YTNP LACTONASE IMPROVES THE ABILITY OF CAENORHABDITIS ELEGANS TO SURVIVE PSEUDOMONAS AERUGINOSA MMA83 INFECTION
Аутори
Ćurčić, JovanaMalešević, Milka
Dinić, Miroslav
Novović, Katarina
Vasiljević, Zorica
Stanisavljević, Nemanja
Jovčić, Branko
Остала ауторства
Dimkić, IvicaKekić, Dušan
Конференцијски прилог (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Pseudomonas aeruginosa is a Gram-negative
pathogen responsible for frequent hospital-acquired
infections of the bloodstream, the respiratory
tract, and the urinary tract. Quorum
quenching enzymes are recognized as an alternative
antivirulence approach targeting pathogenic
bacteria. The efficacy of YtnP lactonase in
reducing the virulence of P. aeruginosa MMA83
in vivo using Caenorhabditis elegans as a model
system was investigated. The recombinant YtnP
lactonase exhibits no cytotoxicity, demonstrated
by its lack of harmful effects on both the
immortalized human HaCaT cell line and two
strains of C. elegans (AU37 and N2 wild-type). In
a toxin-mediated killing liquid assay, the survival
rates of C. elegans AU37 mutant and N2 wildtype
strains infected with the clinical isolate P.
aeruginosa MMA83 significantly increased when
pre-treated with YtnP lactonase, compared to
untreated controls. Considering that virulence
factors expression is regulated by quorum sensing
(QS...) signaling it is hypothesized that YtnP
lactonase prolongs the life span of C. elegans
by downregulating the QS and expression of
virulence factors of MMA83. The protective effects
of YtnP lactonase against MMA83-induced
pathogenicity in C. elegans, coupled with its absence
of cytotoxicity, position YtnP lactonase as
a promising prophylactic agent with antivirulence
properties.
Кључне речи:
C. elegans / lactonase / P. aeruginosa / infection modelИзвор:
XIII Congress of microbiologists of Serbia: From biotechnology to human and planetary health, 2024, 143-143Издавач:
- Serbian Society for Microbiology
Финансирање / пројекти:
- Министарство науке, технолошког развоја и иновација Републике Србије, институционално финансирање - 200042 (Универзитет у Београду, Институт за молекуларну генетику и генетичко инжењерство) (RS-MESTD-inst-2020-200042)
Напомена:
- Book of abstract: From biotechnology to human and planetary health XIII congress of microbiologists of Serbia with international participation Mikromed regio 5, ums series 24: 4th – 6th april 2024, Mona Plaza hotel, Belgrade, Serbia
Институција/група
Institut za molekularnu genetiku i genetičko inženjerstvoTY - CONF AU - Ćurčić, Jovana AU - Malešević, Milka AU - Dinić, Miroslav AU - Novović, Katarina AU - Vasiljević, Zorica AU - Stanisavljević, Nemanja AU - Jovčić, Branko PY - 2024 UR - https://imagine.imgge.bg.ac.rs/handle/123456789/2380 AB - Pseudomonas aeruginosa is a Gram-negative pathogen responsible for frequent hospital-acquired infections of the bloodstream, the respiratory tract, and the urinary tract. Quorum quenching enzymes are recognized as an alternative antivirulence approach targeting pathogenic bacteria. The efficacy of YtnP lactonase in reducing the virulence of P. aeruginosa MMA83 in vivo using Caenorhabditis elegans as a model system was investigated. The recombinant YtnP lactonase exhibits no cytotoxicity, demonstrated by its lack of harmful effects on both the immortalized human HaCaT cell line and two strains of C. elegans (AU37 and N2 wild-type). In a toxin-mediated killing liquid assay, the survival rates of C. elegans AU37 mutant and N2 wildtype strains infected with the clinical isolate P. aeruginosa MMA83 significantly increased when pre-treated with YtnP lactonase, compared to untreated controls. Considering that virulence factors expression is regulated by quorum sensing (QS) signaling it is hypothesized that YtnP lactonase prolongs the life span of C. elegans by downregulating the QS and expression of virulence factors of MMA83. The protective effects of YtnP lactonase against MMA83-induced pathogenicity in C. elegans, coupled with its absence of cytotoxicity, position YtnP lactonase as a promising prophylactic agent with antivirulence properties. PB - Serbian Society for Microbiology C3 - XIII Congress of microbiologists of Serbia: From biotechnology to human and planetary health T1 - YTNP LACTONASE IMPROVES THE ABILITY OF CAENORHABDITIS ELEGANS TO SURVIVE PSEUDOMONAS AERUGINOSA MMA83 INFECTION EP - 143 SP - 143 UR - https://hdl.handle.net/21.15107/rcub_imagine_2380 ER -
@conference{ author = "Ćurčić, Jovana and Malešević, Milka and Dinić, Miroslav and Novović, Katarina and Vasiljević, Zorica and Stanisavljević, Nemanja and Jovčić, Branko", year = "2024", abstract = "Pseudomonas aeruginosa is a Gram-negative pathogen responsible for frequent hospital-acquired infections of the bloodstream, the respiratory tract, and the urinary tract. Quorum quenching enzymes are recognized as an alternative antivirulence approach targeting pathogenic bacteria. The efficacy of YtnP lactonase in reducing the virulence of P. aeruginosa MMA83 in vivo using Caenorhabditis elegans as a model system was investigated. The recombinant YtnP lactonase exhibits no cytotoxicity, demonstrated by its lack of harmful effects on both the immortalized human HaCaT cell line and two strains of C. elegans (AU37 and N2 wild-type). In a toxin-mediated killing liquid assay, the survival rates of C. elegans AU37 mutant and N2 wildtype strains infected with the clinical isolate P. aeruginosa MMA83 significantly increased when pre-treated with YtnP lactonase, compared to untreated controls. Considering that virulence factors expression is regulated by quorum sensing (QS) signaling it is hypothesized that YtnP lactonase prolongs the life span of C. elegans by downregulating the QS and expression of virulence factors of MMA83. The protective effects of YtnP lactonase against MMA83-induced pathogenicity in C. elegans, coupled with its absence of cytotoxicity, position YtnP lactonase as a promising prophylactic agent with antivirulence properties.", publisher = "Serbian Society for Microbiology", journal = "XIII Congress of microbiologists of Serbia: From biotechnology to human and planetary health", title = "YTNP LACTONASE IMPROVES THE ABILITY OF CAENORHABDITIS ELEGANS TO SURVIVE PSEUDOMONAS AERUGINOSA MMA83 INFECTION", pages = "143-143", url = "https://hdl.handle.net/21.15107/rcub_imagine_2380" }
Ćurčić, J., Malešević, M., Dinić, M., Novović, K., Vasiljević, Z., Stanisavljević, N.,& Jovčić, B.. (2024). YTNP LACTONASE IMPROVES THE ABILITY OF CAENORHABDITIS ELEGANS TO SURVIVE PSEUDOMONAS AERUGINOSA MMA83 INFECTION. in XIII Congress of microbiologists of Serbia: From biotechnology to human and planetary health Serbian Society for Microbiology., 143-143. https://hdl.handle.net/21.15107/rcub_imagine_2380
Ćurčić J, Malešević M, Dinić M, Novović K, Vasiljević Z, Stanisavljević N, Jovčić B. YTNP LACTONASE IMPROVES THE ABILITY OF CAENORHABDITIS ELEGANS TO SURVIVE PSEUDOMONAS AERUGINOSA MMA83 INFECTION. in XIII Congress of microbiologists of Serbia: From biotechnology to human and planetary health. 2024;:143-143. https://hdl.handle.net/21.15107/rcub_imagine_2380 .
Ćurčić, Jovana, Malešević, Milka, Dinić, Miroslav, Novović, Katarina, Vasiljević, Zorica, Stanisavljević, Nemanja, Jovčić, Branko, "YTNP LACTONASE IMPROVES THE ABILITY OF CAENORHABDITIS ELEGANS TO SURVIVE PSEUDOMONAS AERUGINOSA MMA83 INFECTION" in XIII Congress of microbiologists of Serbia: From biotechnology to human and planetary health (2024):143-143, https://hdl.handle.net/21.15107/rcub_imagine_2380 .