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Elemental analysis of the Mycobacterium avium phagosome in Balb/c mouse macrophages
dc.creator | Wagner, D. | |
dc.creator | Maser, J. | |
dc.creator | Morić, Ivana | |
dc.creator | Vogt, S. | |
dc.creator | Kern, W.V. | |
dc.creator | Bermudez, L.E. | |
dc.date.accessioned | 2022-11-15T13:44:38Z | |
dc.date.available | 2022-11-15T13:44:38Z | |
dc.date.issued | 2006 | |
dc.identifier.issn | 0006-291X | |
dc.identifier.uri | https://imagine.imgge.bg.ac.rs/handle/123456789/261 | |
dc.description.abstract | Using a hard X-ray microprobe, we showed recently that in unstimulated peritoneal macrophages from C57BL/6 mice, the phagosome of pathogenic mycobacteria (Mycobacterium tuberculosis and Mycobacterium avium) can accumulate iron. We expanded our studies to the M. avium infection of peritoneal macrophages of Balb/c mice that show a similar degree of M. tuberculosis and M. avium-related chronic disease, but a higher susceptibility towards other intracellular pathogens such as Listeria monocytogenes, Leishmania major, or Brucella abortus as compared to C57BL/6 mice. Similar to C57BL/6 macrophages, the iron concentration in Balb/c macrophages increased significantly after 24 h of infection. A significant increase of the chlorine and potassium concentrations was observed in the Balb/c phagosomes between 1 and 24 h, in contrast with macrophages from C57BL/6 mice. The absolute elemental concentrations of calcium and zinc were higher in the mycobacterial phagosomes of Balb/c mice. We hypothesize that a potassium channel is abundant in the phagosome in macrophages that may be related to microbiocidal killing, similar to the requirement of potassium channels for microbiocidal function in neutrophils. | en |
dc.relation | We thank Dan Legnini for the development of high-precision specimen mounts. This work was supported by Grant # ROI-AI 47010 of the National Institutes of Health and by the US Department of Energy, Office of Energy Sciences, under Contract No. W-31-109-Eng-38. | |
dc.rights | restrictedAccess | |
dc.source | Biochemical and Biophysical Research Communications | |
dc.subject | Potassium channel | en |
dc.subject | Phagosomes | en |
dc.subject | Mycobacteria | en |
dc.subject | Metals | en |
dc.subject | Killing | en |
dc.subject | Iron | en |
dc.subject | Inbred mice | en |
dc.subject | Chlorine | en |
dc.title | Elemental analysis of the Mycobacterium avium phagosome in Balb/c mouse macrophages | en |
dc.type | article | |
dc.rights.license | ARR | |
dc.citation.epage | 1351 | |
dc.citation.issue | 4 | |
dc.citation.other | 344(4): 1346-1351 | |
dc.citation.rank | M22 | |
dc.citation.spage | 1346 | |
dc.citation.volume | 344 | |
dc.identifier.doi | 10.1016/j.bbrc.2006.04.048 | |
dc.identifier.pmid | 16650826 | |
dc.identifier.scopus | 2-s2.0-33646197903 | |
dc.type.version | publishedVersion |