dc.contributor | Morić, Ivana | |
dc.contributor | Đorđević, Valentina | |
dc.creator | Milić, Dejana | |
dc.creator | Balliau, Thierry | |
dc.creator | Davanture, Marlène | |
dc.creator | Blein-Nicolas, Melisande | |
dc.creator | Vidović, Marija | |
dc.date.accessioned | 2024-07-24T21:56:28Z | |
dc.date.available | 2024-07-24T21:56:28Z | |
dc.date.issued | 2024 | |
dc.identifier.isbn | 978-86-82679-16-5 | |
dc.identifier.uri | www.belbi.bg.ac.rs | |
dc.identifier.uri | https://imagine.imgge.bg.ac.rs/handle/123456789/2455 | |
dc.description.abstract | Variegated Pelargonium zonale plants present excellent model for studying metabolite
fluxes and photosynthesis-related processes between the photosynthetically active
(green, vG) and inactive (white, vW) tissues within the same leaf under the same
microenvironmental conditions. We aimed to investigate the proteomic differences
between these two metabolically contrasting tissue types, as well as between vG and
plain morphs (G) to gain more insight into the evolutionary benefits of variegation.
Label-free proteomics was performed using liquid chromatography coupled to tandem
mass spectrometry. The data were searched against the newly expanded RNAseq
database for P. zonale leaves. Analysis was done with X!Tandem, using 10 ppm precursor
mass precision and fragment 0.02 Th mass tolerance. The identified proteins were filtered
using X!TandemPipeline, requiring at least two peptides with E-values lower than 0.01
and a protein E-value<10-5. Peptide ions, and their parental proteins, were quantified by
integrating signal intensities from extracted ion currents (XIC) using MassChroQ software.
A total of 2707 protein groups were identified. After removing dubious data and peptides
with missing values, we obtained 2009 protein group. We annotated 564 and 79
differentially abundant proteins in vG vs. vW and vG vs. G, respectively. The differentially
abundant proteins were mapped into metabolic pathways using the modified MapMan
3.6.0RC1 software. Proteins related to photosynthesis and carbohydrate metabolism
were more abundant in vG compared with vW, while proteins related to oxidative stress
and protein degradation were more abundant in vW than in vG. Compared to vG, G tissue
contained more proteins involved in energy production and protein synthesis. Briefly,
this study has paved the way to uncover the evolutionary advantages of the variegated
phenotype. | sr |
dc.language.iso | en | sr |
dc.publisher | Belgrade : Institute of Molecular Genetics and Genetic Engineering | sr |
dc.relation | info:eu-repo/grantAgreement/MESTD/inst-2020/200042/RS// | sr |
dc.relation | Bilateral project with Republic of France (no. 337-00-93/2023-05/3). | sr |
dc.rights | openAccess | sr |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/4.0/ | |
dc.source | 5th Belgrade Bioinformatics Conference | sr |
dc.subject | differential proteomic analysis | sr |
dc.subject | variegated Pelargonium zonale | sr |
dc.subject | metabolic pathways | sr |
dc.title | Label-Free Quantitative Proteomics of Pelargonium zonale: Tissue-Specific Differences | sr |
dc.type | conferenceObject | sr |
dc.rights.license | BY-NC-ND | sr |
dc.rights.holder | © 2024 Institute of Molecular Genetics and Genetic Engineering, University of Belgrade | sr |
dc.citation.epage | 52 | |
dc.citation.spage | 52 | |
dc.description.other | Book of abstracts: 5th Belgrade Bioinformatics Conference, Serbia, Belgrade,17-20 june 2024. | sr |
dc.identifier.fulltext | https://imagine.imgge.bg.ac.rs/bitstream/id/845703/BelBi2024-Book-of-Abstracts_1-16,68,141-142.pdf | |
dc.identifier.rcub | https://hdl.handle.net/21.15107/rcub_imagine_2455 | |
dc.type.version | publishedVersion | sr |