Evaluation of antimicrobial and proteolytic activity of enterococci isolated from fermented products
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2009
Authors
Veljović, KatarinaFira, Đorđe
Terzić-Vidojević, Amarela
Abriouel, Hikmate
Galvez, Antonio
Topisirović, Ljubiša
Article (Published version)
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A collection of 26 enterococci isolated from dairy and meat products were tested for antimicrobial and proteolytic activity. Enterococcus faecium and E. faecalis were the most frequent species among tested enterococci, and 11 isolates produced antimicrobial compounds. Results revealed that 10 out of 11 enterococci synthesized enterocins showing antimicrobial activity against food-born pathogen such as Listeria monocytogenes and Staphylococcus aureus. The broadest spectrum of antimicrobial activity was detected in E. faecalis BGPT1-10P and BGPT1-78. E. faecalis BG221 showed antimicrobial activity that was not related to production of enterocin, H2O2 or organic acid. Twenty-five enterococci showed strong or moderate proteolytic activity towards beta-casein. Two isolates, BGPT1-10P and BGPT1-78, showed the most intense hydrolysis of alpha(s1)-, beta-, kappa-casein fractions, total casein as well as gelatin. Extracellular BGPT1-10P and BGPT1-78 proteinases have a molecular mass of about 29... kDa. Bacteriocin production and proteinase activity of natural isolates of enterococci may be of technological interest in dairy and meat-fermented products.
Keywords:
Proteolytic activity / Enterococci / Enterocins / Antimicrobial activitySource:
European Food Research and Technology, 2009, 230, 1, 63-70Publisher:
- Springer, New York
Funding / projects:
- Izučavanje regulacije ekspresije gena odabranih industrijskih mikroorganizama (RS-MESTD-MPN2006-2010-143036)
DOI: 10.1007/s00217-009-1137-6
ISSN: 1438-2377
WoS: 000270870600006
Scopus: 2-s2.0-70350362176
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Institut za molekularnu genetiku i genetičko inženjerstvoTY - JOUR AU - Veljović, Katarina AU - Fira, Đorđe AU - Terzić-Vidojević, Amarela AU - Abriouel, Hikmate AU - Galvez, Antonio AU - Topisirović, Ljubiša PY - 2009 UR - https://imagine.imgge.bg.ac.rs/handle/123456789/378 AB - A collection of 26 enterococci isolated from dairy and meat products were tested for antimicrobial and proteolytic activity. Enterococcus faecium and E. faecalis were the most frequent species among tested enterococci, and 11 isolates produced antimicrobial compounds. Results revealed that 10 out of 11 enterococci synthesized enterocins showing antimicrobial activity against food-born pathogen such as Listeria monocytogenes and Staphylococcus aureus. The broadest spectrum of antimicrobial activity was detected in E. faecalis BGPT1-10P and BGPT1-78. E. faecalis BG221 showed antimicrobial activity that was not related to production of enterocin, H2O2 or organic acid. Twenty-five enterococci showed strong or moderate proteolytic activity towards beta-casein. Two isolates, BGPT1-10P and BGPT1-78, showed the most intense hydrolysis of alpha(s1)-, beta-, kappa-casein fractions, total casein as well as gelatin. Extracellular BGPT1-10P and BGPT1-78 proteinases have a molecular mass of about 29 kDa. Bacteriocin production and proteinase activity of natural isolates of enterococci may be of technological interest in dairy and meat-fermented products. PB - Springer, New York T2 - European Food Research and Technology T1 - Evaluation of antimicrobial and proteolytic activity of enterococci isolated from fermented products EP - 70 IS - 1 SP - 63 VL - 230 DO - 10.1007/s00217-009-1137-6 ER -
@article{ author = "Veljović, Katarina and Fira, Đorđe and Terzić-Vidojević, Amarela and Abriouel, Hikmate and Galvez, Antonio and Topisirović, Ljubiša", year = "2009", abstract = "A collection of 26 enterococci isolated from dairy and meat products were tested for antimicrobial and proteolytic activity. Enterococcus faecium and E. faecalis were the most frequent species among tested enterococci, and 11 isolates produced antimicrobial compounds. Results revealed that 10 out of 11 enterococci synthesized enterocins showing antimicrobial activity against food-born pathogen such as Listeria monocytogenes and Staphylococcus aureus. The broadest spectrum of antimicrobial activity was detected in E. faecalis BGPT1-10P and BGPT1-78. E. faecalis BG221 showed antimicrobial activity that was not related to production of enterocin, H2O2 or organic acid. Twenty-five enterococci showed strong or moderate proteolytic activity towards beta-casein. Two isolates, BGPT1-10P and BGPT1-78, showed the most intense hydrolysis of alpha(s1)-, beta-, kappa-casein fractions, total casein as well as gelatin. Extracellular BGPT1-10P and BGPT1-78 proteinases have a molecular mass of about 29 kDa. Bacteriocin production and proteinase activity of natural isolates of enterococci may be of technological interest in dairy and meat-fermented products.", publisher = "Springer, New York", journal = "European Food Research and Technology", title = "Evaluation of antimicrobial and proteolytic activity of enterococci isolated from fermented products", pages = "70-63", number = "1", volume = "230", doi = "10.1007/s00217-009-1137-6" }
Veljović, K., Fira, Đ., Terzić-Vidojević, A., Abriouel, H., Galvez, A.,& Topisirović, L.. (2009). Evaluation of antimicrobial and proteolytic activity of enterococci isolated from fermented products. in European Food Research and Technology Springer, New York., 230(1), 63-70. https://doi.org/10.1007/s00217-009-1137-6
Veljović K, Fira Đ, Terzić-Vidojević A, Abriouel H, Galvez A, Topisirović L. Evaluation of antimicrobial and proteolytic activity of enterococci isolated from fermented products. in European Food Research and Technology. 2009;230(1):63-70. doi:10.1007/s00217-009-1137-6 .
Veljović, Katarina, Fira, Đorđe, Terzić-Vidojević, Amarela, Abriouel, Hikmate, Galvez, Antonio, Topisirović, Ljubiša, "Evaluation of antimicrobial and proteolytic activity of enterococci isolated from fermented products" in European Food Research and Technology, 230, no. 1 (2009):63-70, https://doi.org/10.1007/s00217-009-1137-6 . .