Elemental analysis of the Mycobacterium avium phagosome in Balb/c mouse macrophages
Abstract
Using a hard X-ray microprobe, we showed recently that in unstimulated peritoneal macrophages from C57BL/6 mice, the phagosome of pathogenic mycobacteria (Mycobacterium tuberculosis and Mycobacterium avium) can accumulate iron. We expanded our studies to the M. avium infection of peritoneal macrophages of Balb/c mice that show a similar degree of M. tuberculosis and M. avium-related chronic disease, but a higher susceptibility towards other intracellular pathogens such as Listeria monocytogenes, Leishmania major, or Brucella abortus as compared to C57BL/6 mice. Similar to C57BL/6 macrophages, the iron concentration in Balb/c macrophages increased significantly after 24 h of infection. A significant increase of the chlorine and potassium concentrations was observed in the Balb/c phagosomes between 1 and 24 h, in contrast with macrophages from C57BL/6 mice. The absolute elemental concentrations of calcium and zinc were higher in the mycobacterial phagosomes of Balb/c mice. We hypothesize... that a potassium channel is abundant in the phagosome in macrophages that may be related to microbiocidal killing, similar to the requirement of potassium channels for microbiocidal function in neutrophils.
Keywords:
Potassium channel / Phagosomes / Mycobacteria / Metals / Killing / Iron / Inbred mice / ChlorineSource:
Biochemical and Biophysical Research Communications, 2006, 344, 4, 1346-1351Funding / projects:
- We thank Dan Legnini for the development of high-precision specimen mounts. This work was supported by Grant # ROI-AI 47010 of the National Institutes of Health and by the US Department of Energy, Office of Energy Sciences, under Contract No. W-31-109-Eng-38.
DOI: 10.1016/j.bbrc.2006.04.048
ISSN: 0006-291X
PubMed: 16650826
Scopus: 2-s2.0-33646197903
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Institution/Community
Institut za molekularnu genetiku i genetičko inženjerstvoTY - JOUR AU - Wagner, D. AU - Maser, J. AU - Morić, Ivana AU - Vogt, S. AU - Kern, W.V. AU - Bermudez, L.E. PY - 2006 UR - https://imagine.imgge.bg.ac.rs/handle/123456789/261 AB - Using a hard X-ray microprobe, we showed recently that in unstimulated peritoneal macrophages from C57BL/6 mice, the phagosome of pathogenic mycobacteria (Mycobacterium tuberculosis and Mycobacterium avium) can accumulate iron. We expanded our studies to the M. avium infection of peritoneal macrophages of Balb/c mice that show a similar degree of M. tuberculosis and M. avium-related chronic disease, but a higher susceptibility towards other intracellular pathogens such as Listeria monocytogenes, Leishmania major, or Brucella abortus as compared to C57BL/6 mice. Similar to C57BL/6 macrophages, the iron concentration in Balb/c macrophages increased significantly after 24 h of infection. A significant increase of the chlorine and potassium concentrations was observed in the Balb/c phagosomes between 1 and 24 h, in contrast with macrophages from C57BL/6 mice. The absolute elemental concentrations of calcium and zinc were higher in the mycobacterial phagosomes of Balb/c mice. We hypothesize that a potassium channel is abundant in the phagosome in macrophages that may be related to microbiocidal killing, similar to the requirement of potassium channels for microbiocidal function in neutrophils. T2 - Biochemical and Biophysical Research Communications T1 - Elemental analysis of the Mycobacterium avium phagosome in Balb/c mouse macrophages EP - 1351 IS - 4 SP - 1346 VL - 344 DO - 10.1016/j.bbrc.2006.04.048 ER -
@article{ author = "Wagner, D. and Maser, J. and Morić, Ivana and Vogt, S. and Kern, W.V. and Bermudez, L.E.", year = "2006", abstract = "Using a hard X-ray microprobe, we showed recently that in unstimulated peritoneal macrophages from C57BL/6 mice, the phagosome of pathogenic mycobacteria (Mycobacterium tuberculosis and Mycobacterium avium) can accumulate iron. We expanded our studies to the M. avium infection of peritoneal macrophages of Balb/c mice that show a similar degree of M. tuberculosis and M. avium-related chronic disease, but a higher susceptibility towards other intracellular pathogens such as Listeria monocytogenes, Leishmania major, or Brucella abortus as compared to C57BL/6 mice. Similar to C57BL/6 macrophages, the iron concentration in Balb/c macrophages increased significantly after 24 h of infection. A significant increase of the chlorine and potassium concentrations was observed in the Balb/c phagosomes between 1 and 24 h, in contrast with macrophages from C57BL/6 mice. The absolute elemental concentrations of calcium and zinc were higher in the mycobacterial phagosomes of Balb/c mice. We hypothesize that a potassium channel is abundant in the phagosome in macrophages that may be related to microbiocidal killing, similar to the requirement of potassium channels for microbiocidal function in neutrophils.", journal = "Biochemical and Biophysical Research Communications", title = "Elemental analysis of the Mycobacterium avium phagosome in Balb/c mouse macrophages", pages = "1351-1346", number = "4", volume = "344", doi = "10.1016/j.bbrc.2006.04.048" }
Wagner, D., Maser, J., Morić, I., Vogt, S., Kern, W.V.,& Bermudez, L.E.. (2006). Elemental analysis of the Mycobacterium avium phagosome in Balb/c mouse macrophages. in Biochemical and Biophysical Research Communications, 344(4), 1346-1351. https://doi.org/10.1016/j.bbrc.2006.04.048
Wagner D, Maser J, Morić I, Vogt S, Kern W, Bermudez L. Elemental analysis of the Mycobacterium avium phagosome in Balb/c mouse macrophages. in Biochemical and Biophysical Research Communications. 2006;344(4):1346-1351. doi:10.1016/j.bbrc.2006.04.048 .
Wagner, D., Maser, J., Morić, Ivana, Vogt, S., Kern, W.V., Bermudez, L.E., "Elemental analysis of the Mycobacterium avium phagosome in Balb/c mouse macrophages" in Biochemical and Biophysical Research Communications, 344, no. 4 (2006):1346-1351, https://doi.org/10.1016/j.bbrc.2006.04.048 . .