Formation of noncanonical DNA structures mediated by human ORC4, a protein component of the origin recognition complex
Само за регистроване кориснике
2008
Чланак у часопису (Објављена верзија)
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Метаподаци
Приказ свих података о документуАпстракт
Many genomic sequences, DNA replication origins included, contain specific structural motifs prone to alternative base pairing. Structural rearrangements of DNA require specific environmental conditions and could be favored by chemical agents or proteins. To improve our understanding of alternative conformations of origins and the manner in which they form, we have investigated the effect of DNA-binding, AAA+ protein human ORC4 on single-stranded origin DNA or various oligonucleotides. Here we demonstrate that human ORC4 stimulated formation of inter- and intramolecular T center dot A center dot T triplexes and created novel structures, such as homoadenine duplexes. Adenine-based structures were held together by Hoogsteen hydrogen bonds, as demonstrated on 7-deaza-dAMP- or dAMP-containing substrates, and characterized by increased thermal stability. Adenine pairing occurred only in the presence of human ORC4, in a neutral buffer supplemented with ATP and Mg2+ ions. The protein mutant t...hat could not bind ATP was inactive in this reaction. Since the action of human ORC4 could be biologically important, its potential impact on DNA replication is discussed.
Извор:
Biochemistry, 2008, 47, 33, 8760-8767Издавач:
- Amer Chemical Soc, Washington
Финансирање / пројекти:
- Структурални елементи генома у модулацији фенотипа (RS-MESTD-MPN2006-2010-143051)
- International Centre for Genetic Engineering and Biotechnology, Italy [YUG 03-01]
DOI: 10.1021/bi800684f
ISSN: 0006-2960
PubMed: 18652488
WoS: 000258400100032
Scopus: 2-s2.0-49749152737
Институција/група
Institut za molekularnu genetiku i genetičko inženjerstvoTY - JOUR AU - Stefanović, Dragana AU - Kušić-Tišma, Jelena AU - Divac Rankov, Aleksandra AU - Tomić, Branko PY - 2008 UR - https://imagine.imgge.bg.ac.rs/handle/123456789/306 AB - Many genomic sequences, DNA replication origins included, contain specific structural motifs prone to alternative base pairing. Structural rearrangements of DNA require specific environmental conditions and could be favored by chemical agents or proteins. To improve our understanding of alternative conformations of origins and the manner in which they form, we have investigated the effect of DNA-binding, AAA+ protein human ORC4 on single-stranded origin DNA or various oligonucleotides. Here we demonstrate that human ORC4 stimulated formation of inter- and intramolecular T center dot A center dot T triplexes and created novel structures, such as homoadenine duplexes. Adenine-based structures were held together by Hoogsteen hydrogen bonds, as demonstrated on 7-deaza-dAMP- or dAMP-containing substrates, and characterized by increased thermal stability. Adenine pairing occurred only in the presence of human ORC4, in a neutral buffer supplemented with ATP and Mg2+ ions. The protein mutant that could not bind ATP was inactive in this reaction. Since the action of human ORC4 could be biologically important, its potential impact on DNA replication is discussed. PB - Amer Chemical Soc, Washington T2 - Biochemistry T1 - Formation of noncanonical DNA structures mediated by human ORC4, a protein component of the origin recognition complex EP - 8767 IS - 33 SP - 8760 VL - 47 DO - 10.1021/bi800684f ER -
@article{ author = "Stefanović, Dragana and Kušić-Tišma, Jelena and Divac Rankov, Aleksandra and Tomić, Branko", year = "2008", abstract = "Many genomic sequences, DNA replication origins included, contain specific structural motifs prone to alternative base pairing. Structural rearrangements of DNA require specific environmental conditions and could be favored by chemical agents or proteins. To improve our understanding of alternative conformations of origins and the manner in which they form, we have investigated the effect of DNA-binding, AAA+ protein human ORC4 on single-stranded origin DNA or various oligonucleotides. Here we demonstrate that human ORC4 stimulated formation of inter- and intramolecular T center dot A center dot T triplexes and created novel structures, such as homoadenine duplexes. Adenine-based structures were held together by Hoogsteen hydrogen bonds, as demonstrated on 7-deaza-dAMP- or dAMP-containing substrates, and characterized by increased thermal stability. Adenine pairing occurred only in the presence of human ORC4, in a neutral buffer supplemented with ATP and Mg2+ ions. The protein mutant that could not bind ATP was inactive in this reaction. Since the action of human ORC4 could be biologically important, its potential impact on DNA replication is discussed.", publisher = "Amer Chemical Soc, Washington", journal = "Biochemistry", title = "Formation of noncanonical DNA structures mediated by human ORC4, a protein component of the origin recognition complex", pages = "8767-8760", number = "33", volume = "47", doi = "10.1021/bi800684f" }
Stefanović, D., Kušić-Tišma, J., Divac Rankov, A.,& Tomić, B.. (2008). Formation of noncanonical DNA structures mediated by human ORC4, a protein component of the origin recognition complex. in Biochemistry Amer Chemical Soc, Washington., 47(33), 8760-8767. https://doi.org/10.1021/bi800684f
Stefanović D, Kušić-Tišma J, Divac Rankov A, Tomić B. Formation of noncanonical DNA structures mediated by human ORC4, a protein component of the origin recognition complex. in Biochemistry. 2008;47(33):8760-8767. doi:10.1021/bi800684f .
Stefanović, Dragana, Kušić-Tišma, Jelena, Divac Rankov, Aleksandra, Tomić, Branko, "Formation of noncanonical DNA structures mediated by human ORC4, a protein component of the origin recognition complex" in Biochemistry, 47, no. 33 (2008):8760-8767, https://doi.org/10.1021/bi800684f . .