In vivo localizations of membrane stress controllers PspA and PspG in Escherichia coli
2009
Аутори
Engl, ChristophJovanović, Goran
Lloyd, Louise J.
Murray, Heath
Spitaler, Martin
Ying, Liming
Errington, Jeff
Buck, Martin
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
The phage shock protein (Psp) response in Gram-negative bacteria counteracts membrane stress. Transcription of the PspF regulon (pspABCDE and pspG) in Escherichia coli is induced upon stresses that dissipate the proton motive force (pmf). Using GFP fusions we have visualized the subcellular localizations of PspA (a negative regulator and effector of Psp) and PspG (an effector of Psp). It has previously been proposed that PspA evenly coates the inner membrane of the cell. We now demonstrate that instead of uniformly covering the entire cell, PspA (and PspG) is highly organized into what appear to be distinct functional classes (complexes at the cell pole and the lateral cell wall). Real-time observations revealed lateral PspA and PspG complexes are highly mobile, but absent in cells lacking MreB. Without the MreB cytoskeleton, induction of the Psp response is still observed, yet these cells fail to maintain pmf under stress conditions. The two spatial subspecies therefore appear to be d...ynamically and functionally distinct with the polar clusters being associated with sensory function and the mobile complexes with maintenance of pmf.
Извор:
Molecular Microbiology, 2009, 73, 3, 382-396Издавач:
- Wiley, Hoboken
Финансирање / пројекти:
- The Wellcome Trus
- BBSRC [43/G18654]
- European Molecular Biology Organization (EMBO)
- Human Frontier Science Program (HFSP)
- BBSRC [BB/G00594X/1] Funding Source: UKRI
- Biotechnology and Biological Sciences Research Council [BB/G00594X/1] Funding Source: researchfish
DOI: 10.1111/j.1365-2958.2009.06776.x
ISSN: 0950-382X
PubMed: 19555453
WoS: 000268792700006
Scopus: 2-s2.0-67651216196
Институција/група
Institut za molekularnu genetiku i genetičko inženjerstvoTY - JOUR AU - Engl, Christoph AU - Jovanović, Goran AU - Lloyd, Louise J. AU - Murray, Heath AU - Spitaler, Martin AU - Ying, Liming AU - Errington, Jeff AU - Buck, Martin PY - 2009 UR - https://imagine.imgge.bg.ac.rs/handle/123456789/354 AB - The phage shock protein (Psp) response in Gram-negative bacteria counteracts membrane stress. Transcription of the PspF regulon (pspABCDE and pspG) in Escherichia coli is induced upon stresses that dissipate the proton motive force (pmf). Using GFP fusions we have visualized the subcellular localizations of PspA (a negative regulator and effector of Psp) and PspG (an effector of Psp). It has previously been proposed that PspA evenly coates the inner membrane of the cell. We now demonstrate that instead of uniformly covering the entire cell, PspA (and PspG) is highly organized into what appear to be distinct functional classes (complexes at the cell pole and the lateral cell wall). Real-time observations revealed lateral PspA and PspG complexes are highly mobile, but absent in cells lacking MreB. Without the MreB cytoskeleton, induction of the Psp response is still observed, yet these cells fail to maintain pmf under stress conditions. The two spatial subspecies therefore appear to be dynamically and functionally distinct with the polar clusters being associated with sensory function and the mobile complexes with maintenance of pmf. PB - Wiley, Hoboken T2 - Molecular Microbiology T1 - In vivo localizations of membrane stress controllers PspA and PspG in Escherichia coli EP - 396 IS - 3 SP - 382 VL - 73 DO - 10.1111/j.1365-2958.2009.06776.x ER -
@article{ author = "Engl, Christoph and Jovanović, Goran and Lloyd, Louise J. and Murray, Heath and Spitaler, Martin and Ying, Liming and Errington, Jeff and Buck, Martin", year = "2009", abstract = "The phage shock protein (Psp) response in Gram-negative bacteria counteracts membrane stress. Transcription of the PspF regulon (pspABCDE and pspG) in Escherichia coli is induced upon stresses that dissipate the proton motive force (pmf). Using GFP fusions we have visualized the subcellular localizations of PspA (a negative regulator and effector of Psp) and PspG (an effector of Psp). It has previously been proposed that PspA evenly coates the inner membrane of the cell. We now demonstrate that instead of uniformly covering the entire cell, PspA (and PspG) is highly organized into what appear to be distinct functional classes (complexes at the cell pole and the lateral cell wall). Real-time observations revealed lateral PspA and PspG complexes are highly mobile, but absent in cells lacking MreB. Without the MreB cytoskeleton, induction of the Psp response is still observed, yet these cells fail to maintain pmf under stress conditions. The two spatial subspecies therefore appear to be dynamically and functionally distinct with the polar clusters being associated with sensory function and the mobile complexes with maintenance of pmf.", publisher = "Wiley, Hoboken", journal = "Molecular Microbiology", title = "In vivo localizations of membrane stress controllers PspA and PspG in Escherichia coli", pages = "396-382", number = "3", volume = "73", doi = "10.1111/j.1365-2958.2009.06776.x" }
Engl, C., Jovanović, G., Lloyd, L. J., Murray, H., Spitaler, M., Ying, L., Errington, J.,& Buck, M.. (2009). In vivo localizations of membrane stress controllers PspA and PspG in Escherichia coli. in Molecular Microbiology Wiley, Hoboken., 73(3), 382-396. https://doi.org/10.1111/j.1365-2958.2009.06776.x
Engl C, Jovanović G, Lloyd LJ, Murray H, Spitaler M, Ying L, Errington J, Buck M. In vivo localizations of membrane stress controllers PspA and PspG in Escherichia coli. in Molecular Microbiology. 2009;73(3):382-396. doi:10.1111/j.1365-2958.2009.06776.x .
Engl, Christoph, Jovanović, Goran, Lloyd, Louise J., Murray, Heath, Spitaler, Martin, Ying, Liming, Errington, Jeff, Buck, Martin, "In vivo localizations of membrane stress controllers PspA and PspG in Escherichia coli" in Molecular Microbiology, 73, no. 3 (2009):382-396, https://doi.org/10.1111/j.1365-2958.2009.06776.x . .