Izolacija i karakterizacija bioaktivnih sekundarnih metabolita iz odabranih sojeva roda Streptomyces
Isolation and characterization of bioactive secondary metabolites from selected Streptomyces strains
2012
Аутори
Stanković, NadaОстала ауторства
Vasiljević, BrankaFira, Đorđe
Vasiljević, Branka
Fira, Đorđe
Nikodinović-Runić, Jasmina
Докторска теза (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Actinomycetes are Gram-positive soil bacteria known as producers of secondary bioactive metabolites. In the search for new bioactive compounds collection of actinomycetes from the Laboratory for Microbial Molecular Genetics and Ecology (Institute of Molecular Genetic and Genetic Engineering, University of Belgrade) was screened according to several criteria - bioactivity, the presence of the genes for polyketide synthase (PKS), pigmentation, and crude culture extracts absorption profile in ultraviolet and visible (UV/Vis) spectra. Based on these we have isolated three strains NP10, JS520, and Streptomyces durmitotensis, previously renewed for didehydroroflamicoin (DDHR) production. Isolates NP10 and JS520 were identified to belong to the genus Streptomyces. Streptomyces sp. NP10 accumulated branched chain fatty acids, predominately isopalmitic, and cyclic dipeptides consisting of proline in combination with valine, isoleucine or alanine, that have a stimulating effect on the trophoblas...t cell line in culture at low concentrations (1 ng/ml and 1 mg/ml) and cytotoxic effect at high concentrations (1 mg/ml). Streptomyces sp. JS520 was determined to be exceptionally good producer of pigment undecylprodigiosine (UP) with antibacterial, antifungal, antioxidant and UV protective activities showing cytotoxic effects on trophoblast cell line in culture at high concentrations (1 ng/ml). Cytotoxicity of polyene macrolide antibiotic DDHR from S. durmitotensis was confirmed and it’s antifungal effects were shown. PKS cluster responsible for DDHR synthesis was subcloned in the cosmid library and partially sequenced. Optimization of culture conditions resulted in 2.12 times increased production of UP, and 1.1 times increased production of DDHR.
Кључне речи:
undecilprodigiozin / Streptomyces / Streptomyces durmitorensis / sekundarni metaboliti / PKS / pigmenti / izoalkanske masne kiseline / bioaktivna jedinjenja / biciklični peptidi / undecylprodigiosine / Streptomyces / Streptomyces durmitotensis / secondary metabolites / PKS / pigments / iso-branched fatty acids / bioactive compounds / bicyclic peptidesИзвор:
2012Издавач:
- Univerzitet u Beogradu, Biološki fakultet
Финансирање / пројекти:
- Изучавање микробиолошког диверзитета и карактеризација корисних срединских микроорганизама (RS-MESTD-Basic Research (BR or ON)-173048)
URI
http://eteze.bg.ac.rs/application/showtheses?thesesId=87https://nardus.mpn.gov.rs/handle/123456789/2068
https://fedorabg.bg.ac.rs/fedora/get/o:3492/bdef:Content/download
http://vbs.rs/scripts/cobiss?command=DISPLAY&base=70036&RID=1024553906
https://imagine.imgge.bg.ac.rs/handle/123456789/26
Колекције
Институција/група
Institut za molekularnu genetiku i genetičko inženjerstvoTY - THES AU - Stanković, Nada PY - 2012 UR - http://eteze.bg.ac.rs/application/showtheses?thesesId=87 UR - https://nardus.mpn.gov.rs/handle/123456789/2068 UR - https://fedorabg.bg.ac.rs/fedora/get/o:3492/bdef:Content/download UR - http://vbs.rs/scripts/cobiss?command=DISPLAY&base=70036&RID=1024553906 UR - https://imagine.imgge.bg.ac.rs/handle/123456789/26 AB - Actinomycetes are Gram-positive soil bacteria known as producers of secondary bioactive metabolites. In the search for new bioactive compounds collection of actinomycetes from the Laboratory for Microbial Molecular Genetics and Ecology (Institute of Molecular Genetic and Genetic Engineering, University of Belgrade) was screened according to several criteria - bioactivity, the presence of the genes for polyketide synthase (PKS), pigmentation, and crude culture extracts absorption profile in ultraviolet and visible (UV/Vis) spectra. Based on these we have isolated three strains NP10, JS520, and Streptomyces durmitotensis, previously renewed for didehydroroflamicoin (DDHR) production. Isolates NP10 and JS520 were identified to belong to the genus Streptomyces. Streptomyces sp. NP10 accumulated branched chain fatty acids, predominately isopalmitic, and cyclic dipeptides consisting of proline in combination with valine, isoleucine or alanine, that have a stimulating effect on the trophoblast cell line in culture at low concentrations (1 ng/ml and 1 mg/ml) and cytotoxic effect at high concentrations (1 mg/ml). Streptomyces sp. JS520 was determined to be exceptionally good producer of pigment undecylprodigiosine (UP) with antibacterial, antifungal, antioxidant and UV protective activities showing cytotoxic effects on trophoblast cell line in culture at high concentrations (1 ng/ml). Cytotoxicity of polyene macrolide antibiotic DDHR from S. durmitotensis was confirmed and it’s antifungal effects were shown. PKS cluster responsible for DDHR synthesis was subcloned in the cosmid library and partially sequenced. Optimization of culture conditions resulted in 2.12 times increased production of UP, and 1.1 times increased production of DDHR. PB - Univerzitet u Beogradu, Biološki fakultet T1 - Izolacija i karakterizacija bioaktivnih sekundarnih metabolita iz odabranih sojeva roda Streptomyces T1 - Isolation and characterization of bioactive secondary metabolites from selected Streptomyces strains UR - https://hdl.handle.net/21.15107/rcub_nardus_2068 ER -
@phdthesis{ author = "Stanković, Nada", year = "2012", abstract = "Actinomycetes are Gram-positive soil bacteria known as producers of secondary bioactive metabolites. In the search for new bioactive compounds collection of actinomycetes from the Laboratory for Microbial Molecular Genetics and Ecology (Institute of Molecular Genetic and Genetic Engineering, University of Belgrade) was screened according to several criteria - bioactivity, the presence of the genes for polyketide synthase (PKS), pigmentation, and crude culture extracts absorption profile in ultraviolet and visible (UV/Vis) spectra. Based on these we have isolated three strains NP10, JS520, and Streptomyces durmitotensis, previously renewed for didehydroroflamicoin (DDHR) production. Isolates NP10 and JS520 were identified to belong to the genus Streptomyces. Streptomyces sp. NP10 accumulated branched chain fatty acids, predominately isopalmitic, and cyclic dipeptides consisting of proline in combination with valine, isoleucine or alanine, that have a stimulating effect on the trophoblast cell line in culture at low concentrations (1 ng/ml and 1 mg/ml) and cytotoxic effect at high concentrations (1 mg/ml). Streptomyces sp. JS520 was determined to be exceptionally good producer of pigment undecylprodigiosine (UP) with antibacterial, antifungal, antioxidant and UV protective activities showing cytotoxic effects on trophoblast cell line in culture at high concentrations (1 ng/ml). Cytotoxicity of polyene macrolide antibiotic DDHR from S. durmitotensis was confirmed and it’s antifungal effects were shown. PKS cluster responsible for DDHR synthesis was subcloned in the cosmid library and partially sequenced. Optimization of culture conditions resulted in 2.12 times increased production of UP, and 1.1 times increased production of DDHR.", publisher = "Univerzitet u Beogradu, Biološki fakultet", title = "Izolacija i karakterizacija bioaktivnih sekundarnih metabolita iz odabranih sojeva roda Streptomyces, Isolation and characterization of bioactive secondary metabolites from selected Streptomyces strains", url = "https://hdl.handle.net/21.15107/rcub_nardus_2068" }
Stanković, N.. (2012). Izolacija i karakterizacija bioaktivnih sekundarnih metabolita iz odabranih sojeva roda Streptomyces. Univerzitet u Beogradu, Biološki fakultet.. https://hdl.handle.net/21.15107/rcub_nardus_2068
Stanković N. Izolacija i karakterizacija bioaktivnih sekundarnih metabolita iz odabranih sojeva roda Streptomyces. 2012;. https://hdl.handle.net/21.15107/rcub_nardus_2068 .
Stanković, Nada, "Izolacija i karakterizacija bioaktivnih sekundarnih metabolita iz odabranih sojeva roda Streptomyces" (2012), https://hdl.handle.net/21.15107/rcub_nardus_2068 .
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