Echinococcus spp. circulating in livestock in Serbia
Abstract
The echinococcosis case burden in the human population of Serbia
is among the highest in Southeastern Europe, but comprehensive
data on the circulating tapeworm species is scarce. To
gain insight into the population genetics of Echinococcus spp. in
Serbia, the project WORM_PROFILER will collect samples from
livestock intended for human consumption at major abattoirs,
slaughterhouses and homesteads over the next two years. During
the first two months of sampling, 22 livers from pigs (n=15), lambs
(n=5) and calves (n=2) with clearly identifiable cysts and/or cystlike
formations as well as scar tissue were collected from major
abattoirs around the country. The cyst content and/or affected area
was sampled by direct isolation using pipette tips and/or excision
of the tissue and analyzed microscopically, followed by quick
DNA extraction by boiling in 0.02 M NaOH for 15 min and amplification
of the cox1 gene using primers which can differentiate
E. granulosus, E. multiloculari...s and E. canadensis via conventional
PCR. Several large cysts were present only in the liver of one boar.
Tapeworm larvae were microscopically confirmed in the cysts’
content and later identified by PCR as E. granulosus. PCR analysis
additionally revealed the presence of E. canadensis gDNA in a
tissue sample from another pig from a different farm. Although
these results indicate that most carcasses flagged for conditional
use based on liver examination by abattoir veterinarians are
not infected by Echinococcus spp., they nonetheless suggest that
pigs may play an important role in its transmission in Serbia.Sequencing of the cox1 and nad1 genes from the samples in which
Echinococcus spp. gDNA was detected and is currently underway.
This research was supported by the Science Fund of the Republic of
Serbia, #10841, Worm Profiler: Surveillance and population genetics
of Echinococcus in Serbia - WORM_PROFILER.
Keywords:
Echinococcus spp. / livestock / liver / population geneticsSource:
Annals of Parasitology, 2024, 70, Supplement 1Publisher:
- POLISH PARASITOLOGICAL SOCIETY
Note:
- Book of Abstracts:The XIV European Multicolloquium of Parasitology Wrocław, Poland August 26–30, 2024
Collections
Institution/Community
Institut za molekularnu genetiku i genetičko inženjerstvoTY - CONF AU - Breka, Katarina AU - Betić, Nikola AU - Karanović, Jelena AU - Klun, Ivana AU - Uzelac, Aleksandra PY - 2024 UR - https://imagine.imgge.bg.ac.rs/handle/123456789/2501 AB - The echinococcosis case burden in the human population of Serbia is among the highest in Southeastern Europe, but comprehensive data on the circulating tapeworm species is scarce. To gain insight into the population genetics of Echinococcus spp. in Serbia, the project WORM_PROFILER will collect samples from livestock intended for human consumption at major abattoirs, slaughterhouses and homesteads over the next two years. During the first two months of sampling, 22 livers from pigs (n=15), lambs (n=5) and calves (n=2) with clearly identifiable cysts and/or cystlike formations as well as scar tissue were collected from major abattoirs around the country. The cyst content and/or affected area was sampled by direct isolation using pipette tips and/or excision of the tissue and analyzed microscopically, followed by quick DNA extraction by boiling in 0.02 M NaOH for 15 min and amplification of the cox1 gene using primers which can differentiate E. granulosus, E. multilocularis and E. canadensis via conventional PCR. Several large cysts were present only in the liver of one boar. Tapeworm larvae were microscopically confirmed in the cysts’ content and later identified by PCR as E. granulosus. PCR analysis additionally revealed the presence of E. canadensis gDNA in a tissue sample from another pig from a different farm. Although these results indicate that most carcasses flagged for conditional use based on liver examination by abattoir veterinarians are not infected by Echinococcus spp., they nonetheless suggest that pigs may play an important role in its transmission in Serbia.Sequencing of the cox1 and nad1 genes from the samples in which Echinococcus spp. gDNA was detected and is currently underway. This research was supported by the Science Fund of the Republic of Serbia, #10841, Worm Profiler: Surveillance and population genetics of Echinococcus in Serbia - WORM_PROFILER. PB - POLISH PARASITOLOGICAL SOCIETY C3 - Annals of Parasitology T1 - Echinococcus spp. circulating in livestock in Serbia IS - Supplement 1 VL - 70 UR - https://hdl.handle.net/21.15107/rcub_imagine_2501 ER -
@conference{ author = "Breka, Katarina and Betić, Nikola and Karanović, Jelena and Klun, Ivana and Uzelac, Aleksandra", year = "2024", abstract = "The echinococcosis case burden in the human population of Serbia is among the highest in Southeastern Europe, but comprehensive data on the circulating tapeworm species is scarce. To gain insight into the population genetics of Echinococcus spp. in Serbia, the project WORM_PROFILER will collect samples from livestock intended for human consumption at major abattoirs, slaughterhouses and homesteads over the next two years. During the first two months of sampling, 22 livers from pigs (n=15), lambs (n=5) and calves (n=2) with clearly identifiable cysts and/or cystlike formations as well as scar tissue were collected from major abattoirs around the country. The cyst content and/or affected area was sampled by direct isolation using pipette tips and/or excision of the tissue and analyzed microscopically, followed by quick DNA extraction by boiling in 0.02 M NaOH for 15 min and amplification of the cox1 gene using primers which can differentiate E. granulosus, E. multilocularis and E. canadensis via conventional PCR. Several large cysts were present only in the liver of one boar. Tapeworm larvae were microscopically confirmed in the cysts’ content and later identified by PCR as E. granulosus. PCR analysis additionally revealed the presence of E. canadensis gDNA in a tissue sample from another pig from a different farm. Although these results indicate that most carcasses flagged for conditional use based on liver examination by abattoir veterinarians are not infected by Echinococcus spp., they nonetheless suggest that pigs may play an important role in its transmission in Serbia.Sequencing of the cox1 and nad1 genes from the samples in which Echinococcus spp. gDNA was detected and is currently underway. This research was supported by the Science Fund of the Republic of Serbia, #10841, Worm Profiler: Surveillance and population genetics of Echinococcus in Serbia - WORM_PROFILER.", publisher = "POLISH PARASITOLOGICAL SOCIETY", journal = "Annals of Parasitology", title = "Echinococcus spp. circulating in livestock in Serbia", number = "Supplement 1", volume = "70", url = "https://hdl.handle.net/21.15107/rcub_imagine_2501" }
Breka, K., Betić, N., Karanović, J., Klun, I.,& Uzelac, A.. (2024). Echinococcus spp. circulating in livestock in Serbia. in Annals of Parasitology POLISH PARASITOLOGICAL SOCIETY., 70(Supplement 1). https://hdl.handle.net/21.15107/rcub_imagine_2501
Breka K, Betić N, Karanović J, Klun I, Uzelac A. Echinococcus spp. circulating in livestock in Serbia. in Annals of Parasitology. 2024;70(Supplement 1). https://hdl.handle.net/21.15107/rcub_imagine_2501 .
Breka, Katarina, Betić, Nikola, Karanović, Jelena, Klun, Ivana, Uzelac, Aleksandra, "Echinococcus spp. circulating in livestock in Serbia" in Annals of Parasitology, 70, no. Supplement 1 (2024), https://hdl.handle.net/21.15107/rcub_imagine_2501 .