Basic RNases of wild almond (Prunus webbii): Cloning and characterization of six new S-RNase and one "non-S RNase" genes
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2009
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In order to investigate the S-RNase allele structure of a Prunus webbii population from the Montenegrin region of the Balkans, we analyzed 10 Prunus webbii accessions. We detected 10 different S-RNase allelic variants and obtained the nucleotide sequences for six S-RNases. The BLAST analysis showed that these six sequences were new Prunus webbii S-RNase alleles. It also revealed that one of sequenced alleles, S(9)-RNase, coded for an amino acid sequence identical to that for Prunus dulcis S(14)-RNase, except for a single conservative amino acid replacement in the signal peptide region. Another, S(3)-RNase, was shown to differ by only three amino acid residues from Prunus salicina Se-RNase. The allele S(7)-RNase was found to be inactive by stylar protein isoelectric focusing followed by RNase-specific staining, but the reason for the inactivity was not at the coding sequence level. Further, in five of the 10 analyzed accessions, we detected the presence of one active basic RNase (marked... PW(1)) that did not amplify with S-RNase-specific DNA primers. However, it was amplified with primers designed from the PA1 RNase nucleotide sequence (basic "non-S RNase" of Prunus avium) and the obtained sequence showed high homology (80%) with the PA1 allele. Although homologs of PA1 "non-S RNases" have been reported in four other Prunus species, this is the first recorded homolog in Prunus webbii. The evolutionary implications of the data are discussed.
Ključne reči:
S-RNases / Self-incompatibility / Prunus webbii / Basic non-S RNasesIzvor:
Journal of Plant Physiology, 2009, 166, 4, 395-402Izdavač:
- Elsevier Gmbh, Urban & Fischer Verlag, Jena
Finansiranje / projekti:
- Struktura, funkcija i regulacija ekspresije odabranih biljnih gena (RS-MESTD-MPN2006-2010-143017)
DOI: 10.1016/j.jplph.2008.06.009
ISSN: 0176-1617
PubMed: 18778875
WoS: 000263691700007
Scopus: 2-s2.0-59349090580
Institucija/grupa
Institut za molekularnu genetiku i genetičko inženjerstvoTY - JOUR AU - Banović Đeri, Bojana AU - Surbanovski, Nada AU - Konstantinović, Miroslav AU - Maksimović, Vesna R. PY - 2009 UR - https://imagine.imgge.bg.ac.rs/handle/123456789/362 AB - In order to investigate the S-RNase allele structure of a Prunus webbii population from the Montenegrin region of the Balkans, we analyzed 10 Prunus webbii accessions. We detected 10 different S-RNase allelic variants and obtained the nucleotide sequences for six S-RNases. The BLAST analysis showed that these six sequences were new Prunus webbii S-RNase alleles. It also revealed that one of sequenced alleles, S(9)-RNase, coded for an amino acid sequence identical to that for Prunus dulcis S(14)-RNase, except for a single conservative amino acid replacement in the signal peptide region. Another, S(3)-RNase, was shown to differ by only three amino acid residues from Prunus salicina Se-RNase. The allele S(7)-RNase was found to be inactive by stylar protein isoelectric focusing followed by RNase-specific staining, but the reason for the inactivity was not at the coding sequence level. Further, in five of the 10 analyzed accessions, we detected the presence of one active basic RNase (marked PW(1)) that did not amplify with S-RNase-specific DNA primers. However, it was amplified with primers designed from the PA1 RNase nucleotide sequence (basic "non-S RNase" of Prunus avium) and the obtained sequence showed high homology (80%) with the PA1 allele. Although homologs of PA1 "non-S RNases" have been reported in four other Prunus species, this is the first recorded homolog in Prunus webbii. The evolutionary implications of the data are discussed. PB - Elsevier Gmbh, Urban & Fischer Verlag, Jena T2 - Journal of Plant Physiology T1 - Basic RNases of wild almond (Prunus webbii): Cloning and characterization of six new S-RNase and one "non-S RNase" genes EP - 402 IS - 4 SP - 395 VL - 166 DO - 10.1016/j.jplph.2008.06.009 ER -
@article{ author = "Banović Đeri, Bojana and Surbanovski, Nada and Konstantinović, Miroslav and Maksimović, Vesna R.", year = "2009", abstract = "In order to investigate the S-RNase allele structure of a Prunus webbii population from the Montenegrin region of the Balkans, we analyzed 10 Prunus webbii accessions. We detected 10 different S-RNase allelic variants and obtained the nucleotide sequences for six S-RNases. The BLAST analysis showed that these six sequences were new Prunus webbii S-RNase alleles. It also revealed that one of sequenced alleles, S(9)-RNase, coded for an amino acid sequence identical to that for Prunus dulcis S(14)-RNase, except for a single conservative amino acid replacement in the signal peptide region. Another, S(3)-RNase, was shown to differ by only three amino acid residues from Prunus salicina Se-RNase. The allele S(7)-RNase was found to be inactive by stylar protein isoelectric focusing followed by RNase-specific staining, but the reason for the inactivity was not at the coding sequence level. Further, in five of the 10 analyzed accessions, we detected the presence of one active basic RNase (marked PW(1)) that did not amplify with S-RNase-specific DNA primers. However, it was amplified with primers designed from the PA1 RNase nucleotide sequence (basic "non-S RNase" of Prunus avium) and the obtained sequence showed high homology (80%) with the PA1 allele. Although homologs of PA1 "non-S RNases" have been reported in four other Prunus species, this is the first recorded homolog in Prunus webbii. The evolutionary implications of the data are discussed.", publisher = "Elsevier Gmbh, Urban & Fischer Verlag, Jena", journal = "Journal of Plant Physiology", title = "Basic RNases of wild almond (Prunus webbii): Cloning and characterization of six new S-RNase and one "non-S RNase" genes", pages = "402-395", number = "4", volume = "166", doi = "10.1016/j.jplph.2008.06.009" }
Banović Đeri, B., Surbanovski, N., Konstantinović, M.,& Maksimović, V. R.. (2009). Basic RNases of wild almond (Prunus webbii): Cloning and characterization of six new S-RNase and one "non-S RNase" genes. in Journal of Plant Physiology Elsevier Gmbh, Urban & Fischer Verlag, Jena., 166(4), 395-402. https://doi.org/10.1016/j.jplph.2008.06.009
Banović Đeri B, Surbanovski N, Konstantinović M, Maksimović VR. Basic RNases of wild almond (Prunus webbii): Cloning and characterization of six new S-RNase and one "non-S RNase" genes. in Journal of Plant Physiology. 2009;166(4):395-402. doi:10.1016/j.jplph.2008.06.009 .
Banović Đeri, Bojana, Surbanovski, Nada, Konstantinović, Miroslav, Maksimović, Vesna R., "Basic RNases of wild almond (Prunus webbii): Cloning and characterization of six new S-RNase and one "non-S RNase" genes" in Journal of Plant Physiology, 166, no. 4 (2009):395-402, https://doi.org/10.1016/j.jplph.2008.06.009 . .