mir-219 as a potential radiosensitier in glioblastoma cells
Нема приказа
Аутори
Petrović, MajaStanojković, Tatjana
Drakulić, Danijela
Stanisavljević Ninković, Danijela
Остала ауторства
Bračun, InesKrstanović, Fran Krstanović
Medved, Magdalena
Materljan, Jelena
Конференцијски прилог (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Glioblastoma (GBM) is the most aggressive type of primary malignant brain tumor, and
is characterized by an extremely poor therapeutic response and overall survival.
Adjuvant radiotherapy remains the standard treatment after surgical resection.
Resistance to radiotherapy is frequently observed in patients with GBM, which limits
treatment efficacy. Finding drugs or molecules that can overcome this restrain is crucial
for finding novel more effective glioblastoma treatments. MicroRNAs (miRNAs) are a
class of small non-coding RNAs that are important regulatory molecules that can control
GBM radiosensitivity by affecting radiation-related signal transduction pathways, such
as proliferation, migration, senescence, and cell cycle regulation. Evolutionary
conserved miR-219 has specific expression in brain and it can act as tumour suppressor
in GBM. The main goal of this study is to analyze the effects of modulation of miR-219
expression on radiosensitivity of GBM cells.
First, in ...silico analyses show that the expression of miR-219 is downregulated in GBM
tumours and that many of the target genes of this miRNA have a critical role in the
modulation of key cellular pathways that mediate response to radiation. Additionally, our
results show increased expression of miR-219 upon irradiation of GBM cells. To further
investigate the function of mir-219 in GBM radiosensitivity, a stable miR-219-
overexpressed LN229 and U87 cell line was constructed using lentivirus transduction. In
addition, we analyze the effect of miR-219 overexpression on proliferation and
senescence of GBM cells. The results of this research will contribute to better
understanding how miR-219 affect radiosensitivity of GBM cells and may lead to
improvement of radiotherapy of GBM tumours making progress in treatment of this
aggressive and one of the deadliest forms of brain cancer.
Извор:
2nd Biomedicine and Health PhD Students Congress, 2024Издавач:
- University of Rijeka, Faculty of Medicine
Напомена:
- The 2 nd Biomedicine and HealthPhD Students Congress Faculty of Medicine University of Rijeka, Croatia 16.-18.5.2024.
URI
https://medri.uniri.hr/en/scientific-and-professional-meetings/science-and-us/2nd-biomedicine-and-health-phd-students-congress/https://imagine.imgge.bg.ac.rs/handle/123456789/2394
Колекције
Институција/група
Institut za molekularnu genetiku i genetičko inženjerstvoTY - CONF AU - Petrović, Maja AU - Stanojković, Tatjana AU - Drakulić, Danijela AU - Stanisavljević Ninković, Danijela PY - 2024 UR - https://medri.uniri.hr/en/scientific-and-professional-meetings/science-and-us/2nd-biomedicine-and-health-phd-students-congress/ UR - https://imagine.imgge.bg.ac.rs/handle/123456789/2394 AB - Glioblastoma (GBM) is the most aggressive type of primary malignant brain tumor, and is characterized by an extremely poor therapeutic response and overall survival. Adjuvant radiotherapy remains the standard treatment after surgical resection. Resistance to radiotherapy is frequently observed in patients with GBM, which limits treatment efficacy. Finding drugs or molecules that can overcome this restrain is crucial for finding novel more effective glioblastoma treatments. MicroRNAs (miRNAs) are a class of small non-coding RNAs that are important regulatory molecules that can control GBM radiosensitivity by affecting radiation-related signal transduction pathways, such as proliferation, migration, senescence, and cell cycle regulation. Evolutionary conserved miR-219 has specific expression in brain and it can act as tumour suppressor in GBM. The main goal of this study is to analyze the effects of modulation of miR-219 expression on radiosensitivity of GBM cells. First, in silico analyses show that the expression of miR-219 is downregulated in GBM tumours and that many of the target genes of this miRNA have a critical role in the modulation of key cellular pathways that mediate response to radiation. Additionally, our results show increased expression of miR-219 upon irradiation of GBM cells. To further investigate the function of mir-219 in GBM radiosensitivity, a stable miR-219- overexpressed LN229 and U87 cell line was constructed using lentivirus transduction. In addition, we analyze the effect of miR-219 overexpression on proliferation and senescence of GBM cells. The results of this research will contribute to better understanding how miR-219 affect radiosensitivity of GBM cells and may lead to improvement of radiotherapy of GBM tumours making progress in treatment of this aggressive and one of the deadliest forms of brain cancer. PB - University of Rijeka, Faculty of Medicine C3 - 2nd Biomedicine and Health PhD Students Congress T1 - mir-219 as a potential radiosensitier in glioblastoma cells UR - https://hdl.handle.net/21.15107/rcub_imagine_2394 ER -
@conference{ author = "Petrović, Maja and Stanojković, Tatjana and Drakulić, Danijela and Stanisavljević Ninković, Danijela", year = "2024", abstract = "Glioblastoma (GBM) is the most aggressive type of primary malignant brain tumor, and is characterized by an extremely poor therapeutic response and overall survival. Adjuvant radiotherapy remains the standard treatment after surgical resection. Resistance to radiotherapy is frequently observed in patients with GBM, which limits treatment efficacy. Finding drugs or molecules that can overcome this restrain is crucial for finding novel more effective glioblastoma treatments. MicroRNAs (miRNAs) are a class of small non-coding RNAs that are important regulatory molecules that can control GBM radiosensitivity by affecting radiation-related signal transduction pathways, such as proliferation, migration, senescence, and cell cycle regulation. Evolutionary conserved miR-219 has specific expression in brain and it can act as tumour suppressor in GBM. The main goal of this study is to analyze the effects of modulation of miR-219 expression on radiosensitivity of GBM cells. First, in silico analyses show that the expression of miR-219 is downregulated in GBM tumours and that many of the target genes of this miRNA have a critical role in the modulation of key cellular pathways that mediate response to radiation. Additionally, our results show increased expression of miR-219 upon irradiation of GBM cells. To further investigate the function of mir-219 in GBM radiosensitivity, a stable miR-219- overexpressed LN229 and U87 cell line was constructed using lentivirus transduction. In addition, we analyze the effect of miR-219 overexpression on proliferation and senescence of GBM cells. The results of this research will contribute to better understanding how miR-219 affect radiosensitivity of GBM cells and may lead to improvement of radiotherapy of GBM tumours making progress in treatment of this aggressive and one of the deadliest forms of brain cancer.", publisher = "University of Rijeka, Faculty of Medicine", journal = "2nd Biomedicine and Health PhD Students Congress", title = "mir-219 as a potential radiosensitier in glioblastoma cells", url = "https://hdl.handle.net/21.15107/rcub_imagine_2394" }
Petrović, M., Stanojković, T., Drakulić, D.,& Stanisavljević Ninković, D.. (2024). mir-219 as a potential radiosensitier in glioblastoma cells. in 2nd Biomedicine and Health PhD Students Congress University of Rijeka, Faculty of Medicine.. https://hdl.handle.net/21.15107/rcub_imagine_2394
Petrović M, Stanojković T, Drakulić D, Stanisavljević Ninković D. mir-219 as a potential radiosensitier in glioblastoma cells. in 2nd Biomedicine and Health PhD Students Congress. 2024;. https://hdl.handle.net/21.15107/rcub_imagine_2394 .
Petrović, Maja, Stanojković, Tatjana, Drakulić, Danijela, Stanisavljević Ninković, Danijela, "mir-219 as a potential radiosensitier in glioblastoma cells" in 2nd Biomedicine and Health PhD Students Congress (2024), https://hdl.handle.net/21.15107/rcub_imagine_2394 .