Aminoglycoside resistance genes sgm and kgmB protect bacterial but not yeast small ribosomal subunits in vitro despite high conservation of the rRNA A-site
Abstract
The aminoglycoside resistance genes sgm from Micromonospora zionensis and kgmB from Streptomyces tenebrarius were cloned into a yeast expression vector to test whether the encoded prokaryotic methylases can modify the 18S rRNA A-site and thus confer resistance to G-418. Despite the detectable presence of mRNAs in yeast cells, neither G-418-resistant yeast transformants nor positive western blot signals were obtained. Neither methylase was capable of methylating 40S subunits despite very high conservation of the antibiotic rRNA binding sites. However, the results provide novel insight into the action of Sgm by showing that it methylates the same site as KgmB, i.e. G1405 in 16S rRNA.
Keywords:
Site of action / G1405 / Expression in yeast / Aminoglycoside resistance / 16S rRNA methylasesSource:
Research in Microbiology, 2008, 159, 9-10, 658-662Publisher:
- Elsevier Science Bv, Amsterdam
Funding / projects:
- Ekspresija i regulacija farmaceutski značajnih gena u mikroorganizmima (RS-MESTD-MPN2006-2010-143056)
- The Wellcome Trust [078374]
DOI: 10.1016/j.resmic.2008.09.006
ISSN: 0923-2508
PubMed: 18930134
WoS: 000262129900010
Scopus: 2-s2.0-57049159208
Collections
Institution/Community
Institut za molekularnu genetiku i genetičko inženjerstvoTY - JOUR AU - Ilić-Tomić, Tatjana AU - Morić, Ivana AU - Conn, Graeme L. AU - Vasiljević, Branka PY - 2008 UR - https://imagine.imgge.bg.ac.rs/handle/123456789/324 AB - The aminoglycoside resistance genes sgm from Micromonospora zionensis and kgmB from Streptomyces tenebrarius were cloned into a yeast expression vector to test whether the encoded prokaryotic methylases can modify the 18S rRNA A-site and thus confer resistance to G-418. Despite the detectable presence of mRNAs in yeast cells, neither G-418-resistant yeast transformants nor positive western blot signals were obtained. Neither methylase was capable of methylating 40S subunits despite very high conservation of the antibiotic rRNA binding sites. However, the results provide novel insight into the action of Sgm by showing that it methylates the same site as KgmB, i.e. G1405 in 16S rRNA. PB - Elsevier Science Bv, Amsterdam T2 - Research in Microbiology T1 - Aminoglycoside resistance genes sgm and kgmB protect bacterial but not yeast small ribosomal subunits in vitro despite high conservation of the rRNA A-site EP - 662 IS - 9-10 SP - 658 VL - 159 DO - 10.1016/j.resmic.2008.09.006 ER -
@article{ author = "Ilić-Tomić, Tatjana and Morić, Ivana and Conn, Graeme L. and Vasiljević, Branka", year = "2008", abstract = "The aminoglycoside resistance genes sgm from Micromonospora zionensis and kgmB from Streptomyces tenebrarius were cloned into a yeast expression vector to test whether the encoded prokaryotic methylases can modify the 18S rRNA A-site and thus confer resistance to G-418. Despite the detectable presence of mRNAs in yeast cells, neither G-418-resistant yeast transformants nor positive western blot signals were obtained. Neither methylase was capable of methylating 40S subunits despite very high conservation of the antibiotic rRNA binding sites. However, the results provide novel insight into the action of Sgm by showing that it methylates the same site as KgmB, i.e. G1405 in 16S rRNA.", publisher = "Elsevier Science Bv, Amsterdam", journal = "Research in Microbiology", title = "Aminoglycoside resistance genes sgm and kgmB protect bacterial but not yeast small ribosomal subunits in vitro despite high conservation of the rRNA A-site", pages = "662-658", number = "9-10", volume = "159", doi = "10.1016/j.resmic.2008.09.006" }
Ilić-Tomić, T., Morić, I., Conn, G. L.,& Vasiljević, B.. (2008). Aminoglycoside resistance genes sgm and kgmB protect bacterial but not yeast small ribosomal subunits in vitro despite high conservation of the rRNA A-site. in Research in Microbiology Elsevier Science Bv, Amsterdam., 159(9-10), 658-662. https://doi.org/10.1016/j.resmic.2008.09.006
Ilić-Tomić T, Morić I, Conn GL, Vasiljević B. Aminoglycoside resistance genes sgm and kgmB protect bacterial but not yeast small ribosomal subunits in vitro despite high conservation of the rRNA A-site. in Research in Microbiology. 2008;159(9-10):658-662. doi:10.1016/j.resmic.2008.09.006 .
Ilić-Tomić, Tatjana, Morić, Ivana, Conn, Graeme L., Vasiljević, Branka, "Aminoglycoside resistance genes sgm and kgmB protect bacterial but not yeast small ribosomal subunits in vitro despite high conservation of the rRNA A-site" in Research in Microbiology, 159, no. 9-10 (2008):658-662, https://doi.org/10.1016/j.resmic.2008.09.006 . .