Compensatory role for Rad52 during recombinational repair in Ustilago maydis
Само за регистроване кориснике
2008
Чланак у часопису (Објављена верзија)
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Метаподаци
Приказ свих података о документуАпстракт
A single Rad52-related protein is evident by blast analysis of the Ustilago maydis genome database. Mutants created by disruption of the structural gene exhibited few discernible defects in resistance to UV, ionizing radiation, chemical alkylating or cross-linking agents. No deficiency was noted in spontaneous mutator activity, allelic recombination or meiosis. GFP-Rad51 foci were formed in rad52 cells following DNA damage, but were initially less intense than normal suggesting a possible role for Rad52 in formation of the Rad51 nucleoprotein filament. A search for interacting genes that confer a synthetic fitness phenotype with rad52 after DNA damage by UV irradiation identified the genes for Mph1, Ercc1 and the Rad51 paralogue Rec2. Testing known mutants in recombinational repair revealed an additional interaction with the BRCA2 orthologue Brh2. Suppression of the rec2 mutant's UV sensitivity by overexpressing Brh2 was found to be dependent on Rad52. The results suggest that Rad52 se...rves in an overlapping, compensatory role with both Rec2 and Brh2 to promote and maintain formation of the Rad51 nucleoprotein filament.
Извор:
Molecular Microbiology, 2008, 67, 5, 1156-1168Издавач:
- Wiley, Hoboken
Финансирање / пројекти:
- NIGMS NIH HHS [GM42482, GM79859] Funding Source: Medline
- NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES [R01GM042482, R01GM079859] Funding Source: NIH RePORTER
DOI: 10.1111/j.1365-2958.2008.06116.x
ISSN: 0950-382X
PubMed: 18208529
WoS: 000252932000016
Scopus: 2-s2.0-38849159142
Институција/група
Institut za molekularnu genetiku i genetičko inženjerstvoTY - JOUR AU - Kojić, Milorad AU - Mao, Ninghui AU - Zhou, Qingwen AU - Lisby, Michael AU - Holloman, William K. PY - 2008 UR - https://imagine.imgge.bg.ac.rs/handle/123456789/314 AB - A single Rad52-related protein is evident by blast analysis of the Ustilago maydis genome database. Mutants created by disruption of the structural gene exhibited few discernible defects in resistance to UV, ionizing radiation, chemical alkylating or cross-linking agents. No deficiency was noted in spontaneous mutator activity, allelic recombination or meiosis. GFP-Rad51 foci were formed in rad52 cells following DNA damage, but were initially less intense than normal suggesting a possible role for Rad52 in formation of the Rad51 nucleoprotein filament. A search for interacting genes that confer a synthetic fitness phenotype with rad52 after DNA damage by UV irradiation identified the genes for Mph1, Ercc1 and the Rad51 paralogue Rec2. Testing known mutants in recombinational repair revealed an additional interaction with the BRCA2 orthologue Brh2. Suppression of the rec2 mutant's UV sensitivity by overexpressing Brh2 was found to be dependent on Rad52. The results suggest that Rad52 serves in an overlapping, compensatory role with both Rec2 and Brh2 to promote and maintain formation of the Rad51 nucleoprotein filament. PB - Wiley, Hoboken T2 - Molecular Microbiology T1 - Compensatory role for Rad52 during recombinational repair in Ustilago maydis EP - 1168 IS - 5 SP - 1156 VL - 67 DO - 10.1111/j.1365-2958.2008.06116.x ER -
@article{ author = "Kojić, Milorad and Mao, Ninghui and Zhou, Qingwen and Lisby, Michael and Holloman, William K.", year = "2008", abstract = "A single Rad52-related protein is evident by blast analysis of the Ustilago maydis genome database. Mutants created by disruption of the structural gene exhibited few discernible defects in resistance to UV, ionizing radiation, chemical alkylating or cross-linking agents. No deficiency was noted in spontaneous mutator activity, allelic recombination or meiosis. GFP-Rad51 foci were formed in rad52 cells following DNA damage, but were initially less intense than normal suggesting a possible role for Rad52 in formation of the Rad51 nucleoprotein filament. A search for interacting genes that confer a synthetic fitness phenotype with rad52 after DNA damage by UV irradiation identified the genes for Mph1, Ercc1 and the Rad51 paralogue Rec2. Testing known mutants in recombinational repair revealed an additional interaction with the BRCA2 orthologue Brh2. Suppression of the rec2 mutant's UV sensitivity by overexpressing Brh2 was found to be dependent on Rad52. The results suggest that Rad52 serves in an overlapping, compensatory role with both Rec2 and Brh2 to promote and maintain formation of the Rad51 nucleoprotein filament.", publisher = "Wiley, Hoboken", journal = "Molecular Microbiology", title = "Compensatory role for Rad52 during recombinational repair in Ustilago maydis", pages = "1168-1156", number = "5", volume = "67", doi = "10.1111/j.1365-2958.2008.06116.x" }
Kojić, M., Mao, N., Zhou, Q., Lisby, M.,& Holloman, W. K.. (2008). Compensatory role for Rad52 during recombinational repair in Ustilago maydis. in Molecular Microbiology Wiley, Hoboken., 67(5), 1156-1168. https://doi.org/10.1111/j.1365-2958.2008.06116.x
Kojić M, Mao N, Zhou Q, Lisby M, Holloman WK. Compensatory role for Rad52 during recombinational repair in Ustilago maydis. in Molecular Microbiology. 2008;67(5):1156-1168. doi:10.1111/j.1365-2958.2008.06116.x .
Kojić, Milorad, Mao, Ninghui, Zhou, Qingwen, Lisby, Michael, Holloman, William K., "Compensatory role for Rad52 during recombinational repair in Ustilago maydis" in Molecular Microbiology, 67, no. 5 (2008):1156-1168, https://doi.org/10.1111/j.1365-2958.2008.06116.x . .