Functional analysis of the buckwheat metallothionein promoter: Tissue specificity pattern and up-regulation under complex stress stimuli
Samo za registrovane korisnike
2009
Članak u časopisu (Objavljena verzija)
Metapodaci
Prikaz svih podataka o dokumentuApstrakt
To shed light on expression regulation of the metallothionein gene from buckwheat (FeMT3), functional promoter analysis was performed with a complete 5' regulatory region and two deletion variants, employing stably transformed tobacco plants. Histochemical GUS assay of transgenic tobacco lines showed the strongest. signals in vascular elements of leaves and in pollen grains, while somewhat weaker staining was observed in the roots of mature plants. This tissue specificity pattern implies a possible function of buckwheat MT3 in those tissues. Quantitative GUS assay showed strong up-regulation of all three promoter constructs (proportional to the length of the regulatory region) in leaves submerged in liquid MS medium containing sucrose, after a prolonged time period. This represented a complex stress situation composed of several synergistically related stress stimuli. These findings suggest: complex transcriptional regulation of FeMT3, requiring interactions among a number of different... factors.
Ključne reči:
Transformation / Stress / Promoter / Metallothionein / BuckwheatIzvor:
Journal of Plant Physiology, 2009, 166, 9, 996-1000Izdavač:
- Elsevier Gmbh, Urban & Fischer Verlag, Jena
Finansiranje / projekti:
- Struktura, funkcija i regulacija ekspresije odabranih biljnih gena (RS-MESTD-MPN2006-2010-143017)
DOI: 10.1016/j.jplph.2008.12.002
ISSN: 0176-1617
PubMed: 19185389
WoS: 000266416900011
Scopus: 2-s2.0-67349194030
Institucija/grupa
Institut za molekularnu genetiku i genetičko inženjerstvoTY - JOUR AU - Bratić, Ana M. AU - Nikolić, Dragana AU - Samardžić, Jelena AU - Maksimović, Vesna R. PY - 2009 UR - https://imagine.imgge.bg.ac.rs/handle/123456789/355 AB - To shed light on expression regulation of the metallothionein gene from buckwheat (FeMT3), functional promoter analysis was performed with a complete 5' regulatory region and two deletion variants, employing stably transformed tobacco plants. Histochemical GUS assay of transgenic tobacco lines showed the strongest. signals in vascular elements of leaves and in pollen grains, while somewhat weaker staining was observed in the roots of mature plants. This tissue specificity pattern implies a possible function of buckwheat MT3 in those tissues. Quantitative GUS assay showed strong up-regulation of all three promoter constructs (proportional to the length of the regulatory region) in leaves submerged in liquid MS medium containing sucrose, after a prolonged time period. This represented a complex stress situation composed of several synergistically related stress stimuli. These findings suggest: complex transcriptional regulation of FeMT3, requiring interactions among a number of different factors. PB - Elsevier Gmbh, Urban & Fischer Verlag, Jena T2 - Journal of Plant Physiology T1 - Functional analysis of the buckwheat metallothionein promoter: Tissue specificity pattern and up-regulation under complex stress stimuli EP - 1000 IS - 9 SP - 996 VL - 166 DO - 10.1016/j.jplph.2008.12.002 ER -
@article{ author = "Bratić, Ana M. and Nikolić, Dragana and Samardžić, Jelena and Maksimović, Vesna R.", year = "2009", abstract = "To shed light on expression regulation of the metallothionein gene from buckwheat (FeMT3), functional promoter analysis was performed with a complete 5' regulatory region and two deletion variants, employing stably transformed tobacco plants. Histochemical GUS assay of transgenic tobacco lines showed the strongest. signals in vascular elements of leaves and in pollen grains, while somewhat weaker staining was observed in the roots of mature plants. This tissue specificity pattern implies a possible function of buckwheat MT3 in those tissues. Quantitative GUS assay showed strong up-regulation of all three promoter constructs (proportional to the length of the regulatory region) in leaves submerged in liquid MS medium containing sucrose, after a prolonged time period. This represented a complex stress situation composed of several synergistically related stress stimuli. These findings suggest: complex transcriptional regulation of FeMT3, requiring interactions among a number of different factors.", publisher = "Elsevier Gmbh, Urban & Fischer Verlag, Jena", journal = "Journal of Plant Physiology", title = "Functional analysis of the buckwheat metallothionein promoter: Tissue specificity pattern and up-regulation under complex stress stimuli", pages = "1000-996", number = "9", volume = "166", doi = "10.1016/j.jplph.2008.12.002" }
Bratić, A. M., Nikolić, D., Samardžić, J.,& Maksimović, V. R.. (2009). Functional analysis of the buckwheat metallothionein promoter: Tissue specificity pattern and up-regulation under complex stress stimuli. in Journal of Plant Physiology Elsevier Gmbh, Urban & Fischer Verlag, Jena., 166(9), 996-1000. https://doi.org/10.1016/j.jplph.2008.12.002
Bratić AM, Nikolić D, Samardžić J, Maksimović VR. Functional analysis of the buckwheat metallothionein promoter: Tissue specificity pattern and up-regulation under complex stress stimuli. in Journal of Plant Physiology. 2009;166(9):996-1000. doi:10.1016/j.jplph.2008.12.002 .
Bratić, Ana M., Nikolić, Dragana, Samardžić, Jelena, Maksimović, Vesna R., "Functional analysis of the buckwheat metallothionein promoter: Tissue specificity pattern and up-regulation under complex stress stimuli" in Journal of Plant Physiology, 166, no. 9 (2009):996-1000, https://doi.org/10.1016/j.jplph.2008.12.002 . .