Molecular analysis of Y chromosome microdeletions in idiopathic cases of male infertility in Serbia.
Апстракт
The aim of this study was to detect frequency of microdeletions of Y chromosome in idiopathic cases of male infertility in Serbian population. Patients were subjected to detailed clinical, endocrinological and cytogenetic examinations. Ninety patients with normal cytogenetic findings with azoospermia and severe oligozoospermia were included in the study. In these patients microdeletion analysis was performed by multiplex polymerase chain reaction (PCR) method on DNA extracted from peripheral blood. In each case 6 markers in azoospermia factor (AZF) regions were tested: sY84, sY86 (AZFa); sY127, sY134 (AZFb); sY254, sY255 (AZFc). Deletions on Y chromosome were detected in 14 of 90 cases (15.6%), 9 with azoospermia and 5 with severe oligozoospermia. Of total number of 17 deletions, 11 (64.7%) were detected in AZFc region, 3 (17.6%) in AZFa region and 3 (17.6%) in AZFb region. Microdeletions in AZF region of Y chromosome, especially AZFc microdeletions, represent common genetic cause of i...diopathic azoospermia and severe oligozoospremia in Serbian infertile men. Therefore, testing for Y chromosome microdeletions should be considered as an important element in diagnosis and genetic counseling of infertile men in Serbia and decisions regarding the assisted reproduction should be made based on the presence and type of AZF microdeletions.
Извор:
Genetika, 2007, 43, 6, 850-854Институција/група
Institut za molekularnu genetiku i genetičko inženjerstvoTY - JOUR AU - Ristanović, M. AU - Bunjevacki, V. AU - Tulić, C. AU - Novaković, I. AU - Nikolić, Aleksandra PY - 2007 UR - https://imagine.imgge.bg.ac.rs/handle/123456789/290 AB - The aim of this study was to detect frequency of microdeletions of Y chromosome in idiopathic cases of male infertility in Serbian population. Patients were subjected to detailed clinical, endocrinological and cytogenetic examinations. Ninety patients with normal cytogenetic findings with azoospermia and severe oligozoospermia were included in the study. In these patients microdeletion analysis was performed by multiplex polymerase chain reaction (PCR) method on DNA extracted from peripheral blood. In each case 6 markers in azoospermia factor (AZF) regions were tested: sY84, sY86 (AZFa); sY127, sY134 (AZFb); sY254, sY255 (AZFc). Deletions on Y chromosome were detected in 14 of 90 cases (15.6%), 9 with azoospermia and 5 with severe oligozoospermia. Of total number of 17 deletions, 11 (64.7%) were detected in AZFc region, 3 (17.6%) in AZFa region and 3 (17.6%) in AZFb region. Microdeletions in AZF region of Y chromosome, especially AZFc microdeletions, represent common genetic cause of idiopathic azoospermia and severe oligozoospremia in Serbian infertile men. Therefore, testing for Y chromosome microdeletions should be considered as an important element in diagnosis and genetic counseling of infertile men in Serbia and decisions regarding the assisted reproduction should be made based on the presence and type of AZF microdeletions. T2 - Genetika T1 - Molecular analysis of Y chromosome microdeletions in idiopathic cases of male infertility in Serbia. EP - 854 IS - 6 SP - 850 VL - 43 UR - https://hdl.handle.net/21.15107/rcub_imagine_290 ER -
@article{ author = "Ristanović, M. and Bunjevacki, V. and Tulić, C. and Novaković, I. and Nikolić, Aleksandra", year = "2007", abstract = "The aim of this study was to detect frequency of microdeletions of Y chromosome in idiopathic cases of male infertility in Serbian population. Patients were subjected to detailed clinical, endocrinological and cytogenetic examinations. Ninety patients with normal cytogenetic findings with azoospermia and severe oligozoospermia were included in the study. In these patients microdeletion analysis was performed by multiplex polymerase chain reaction (PCR) method on DNA extracted from peripheral blood. In each case 6 markers in azoospermia factor (AZF) regions were tested: sY84, sY86 (AZFa); sY127, sY134 (AZFb); sY254, sY255 (AZFc). Deletions on Y chromosome were detected in 14 of 90 cases (15.6%), 9 with azoospermia and 5 with severe oligozoospermia. Of total number of 17 deletions, 11 (64.7%) were detected in AZFc region, 3 (17.6%) in AZFa region and 3 (17.6%) in AZFb region. Microdeletions in AZF region of Y chromosome, especially AZFc microdeletions, represent common genetic cause of idiopathic azoospermia and severe oligozoospremia in Serbian infertile men. Therefore, testing for Y chromosome microdeletions should be considered as an important element in diagnosis and genetic counseling of infertile men in Serbia and decisions regarding the assisted reproduction should be made based on the presence and type of AZF microdeletions.", journal = "Genetika", title = "Molecular analysis of Y chromosome microdeletions in idiopathic cases of male infertility in Serbia.", pages = "854-850", number = "6", volume = "43", url = "https://hdl.handle.net/21.15107/rcub_imagine_290" }
Ristanović, M., Bunjevacki, V., Tulić, C., Novaković, I.,& Nikolić, A.. (2007). Molecular analysis of Y chromosome microdeletions in idiopathic cases of male infertility in Serbia.. in Genetika, 43(6), 850-854. https://hdl.handle.net/21.15107/rcub_imagine_290
Ristanović M, Bunjevacki V, Tulić C, Novaković I, Nikolić A. Molecular analysis of Y chromosome microdeletions in idiopathic cases of male infertility in Serbia.. in Genetika. 2007;43(6):850-854. https://hdl.handle.net/21.15107/rcub_imagine_290 .
Ristanović, M., Bunjevacki, V., Tulić, C., Novaković, I., Nikolić, Aleksandra, "Molecular analysis of Y chromosome microdeletions in idiopathic cases of male infertility in Serbia." in Genetika, 43, no. 6 (2007):850-854, https://hdl.handle.net/21.15107/rcub_imagine_290 .