MEDICINAL MUSHROOM EXTRACTS ATTENUATE PSEUDOMONAS AERUGINOSA QUORUM SENSING AND VIRULENCE
Autori
Malešević, Milka![](/themes/Mirageimagine/images/orcid.png)
Ćurčić, Jovana
![](/themes/Mirageimagine/images/orcid.png)
Gardijan, Lazar
![](/themes/Mirageimagine/images/orcid.png)
Obradović, Mina
![](/themes/Mirageimagine/images/orcid.png)
Stanisavljević, Nemanja
![](/themes/Mirageimagine/images/orcid.png)
Pantić, Milena
Matijašević, Danka
![](/themes/Mirageimagine/images/orcid.png)
Ostala autorstva
Dimkić, IvicaKekić, Dušan
Konferencijski prilog (Objavljena verzija)
Metapodaci
Prikaz svih podataka o dokumentuApstrakt
Pseudomonas aeruginosa has been recognized as
a priority pathogen by World Health Organization,
due to the emergence of multidrug-resistant
(MDR) strains. Thus, new treatment options
such as antivirulence strategy is urgently needed.
This strategy is based on the disruption of quorum
sensing (QS) activity of this pathogen. The
focus of this research was to explore the anti-QS
activity of four selected medicinal mushrooms
(Lentinula edodes, Cantharellus cibarius, Trametes
versicolor and Pleurotus ostreatus) extracts on
MDR clinical isolate P. aeruginosa MMA83. Another
aim was to check their cytotoxicity on Caenorhabditis
elegans AU37 (glp-4(bn2) I; sec-1(km4).
Among three types of mushroom extracts - hot
water polysaccharide extracts (WPE), hot alkali
polysaccharide extracts (APE) and methanol
extracts (Met), APE extracts downregulated all
tested QS and virulence factors genes of P. aeruginosa
MMA83. The most prominent effect was
observed for C. cibarius APE extract,... lowering
expression from 2-fold (for lasI gene) to 20-fold
for lasB gene. Extracts didn’t show cytotoxic effect
on C. elegans. The efficacy of APE extracts
in lowering the expression of QS and virulence
factors genes of P. aeruginosa MMA83 indicate
that these extracts can reduce pathogenicity of
P. aeruginosa. Also, they possess one of the desirable
biotechnology features – the absence of
cytotoxicity. Anti-QS and antivirulence effect of
APE extracts on P. aeruginosa envisages these extracts
as the promising therapeutic candidates
for the development of next-generation antivirulence
agents.
Ključne reči:
medicinal mushroom extracts / Pseudomonas aeruginosa / quorum sensing inhibition / antivirulenceIzvor:
XIII Congress of microbiologists of Serbia: From biotechnology to human and planetary health, 2024, 145-145Izdavač:
- Serbian Society for Microbiology
Finansiranje / projekti:
- Ministarstvo nauke, tehnološkog razvoja i inovacija Republike Srbije, institucionalno finansiranje - 200042 (Univerzitet u Beogradu, Institut za molekularnu genetiku i genetičko inženjerstvo) (RS-MESTD-inst-2020-200042)
Napomena:
- Book of abstract: From biotechnology to human and planetary health XIII congress of microbiologists of Serbia with international participation Mikromed regio 5, ums series 24: 4th – 6th april 2024, Mona Plaza hotel, Belgrade, Serbia
Institucija/grupa
Institut za molekularnu genetiku i genetičko inženjerstvoTY - CONF AU - Malešević, Milka AU - Ćurčić, Jovana AU - Gardijan, Lazar AU - Obradović, Mina AU - Stanisavljević, Nemanja AU - Pantić, Milena AU - Matijašević, Danka PY - 2024 UR - https://imagine.imgge.bg.ac.rs/handle/123456789/2381 AB - Pseudomonas aeruginosa has been recognized as a priority pathogen by World Health Organization, due to the emergence of multidrug-resistant (MDR) strains. Thus, new treatment options such as antivirulence strategy is urgently needed. This strategy is based on the disruption of quorum sensing (QS) activity of this pathogen. The focus of this research was to explore the anti-QS activity of four selected medicinal mushrooms (Lentinula edodes, Cantharellus cibarius, Trametes versicolor and Pleurotus ostreatus) extracts on MDR clinical isolate P. aeruginosa MMA83. Another aim was to check their cytotoxicity on Caenorhabditis elegans AU37 (glp-4(bn2) I; sec-1(km4). Among three types of mushroom extracts - hot water polysaccharide extracts (WPE), hot alkali polysaccharide extracts (APE) and methanol extracts (Met), APE extracts downregulated all tested QS and virulence factors genes of P. aeruginosa MMA83. The most prominent effect was observed for C. cibarius APE extract, lowering expression from 2-fold (for lasI gene) to 20-fold for lasB gene. Extracts didn’t show cytotoxic effect on C. elegans. The efficacy of APE extracts in lowering the expression of QS and virulence factors genes of P. aeruginosa MMA83 indicate that these extracts can reduce pathogenicity of P. aeruginosa. Also, they possess one of the desirable biotechnology features – the absence of cytotoxicity. Anti-QS and antivirulence effect of APE extracts on P. aeruginosa envisages these extracts as the promising therapeutic candidates for the development of next-generation antivirulence agents. PB - Serbian Society for Microbiology C3 - XIII Congress of microbiologists of Serbia: From biotechnology to human and planetary health T1 - MEDICINAL MUSHROOM EXTRACTS ATTENUATE PSEUDOMONAS AERUGINOSA QUORUM SENSING AND VIRULENCE EP - 145 SP - 145 UR - https://hdl.handle.net/21.15107/rcub_imagine_2381 ER -
@conference{ author = "Malešević, Milka and Ćurčić, Jovana and Gardijan, Lazar and Obradović, Mina and Stanisavljević, Nemanja and Pantić, Milena and Matijašević, Danka", year = "2024", abstract = "Pseudomonas aeruginosa has been recognized as a priority pathogen by World Health Organization, due to the emergence of multidrug-resistant (MDR) strains. Thus, new treatment options such as antivirulence strategy is urgently needed. This strategy is based on the disruption of quorum sensing (QS) activity of this pathogen. The focus of this research was to explore the anti-QS activity of four selected medicinal mushrooms (Lentinula edodes, Cantharellus cibarius, Trametes versicolor and Pleurotus ostreatus) extracts on MDR clinical isolate P. aeruginosa MMA83. Another aim was to check their cytotoxicity on Caenorhabditis elegans AU37 (glp-4(bn2) I; sec-1(km4). Among three types of mushroom extracts - hot water polysaccharide extracts (WPE), hot alkali polysaccharide extracts (APE) and methanol extracts (Met), APE extracts downregulated all tested QS and virulence factors genes of P. aeruginosa MMA83. The most prominent effect was observed for C. cibarius APE extract, lowering expression from 2-fold (for lasI gene) to 20-fold for lasB gene. Extracts didn’t show cytotoxic effect on C. elegans. The efficacy of APE extracts in lowering the expression of QS and virulence factors genes of P. aeruginosa MMA83 indicate that these extracts can reduce pathogenicity of P. aeruginosa. Also, they possess one of the desirable biotechnology features – the absence of cytotoxicity. Anti-QS and antivirulence effect of APE extracts on P. aeruginosa envisages these extracts as the promising therapeutic candidates for the development of next-generation antivirulence agents.", publisher = "Serbian Society for Microbiology", journal = "XIII Congress of microbiologists of Serbia: From biotechnology to human and planetary health", title = "MEDICINAL MUSHROOM EXTRACTS ATTENUATE PSEUDOMONAS AERUGINOSA QUORUM SENSING AND VIRULENCE", pages = "145-145", url = "https://hdl.handle.net/21.15107/rcub_imagine_2381" }
Malešević, M., Ćurčić, J., Gardijan, L., Obradović, M., Stanisavljević, N., Pantić, M.,& Matijašević, D.. (2024). MEDICINAL MUSHROOM EXTRACTS ATTENUATE PSEUDOMONAS AERUGINOSA QUORUM SENSING AND VIRULENCE. in XIII Congress of microbiologists of Serbia: From biotechnology to human and planetary health Serbian Society for Microbiology., 145-145. https://hdl.handle.net/21.15107/rcub_imagine_2381
Malešević M, Ćurčić J, Gardijan L, Obradović M, Stanisavljević N, Pantić M, Matijašević D. MEDICINAL MUSHROOM EXTRACTS ATTENUATE PSEUDOMONAS AERUGINOSA QUORUM SENSING AND VIRULENCE. in XIII Congress of microbiologists of Serbia: From biotechnology to human and planetary health. 2024;:145-145. https://hdl.handle.net/21.15107/rcub_imagine_2381 .
Malešević, Milka, Ćurčić, Jovana, Gardijan, Lazar, Obradović, Mina, Stanisavljević, Nemanja, Pantić, Milena, Matijašević, Danka, "MEDICINAL MUSHROOM EXTRACTS ATTENUATE PSEUDOMONAS AERUGINOSA QUORUM SENSING AND VIRULENCE" in XIII Congress of microbiologists of Serbia: From biotechnology to human and planetary health (2024):145-145, https://hdl.handle.net/21.15107/rcub_imagine_2381 .