Incidenca hiperhomocisteinemije i polimorfizam MTHFR C677T kod mladih bolesnika sa akutnim infarktom miokarda
Incidence of hyperhomocysteinemia and MTHFR C677T polymorphism among young patients with acute myocardial infarction
2009
Authors
Beletić, AnđeloMirković, Duško
Antonijević, Nebojša
Đorđević, Valentina
Šango, Violeta
Jakovljević, Branko
Peruničić, Jovan
Ilić, Mirka
Vasiljević, Zorana
Majkić-Singh, Nada
Article (Published version)
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Hiperhomocisteinemija se smatra nezavisnim faktorom rizika za preuranjeni razvoj kardiovaskularnih bolesti. Mutacija MTHFR C677T snižava aktivnost metilentetra hidrofol atreduktaze i može dovesti do hiperhomocisteinemije. Incidenca hiperhomocisteinemije (homocisteinemija iz nad 12 μmol/L), nivo homocisteina i raspodela MTHFR 677 genotipova (C/C,C/T,T/T) upoređeni su između mladih bolesnika sa akutnim infarktom miokarda i zdravih osoba iste dobi. Studija je obuhvatila 86 bolesnika mlađih od 45 godina (77 muškaraca i 9 žena) i kontrolnu grupu od 35 osoba. Homocistein je određivan metodom HPLC, a MTHFR 677 genotip PCR amplifikacijom i digestijom sa Hinf I. Podaci su statistički obrađeni pomoću Chi-square i Mann-Whitney U testa. Hiper homocisteinemija je bila prisutna kod 32,6% bolesnika i 14,3% zdravih osoba, što pred stavlja statistički značajnu razliku (P=0,038). Medijane homocisteinemija bolesnika (10,4 μmol/L) i zdravih osoba (9,6 μmol/L) bile su statistički značajno različite (P= 0,0...35). Raspodela MTHFR 677 genotipova kod bolesnika (50,0% C/C, 41,9% C/T i 8,1% T/T) nije se statistički značajno razlikovala od raspodele u kontrolnoj grupi. Genotip MTHFR 677 nije uticao na incidencu hiperhomocisteinemije i nivo homocisteina kod bolesnika. Može se zaključiti da mlađi bolesnici sa akutnim infarktom miokarda imaju višu incidencu hiperhomocisteinemije i viši nivo homocisteina nego zdrave osobe iste starosti, pri čemu nema značajne razlike u raspodeli geno tipova MTHFR.
Hyperhomocysteinemia is considered an independent risk factor for premature cardiovascular disease. Mutation MTHFR C677T reduces the activity of methylene tetrahydrofolatereductase and may cause hyperhomocysteinemia. Incidence of hyperhomocysteinemia (homocysteine above 12 μmol/L), ho mocysteine level, and distribution of MTHFR C677T genotypes (C/C, C/T and T/T) are compared between young patients with acute myocardial infarction and healthy persons, matched by age. Study involved 86 patients younger than 45 years (77 men and 9 women) and 35 controls. Homocysteine was measured by an HPLC method and the MTHFR C677T genotype determined using PCR amplification and digestion with Hinf I. Statistical analyses included chisquare and Mann-Whitney U tests. Hyperhomocysteinemia was present in 32.6% patients and 14.3% controls, revealing a significant difference (P= 0.038). Median homocysteine levels in patients (10.4 μmol/L) and controls (9.6 μmol/L) were significantly different (P=0.035). Amon...g patients, 50.0% had C/C, 41.9% C/T and 8.1% T/T genotype, and the genotype had no influence on hyperhomocysteinemia incidence and homocysteine level. Genotype distribution in patients was not significantly different from that observed in controls. The conclusion is that young patients with acute myocardial infarction have higher incidence of hyperhomocysteinemia and higher homocysteine levels than healthy young adults, while there is no significant difference in the distribution of MTHFR C677T genotypes.
Keywords:
MTHFR / mladi bolesnici / infarkt miokarda / hiperhomocisteinemija / young adults / myocardial infarction / MTHFR / hyperhomocysteinemiaSource:
Journal of Medical Biochemistry, 2009, 28, 1, 41-45Publisher:
- Društvo medicinskih biohemičara Srbije, Beograd i Versita
Funding / projects:
- Biohemijski pokazatelji oštećenja i disfunkcije organa (RS-MESTD-MPN2006-2010-145010)
DOI: 10.2478/v10011-008-0029-9
ISSN: 1452-8258
WoS: 000270010700007
Scopus: 2-s2.0-60249088201
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Institution/Community
Institut za molekularnu genetiku i genetičko inženjerstvoTY - JOUR AU - Beletić, Anđelo AU - Mirković, Duško AU - Antonijević, Nebojša AU - Đorđević, Valentina AU - Šango, Violeta AU - Jakovljević, Branko AU - Peruničić, Jovan AU - Ilić, Mirka AU - Vasiljević, Zorana AU - Majkić-Singh, Nada PY - 2009 UR - https://imagine.imgge.bg.ac.rs/handle/123456789/393 AB - Hiperhomocisteinemija se smatra nezavisnim faktorom rizika za preuranjeni razvoj kardiovaskularnih bolesti. Mutacija MTHFR C677T snižava aktivnost metilentetra hidrofol atreduktaze i može dovesti do hiperhomocisteinemije. Incidenca hiperhomocisteinemije (homocisteinemija iz nad 12 μmol/L), nivo homocisteina i raspodela MTHFR 677 genotipova (C/C,C/T,T/T) upoređeni su između mladih bolesnika sa akutnim infarktom miokarda i zdravih osoba iste dobi. Studija je obuhvatila 86 bolesnika mlađih od 45 godina (77 muškaraca i 9 žena) i kontrolnu grupu od 35 osoba. Homocistein je određivan metodom HPLC, a MTHFR 677 genotip PCR amplifikacijom i digestijom sa Hinf I. Podaci su statistički obrađeni pomoću Chi-square i Mann-Whitney U testa. Hiper homocisteinemija je bila prisutna kod 32,6% bolesnika i 14,3% zdravih osoba, što pred stavlja statistički značajnu razliku (P=0,038). Medijane homocisteinemija bolesnika (10,4 μmol/L) i zdravih osoba (9,6 μmol/L) bile su statistički značajno različite (P= 0,035). Raspodela MTHFR 677 genotipova kod bolesnika (50,0% C/C, 41,9% C/T i 8,1% T/T) nije se statistički značajno razlikovala od raspodele u kontrolnoj grupi. Genotip MTHFR 677 nije uticao na incidencu hiperhomocisteinemije i nivo homocisteina kod bolesnika. Može se zaključiti da mlađi bolesnici sa akutnim infarktom miokarda imaju višu incidencu hiperhomocisteinemije i viši nivo homocisteina nego zdrave osobe iste starosti, pri čemu nema značajne razlike u raspodeli geno tipova MTHFR. AB - Hyperhomocysteinemia is considered an independent risk factor for premature cardiovascular disease. Mutation MTHFR C677T reduces the activity of methylene tetrahydrofolatereductase and may cause hyperhomocysteinemia. Incidence of hyperhomocysteinemia (homocysteine above 12 μmol/L), ho mocysteine level, and distribution of MTHFR C677T genotypes (C/C, C/T and T/T) are compared between young patients with acute myocardial infarction and healthy persons, matched by age. Study involved 86 patients younger than 45 years (77 men and 9 women) and 35 controls. Homocysteine was measured by an HPLC method and the MTHFR C677T genotype determined using PCR amplification and digestion with Hinf I. Statistical analyses included chisquare and Mann-Whitney U tests. Hyperhomocysteinemia was present in 32.6% patients and 14.3% controls, revealing a significant difference (P= 0.038). Median homocysteine levels in patients (10.4 μmol/L) and controls (9.6 μmol/L) were significantly different (P=0.035). Among patients, 50.0% had C/C, 41.9% C/T and 8.1% T/T genotype, and the genotype had no influence on hyperhomocysteinemia incidence and homocysteine level. Genotype distribution in patients was not significantly different from that observed in controls. The conclusion is that young patients with acute myocardial infarction have higher incidence of hyperhomocysteinemia and higher homocysteine levels than healthy young adults, while there is no significant difference in the distribution of MTHFR C677T genotypes. PB - Društvo medicinskih biohemičara Srbije, Beograd i Versita T2 - Journal of Medical Biochemistry T1 - Incidenca hiperhomocisteinemije i polimorfizam MTHFR C677T kod mladih bolesnika sa akutnim infarktom miokarda T1 - Incidence of hyperhomocysteinemia and MTHFR C677T polymorphism among young patients with acute myocardial infarction EP - 45 IS - 1 SP - 41 VL - 28 DO - 10.2478/v10011-008-0029-9 ER -
@article{ author = "Beletić, Anđelo and Mirković, Duško and Antonijević, Nebojša and Đorđević, Valentina and Šango, Violeta and Jakovljević, Branko and Peruničić, Jovan and Ilić, Mirka and Vasiljević, Zorana and Majkić-Singh, Nada", year = "2009", abstract = "Hiperhomocisteinemija se smatra nezavisnim faktorom rizika za preuranjeni razvoj kardiovaskularnih bolesti. Mutacija MTHFR C677T snižava aktivnost metilentetra hidrofol atreduktaze i može dovesti do hiperhomocisteinemije. Incidenca hiperhomocisteinemije (homocisteinemija iz nad 12 μmol/L), nivo homocisteina i raspodela MTHFR 677 genotipova (C/C,C/T,T/T) upoređeni su između mladih bolesnika sa akutnim infarktom miokarda i zdravih osoba iste dobi. Studija je obuhvatila 86 bolesnika mlađih od 45 godina (77 muškaraca i 9 žena) i kontrolnu grupu od 35 osoba. Homocistein je određivan metodom HPLC, a MTHFR 677 genotip PCR amplifikacijom i digestijom sa Hinf I. Podaci su statistički obrađeni pomoću Chi-square i Mann-Whitney U testa. Hiper homocisteinemija je bila prisutna kod 32,6% bolesnika i 14,3% zdravih osoba, što pred stavlja statistički značajnu razliku (P=0,038). Medijane homocisteinemija bolesnika (10,4 μmol/L) i zdravih osoba (9,6 μmol/L) bile su statistički značajno različite (P= 0,035). Raspodela MTHFR 677 genotipova kod bolesnika (50,0% C/C, 41,9% C/T i 8,1% T/T) nije se statistički značajno razlikovala od raspodele u kontrolnoj grupi. Genotip MTHFR 677 nije uticao na incidencu hiperhomocisteinemije i nivo homocisteina kod bolesnika. Može se zaključiti da mlađi bolesnici sa akutnim infarktom miokarda imaju višu incidencu hiperhomocisteinemije i viši nivo homocisteina nego zdrave osobe iste starosti, pri čemu nema značajne razlike u raspodeli geno tipova MTHFR., Hyperhomocysteinemia is considered an independent risk factor for premature cardiovascular disease. Mutation MTHFR C677T reduces the activity of methylene tetrahydrofolatereductase and may cause hyperhomocysteinemia. Incidence of hyperhomocysteinemia (homocysteine above 12 μmol/L), ho mocysteine level, and distribution of MTHFR C677T genotypes (C/C, C/T and T/T) are compared between young patients with acute myocardial infarction and healthy persons, matched by age. Study involved 86 patients younger than 45 years (77 men and 9 women) and 35 controls. Homocysteine was measured by an HPLC method and the MTHFR C677T genotype determined using PCR amplification and digestion with Hinf I. Statistical analyses included chisquare and Mann-Whitney U tests. Hyperhomocysteinemia was present in 32.6% patients and 14.3% controls, revealing a significant difference (P= 0.038). Median homocysteine levels in patients (10.4 μmol/L) and controls (9.6 μmol/L) were significantly different (P=0.035). Among patients, 50.0% had C/C, 41.9% C/T and 8.1% T/T genotype, and the genotype had no influence on hyperhomocysteinemia incidence and homocysteine level. Genotype distribution in patients was not significantly different from that observed in controls. The conclusion is that young patients with acute myocardial infarction have higher incidence of hyperhomocysteinemia and higher homocysteine levels than healthy young adults, while there is no significant difference in the distribution of MTHFR C677T genotypes.", publisher = "Društvo medicinskih biohemičara Srbije, Beograd i Versita", journal = "Journal of Medical Biochemistry", title = "Incidenca hiperhomocisteinemije i polimorfizam MTHFR C677T kod mladih bolesnika sa akutnim infarktom miokarda, Incidence of hyperhomocysteinemia and MTHFR C677T polymorphism among young patients with acute myocardial infarction", pages = "45-41", number = "1", volume = "28", doi = "10.2478/v10011-008-0029-9" }
Beletić, A., Mirković, D., Antonijević, N., Đorđević, V., Šango, V., Jakovljević, B., Peruničić, J., Ilić, M., Vasiljević, Z.,& Majkić-Singh, N.. (2009). Incidenca hiperhomocisteinemije i polimorfizam MTHFR C677T kod mladih bolesnika sa akutnim infarktom miokarda. in Journal of Medical Biochemistry Društvo medicinskih biohemičara Srbije, Beograd i Versita., 28(1), 41-45. https://doi.org/10.2478/v10011-008-0029-9
Beletić A, Mirković D, Antonijević N, Đorđević V, Šango V, Jakovljević B, Peruničić J, Ilić M, Vasiljević Z, Majkić-Singh N. Incidenca hiperhomocisteinemije i polimorfizam MTHFR C677T kod mladih bolesnika sa akutnim infarktom miokarda. in Journal of Medical Biochemistry. 2009;28(1):41-45. doi:10.2478/v10011-008-0029-9 .
Beletić, Anđelo, Mirković, Duško, Antonijević, Nebojša, Đorđević, Valentina, Šango, Violeta, Jakovljević, Branko, Peruničić, Jovan, Ilić, Mirka, Vasiljević, Zorana, Majkić-Singh, Nada, "Incidenca hiperhomocisteinemije i polimorfizam MTHFR C677T kod mladih bolesnika sa akutnim infarktom miokarda" in Journal of Medical Biochemistry, 28, no. 1 (2009):41-45, https://doi.org/10.2478/v10011-008-0029-9 . .