimulated the expression
of important neuro-related genes in C. elegans.
Based on the strongest effects in these
assays an isolate identified as Faecalimonas sp.
NGB245 strain was further tested in EAE model.
The oral treatment of EAE-induced mice with
this strain for 16h per day for 15 days resulted
in alleviated daily clinical scores, maximal
clinical scores, and the duration of the illness
in comparison to the effect of media used for
strain cultivation. These results point to the potential
of NGB245 to modify the gut-brain axis
opening the field for future development of microbiota-
based therapy for the diseases associated
with immune response dysfunctions.
Кључне речи:
microbiota-based therapy / short-chain fatty acid (SCFA) / intestinal barrier / immune response / autoimmune diseaseИзвор:
XIII Congress of microbiologists of Serbia: From biotechnology to human and planetary health, 2024, 116-116Издавач:
- Serbian Society for Microbiology
Финансирање / пројекти:
- Министарство науке, технолошког развоја и иновација Републике Србије, институционално финансирање - 200042 (Универзитет у Београду, Институт за молекуларну генетику и генетичко инжењерство) (RS-MESTD-inst-2020-200042)
- Министарство науке, технолошког развоја и иновација Републике Србије, институционално финансирање - 200019 (Универзитет у Београду, Институт за примену нуклеарне енергије - ИНЕП) (RS-MESTD-inst-2020-200019)
- NextGenBiotics - The use of integrative multi-omics approach in cultivation and characterization of gut bacteria related to microbiota-gut-brain axis as a source for Next Generation Probiotics (RS-ScienceFundRS-Ideje-7744507)
Напомена:
- Book of abstract: From biotechnology to human and planetary health XIII congress of microbiologists of Serbia with international participation Mikromed regio 5, ums series 24: 4th – 6th april 2024, Mona Plaza hotel, Belgrade, Serbia
Колекције
Институција/група
Institut za molekularnu genetiku i genetičko inženjerstvoTY - CONF AU - Bisenić, Aleksandar AU - Tomić, Sergej AU - Bekić, Marina AU - Pavlović, Luka AU - Dinić, Miroslav AU - Terzić- Vidojević, Amarela AU - Radojević, Dušan AU - Soković Bajić, Svetlana AU - Mitrović, Hristina AU - Jakovljević, Stefan AU - Stevanović, Dušan AU - Golić, Nataša AU - Đokić, Jelena PY - 2024 UR - https://imagine.imgge.bg.ac.rs/handle/123456789/2374 AB - Alterations in gut microbiota and deregulation of the gut immune system are recognized as important events in autoimmune diseases. The knowledge about the important role of anaerobic gut bacteria that produce short-chain fatty acids (SCFAs), in the regulation of intestinal barrier and immune response made a way for the development of microbiota-based interventions. Our research aimed to isolate the strains with the potential to produce SCFAs, from healthy volunteer fecal material, and to test their effects on IL-8 production in the culture of intestinal epithelial cells (Caco2) as an in vitro system imitating initial intestinal inflammation, the effects on the expression of neuronal activity-regulated genes of Caenorhabditis elegans, and the effect on the development of experimental autoimmune encephalomyelitis (EAE), a mouse model of multiple sclerosis. Three isolated butyric acid (BA)-producing strains, and three acetic acid (AA)-producing strains diminished the production of IL-8 in Caco- 2 cells treated with IL-1β/TNF-α. Further, all BA-producing strains stimulated the expression of important neuro-related genes in C. elegans. Based on the strongest effects in these assays an isolate identified as Faecalimonas sp. NGB245 strain was further tested in EAE model. The oral treatment of EAE-induced mice with this strain for 16h per day for 15 days resulted in alleviated daily clinical scores, maximal clinical scores, and the duration of the illness in comparison to the effect of media used for strain cultivation. These results point to the potential of NGB245 to modify the gut-brain axis opening the field for future development of microbiota- based therapy for the diseases associated with immune response dysfunctions. PB - Serbian Society for Microbiology C3 - XIII Congress of microbiologists of Serbia: From biotechnology to human and planetary health T1 - SHORT-CHAIN FATTY ACID-PRODUCING FAECALIMONAS SP. NGB245 STRAIN REGULATES THE EXPRESSION OF NEURONAL ACTIVITY-REGULATED GENES AND ATTENUATES THE SYMPTOMS OF EXPERIMENTAL AUTOIMMUNE ENCEPHALOMYELITIS EP - 116 SP - 116 UR - https://hdl.handle.net/21.15107/rcub_imagine_2374 ER -
@conference{ author = "Bisenić, Aleksandar and Tomić, Sergej and Bekić, Marina and Pavlović, Luka and Dinić, Miroslav and Terzić- Vidojević, Amarela and Radojević, Dušan and Soković Bajić, Svetlana and Mitrović, Hristina and Jakovljević, Stefan and Stevanović, Dušan and Golić, Nataša and Đokić, Jelena", year = "2024", abstract = "Alterations in gut microbiota and deregulation of the gut immune system are recognized as important events in autoimmune diseases. The knowledge about the important role of anaerobic gut bacteria that produce short-chain fatty acids (SCFAs), in the regulation of intestinal barrier and immune response made a way for the development of microbiota-based interventions. Our research aimed to isolate the strains with the potential to produce SCFAs, from healthy volunteer fecal material, and to test their effects on IL-8 production in the culture of intestinal epithelial cells (Caco2) as an in vitro system imitating initial intestinal inflammation, the effects on the expression of neuronal activity-regulated genes of Caenorhabditis elegans, and the effect on the development of experimental autoimmune encephalomyelitis (EAE), a mouse model of multiple sclerosis. Three isolated butyric acid (BA)-producing strains, and three acetic acid (AA)-producing strains diminished the production of IL-8 in Caco- 2 cells treated with IL-1β/TNF-α. Further, all BA-producing strains stimulated the expression of important neuro-related genes in C. elegans. Based on the strongest effects in these assays an isolate identified as Faecalimonas sp. NGB245 strain was further tested in EAE model. The oral treatment of EAE-induced mice with this strain for 16h per day for 15 days resulted in alleviated daily clinical scores, maximal clinical scores, and the duration of the illness in comparison to the effect of media used for strain cultivation. These results point to the potential of NGB245 to modify the gut-brain axis opening the field for future development of microbiota- based therapy for the diseases associated with immune response dysfunctions.", publisher = "Serbian Society for Microbiology", journal = "XIII Congress of microbiologists of Serbia: From biotechnology to human and planetary health", title = "SHORT-CHAIN FATTY ACID-PRODUCING FAECALIMONAS SP. NGB245 STRAIN REGULATES THE EXPRESSION OF NEURONAL ACTIVITY-REGULATED GENES AND ATTENUATES THE SYMPTOMS OF EXPERIMENTAL AUTOIMMUNE ENCEPHALOMYELITIS", pages = "116-116", url = "https://hdl.handle.net/21.15107/rcub_imagine_2374" }
Bisenić, A., Tomić, S., Bekić, M., Pavlović, L., Dinić, M., Terzić- Vidojević, A., Radojević, D., Soković Bajić, S., Mitrović, H., Jakovljević, S., Stevanović, D., Golić, N.,& Đokić, J.. (2024). SHORT-CHAIN FATTY ACID-PRODUCING FAECALIMONAS SP. NGB245 STRAIN REGULATES THE EXPRESSION OF NEURONAL ACTIVITY-REGULATED GENES AND ATTENUATES THE SYMPTOMS OF EXPERIMENTAL AUTOIMMUNE ENCEPHALOMYELITIS. in XIII Congress of microbiologists of Serbia: From biotechnology to human and planetary health Serbian Society for Microbiology., 116-116. https://hdl.handle.net/21.15107/rcub_imagine_2374
Bisenić A, Tomić S, Bekić M, Pavlović L, Dinić M, Terzić- Vidojević A, Radojević D, Soković Bajić S, Mitrović H, Jakovljević S, Stevanović D, Golić N, Đokić J. SHORT-CHAIN FATTY ACID-PRODUCING FAECALIMONAS SP. NGB245 STRAIN REGULATES THE EXPRESSION OF NEURONAL ACTIVITY-REGULATED GENES AND ATTENUATES THE SYMPTOMS OF EXPERIMENTAL AUTOIMMUNE ENCEPHALOMYELITIS. in XIII Congress of microbiologists of Serbia: From biotechnology to human and planetary health. 2024;:116-116. https://hdl.handle.net/21.15107/rcub_imagine_2374 .
Bisenić, Aleksandar, Tomić, Sergej, Bekić, Marina, Pavlović, Luka, Dinić, Miroslav, Terzić- Vidojević, Amarela, Radojević, Dušan, Soković Bajić, Svetlana, Mitrović, Hristina, Jakovljević, Stefan, Stevanović, Dušan, Golić, Nataša, Đokić, Jelena, "SHORT-CHAIN FATTY ACID-PRODUCING FAECALIMONAS SP. NGB245 STRAIN REGULATES THE EXPRESSION OF NEURONAL ACTIVITY-REGULATED GENES AND ATTENUATES THE SYMPTOMS OF EXPERIMENTAL AUTOIMMUNE ENCEPHALOMYELITIS" in XIII Congress of microbiologists of Serbia: From biotechnology to human and planetary health (2024):116-116, https://hdl.handle.net/21.15107/rcub_imagine_2374 .