HERBAL PRODUCTS AS AN ALTERNATIVE TO ANTIBIOTICS: APPLICATION POSSIBILITIES AND LIMITATIONS
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Antimicrobial resistance (AMR) has developed as
one of the top 10 global public health threats
facing humanity. As the nosocomial bacterial
strains are being increasingly resistant to most
clinically available antibiotics, there is a constant
need for exploration of new substances
that could kill them or inhibit their growth, or
alternatively inhibit some of their essential virulence
factors to counteract the lack of new antibacterials
and the rise of antibiotic resistance,
plants could represent a potential solution.
Plants produce a variety of bioactive secondary
metabolites that could be used to fuel the future
discovery pipeline. Aim of the present study was
to examine inhibitory activity of the supercritical
extract of J. communis L. green pseudofructus
(7SCO2) against the growth, biofilm production
and several virulence factors of significant nosocomial
bacterial pathogens. The extract was
obtained by fractional extraction with supercritical
CO2, and the quali...tative and quantitative
analysis was performed using the GC-FID/MS
method. Clinical isolates of Pseudomonas aeruginosa,Acinetobacter baumannii, Staphylococcus
aureus (methicillin-sensitive-MSSA and methicillin-
resistant - MRSA), Enterococcus faecalis, and
Klebsiella pneumoniae, as well as their antibiotic
resistance profiles, were obtained from the Clinical
Hospital Centre “Dr Dragiša Mišović Dedinje”.
Minimum inhibitory concentrations (MICs) of
the 7SCO2 were determined by broth-microdilution
method. Examination of the anti-adhesive
effect of the extract was carried out using the
spectrophotometric method. The pyocyanin
production of Pseudomonas aeruginosa was determined
by the method described by Rampioni
et al. Most significant findings of this study
are potent antivirulence activity of the 7SCO2
against P. aeruginosa through the inhibition of
pyocyanin production. In addition, the biofilm
production of A. baumannii was inhibited by the
7SCO2 in concentration 50 μg/mL. Finally, notable
antivirulence activity of the 7SCO2 against
E. faecalis and S. aureus was detected, since it
significantly inhibited collagen and laminin adhesion
of these pathogens.
Ključne reči:
Juniperus communis / biofilm / adhesion / pyocyaninIzvor:
XIII Congress of microbiologists of Serbia: From biotechnology to human and planetary health, 2024, 131-131Izdavač:
- Serbian Society for Microbiology
Finansiranje / projekti:
- Ministry of Science, Technological Development and Innovation of the Republic of Serbia (Eureka! network program, project No. E!13632 (GREENTECH))
Napomena:
- Book of abstract: From biotechnology to human and planetary health XIII congress of microbiologists of Serbia with international participation Mikromed regio 5, ums series 24: 4th – 6th april 2024, Mona Plaza hotel, Belgrade, Serbia
Kolekcije
Institucija/grupa
Institut za molekularnu genetiku i genetičko inženjerstvoTY - CONF AU - T. Milenković, Marina AU - Ušjak, Dušan AU - Tadić, Vanja PY - 2024 UR - https://imagine.imgge.bg.ac.rs/handle/123456789/2377 AB - Antimicrobial resistance (AMR) has developed as one of the top 10 global public health threats facing humanity. As the nosocomial bacterial strains are being increasingly resistant to most clinically available antibiotics, there is a constant need for exploration of new substances that could kill them or inhibit their growth, or alternatively inhibit some of their essential virulence factors to counteract the lack of new antibacterials and the rise of antibiotic resistance, plants could represent a potential solution. Plants produce a variety of bioactive secondary metabolites that could be used to fuel the future discovery pipeline. Aim of the present study was to examine inhibitory activity of the supercritical extract of J. communis L. green pseudofructus (7SCO2) against the growth, biofilm production and several virulence factors of significant nosocomial bacterial pathogens. The extract was obtained by fractional extraction with supercritical CO2, and the qualitative and quantitative analysis was performed using the GC-FID/MS method. Clinical isolates of Pseudomonas aeruginosa,Acinetobacter baumannii, Staphylococcus aureus (methicillin-sensitive-MSSA and methicillin- resistant - MRSA), Enterococcus faecalis, and Klebsiella pneumoniae, as well as their antibiotic resistance profiles, were obtained from the Clinical Hospital Centre “Dr Dragiša Mišović Dedinje”. Minimum inhibitory concentrations (MICs) of the 7SCO2 were determined by broth-microdilution method. Examination of the anti-adhesive effect of the extract was carried out using the spectrophotometric method. The pyocyanin production of Pseudomonas aeruginosa was determined by the method described by Rampioni et al. Most significant findings of this study are potent antivirulence activity of the 7SCO2 against P. aeruginosa through the inhibition of pyocyanin production. In addition, the biofilm production of A. baumannii was inhibited by the 7SCO2 in concentration 50 μg/mL. Finally, notable antivirulence activity of the 7SCO2 against E. faecalis and S. aureus was detected, since it significantly inhibited collagen and laminin adhesion of these pathogens. PB - Serbian Society for Microbiology C3 - XIII Congress of microbiologists of Serbia: From biotechnology to human and planetary health T1 - HERBAL PRODUCTS AS AN ALTERNATIVE TO ANTIBIOTICS: APPLICATION POSSIBILITIES AND LIMITATIONS EP - 131 SP - 131 UR - https://hdl.handle.net/21.15107/rcub_imagine_2377 ER -
@conference{ author = "T. Milenković, Marina and Ušjak, Dušan and Tadić, Vanja", year = "2024", abstract = "Antimicrobial resistance (AMR) has developed as one of the top 10 global public health threats facing humanity. As the nosocomial bacterial strains are being increasingly resistant to most clinically available antibiotics, there is a constant need for exploration of new substances that could kill them or inhibit their growth, or alternatively inhibit some of their essential virulence factors to counteract the lack of new antibacterials and the rise of antibiotic resistance, plants could represent a potential solution. Plants produce a variety of bioactive secondary metabolites that could be used to fuel the future discovery pipeline. Aim of the present study was to examine inhibitory activity of the supercritical extract of J. communis L. green pseudofructus (7SCO2) against the growth, biofilm production and several virulence factors of significant nosocomial bacterial pathogens. The extract was obtained by fractional extraction with supercritical CO2, and the qualitative and quantitative analysis was performed using the GC-FID/MS method. Clinical isolates of Pseudomonas aeruginosa,Acinetobacter baumannii, Staphylococcus aureus (methicillin-sensitive-MSSA and methicillin- resistant - MRSA), Enterococcus faecalis, and Klebsiella pneumoniae, as well as their antibiotic resistance profiles, were obtained from the Clinical Hospital Centre “Dr Dragiša Mišović Dedinje”. Minimum inhibitory concentrations (MICs) of the 7SCO2 were determined by broth-microdilution method. Examination of the anti-adhesive effect of the extract was carried out using the spectrophotometric method. The pyocyanin production of Pseudomonas aeruginosa was determined by the method described by Rampioni et al. Most significant findings of this study are potent antivirulence activity of the 7SCO2 against P. aeruginosa through the inhibition of pyocyanin production. In addition, the biofilm production of A. baumannii was inhibited by the 7SCO2 in concentration 50 μg/mL. Finally, notable antivirulence activity of the 7SCO2 against E. faecalis and S. aureus was detected, since it significantly inhibited collagen and laminin adhesion of these pathogens.", publisher = "Serbian Society for Microbiology", journal = "XIII Congress of microbiologists of Serbia: From biotechnology to human and planetary health", title = "HERBAL PRODUCTS AS AN ALTERNATIVE TO ANTIBIOTICS: APPLICATION POSSIBILITIES AND LIMITATIONS", pages = "131-131", url = "https://hdl.handle.net/21.15107/rcub_imagine_2377" }
T. Milenković, M., Ušjak, D.,& Tadić, V.. (2024). HERBAL PRODUCTS AS AN ALTERNATIVE TO ANTIBIOTICS: APPLICATION POSSIBILITIES AND LIMITATIONS. in XIII Congress of microbiologists of Serbia: From biotechnology to human and planetary health Serbian Society for Microbiology., 131-131. https://hdl.handle.net/21.15107/rcub_imagine_2377
T. Milenković M, Ušjak D, Tadić V. HERBAL PRODUCTS AS AN ALTERNATIVE TO ANTIBIOTICS: APPLICATION POSSIBILITIES AND LIMITATIONS. in XIII Congress of microbiologists of Serbia: From biotechnology to human and planetary health. 2024;:131-131. https://hdl.handle.net/21.15107/rcub_imagine_2377 .
T. Milenković, Marina, Ušjak, Dušan, Tadić, Vanja, "HERBAL PRODUCTS AS AN ALTERNATIVE TO ANTIBIOTICS: APPLICATION POSSIBILITIES AND LIMITATIONS" in XIII Congress of microbiologists of Serbia: From biotechnology to human and planetary health (2024):131-131, https://hdl.handle.net/21.15107/rcub_imagine_2377 .