Est-ssrs developed for other picea species amplify in picea omorika and reveal high genetic variation in two natural populations
Апстракт
Picea omorika (Pane.) Purk. is a relict and an endemic species found exclusively in the Balkan, within an area of ca. 10 000 km(2). Marker-based genetic diversity data in this conifer are very limited and partially contradictory. Therefore, twelve nuclear markers (ten EST-SSRs and two genomic SSRs) were tested for cross-species amplification in P. omorika. Five EST-SSRs amplified successfully and a very high number of alleles per locus was found in 50 trees originating from two natural populations (7 to 18 alleles per locus), with a total of 61 alleles. Furthermore, a high number of private alleles were detected - 13 and 14 per population, respectively. Rare alleles, i.e., alleles whose frequency was lower than 0.05, were not observed. Markers were selectively neutral, no linkage disequilibrium was detected and the genotype frequencies fitted Hardy-Weinberg proportions. Expected heterozygosity per locus ranged from 0.64 to 0.91 in both populations, with an overall mean of 0.83. Conside...ring the small remnant population sizes of P. omorika, these values are unexpectedly high and comparable to values in P. glauca (Moench) Voss., P. sitchensis (Bong.) Carr. and P. mariana (Mill.) B.S.P. detected with an identical set of markers in samples of similar sizes.
Кључне речи:
Picea omorika / microsatellites / genomic SSRs / genetic diversity / EST-SSRs / endemic speciesИзвор:
Belgian Journal of Botany, 2009, 142, 1, 89-95Издавач:
- Soc Royal Botan Belgique, Meise
Финансирање / пројекти:
- Bioversity International
- Republic of Austria
- Tara National Park, (Serbia)
- Ministry of Agriculture, Forestry and Water Management and Forest Enterprise 'Sume Republike Srpske'
Институција/група
Institut za molekularnu genetiku i genetičko inženjerstvoTY - JOUR AU - Aleksić, Jelena M. AU - Schueler, Silvio AU - Mengl, Michael AU - Geburek, Thomas PY - 2009 UR - https://imagine.imgge.bg.ac.rs/handle/123456789/400 AB - Picea omorika (Pane.) Purk. is a relict and an endemic species found exclusively in the Balkan, within an area of ca. 10 000 km(2). Marker-based genetic diversity data in this conifer are very limited and partially contradictory. Therefore, twelve nuclear markers (ten EST-SSRs and two genomic SSRs) were tested for cross-species amplification in P. omorika. Five EST-SSRs amplified successfully and a very high number of alleles per locus was found in 50 trees originating from two natural populations (7 to 18 alleles per locus), with a total of 61 alleles. Furthermore, a high number of private alleles were detected - 13 and 14 per population, respectively. Rare alleles, i.e., alleles whose frequency was lower than 0.05, were not observed. Markers were selectively neutral, no linkage disequilibrium was detected and the genotype frequencies fitted Hardy-Weinberg proportions. Expected heterozygosity per locus ranged from 0.64 to 0.91 in both populations, with an overall mean of 0.83. Considering the small remnant population sizes of P. omorika, these values are unexpectedly high and comparable to values in P. glauca (Moench) Voss., P. sitchensis (Bong.) Carr. and P. mariana (Mill.) B.S.P. detected with an identical set of markers in samples of similar sizes. PB - Soc Royal Botan Belgique, Meise T2 - Belgian Journal of Botany T1 - Est-ssrs developed for other picea species amplify in picea omorika and reveal high genetic variation in two natural populations EP - 95 IS - 1 SP - 89 VL - 142 UR - https://hdl.handle.net/21.15107/rcub_imagine_400 ER -
@article{ author = "Aleksić, Jelena M. and Schueler, Silvio and Mengl, Michael and Geburek, Thomas", year = "2009", abstract = "Picea omorika (Pane.) Purk. is a relict and an endemic species found exclusively in the Balkan, within an area of ca. 10 000 km(2). Marker-based genetic diversity data in this conifer are very limited and partially contradictory. Therefore, twelve nuclear markers (ten EST-SSRs and two genomic SSRs) were tested for cross-species amplification in P. omorika. Five EST-SSRs amplified successfully and a very high number of alleles per locus was found in 50 trees originating from two natural populations (7 to 18 alleles per locus), with a total of 61 alleles. Furthermore, a high number of private alleles were detected - 13 and 14 per population, respectively. Rare alleles, i.e., alleles whose frequency was lower than 0.05, were not observed. Markers were selectively neutral, no linkage disequilibrium was detected and the genotype frequencies fitted Hardy-Weinberg proportions. Expected heterozygosity per locus ranged from 0.64 to 0.91 in both populations, with an overall mean of 0.83. Considering the small remnant population sizes of P. omorika, these values are unexpectedly high and comparable to values in P. glauca (Moench) Voss., P. sitchensis (Bong.) Carr. and P. mariana (Mill.) B.S.P. detected with an identical set of markers in samples of similar sizes.", publisher = "Soc Royal Botan Belgique, Meise", journal = "Belgian Journal of Botany", title = "Est-ssrs developed for other picea species amplify in picea omorika and reveal high genetic variation in two natural populations", pages = "95-89", number = "1", volume = "142", url = "https://hdl.handle.net/21.15107/rcub_imagine_400" }
Aleksić, J. M., Schueler, S., Mengl, M.,& Geburek, T.. (2009). Est-ssrs developed for other picea species amplify in picea omorika and reveal high genetic variation in two natural populations. in Belgian Journal of Botany Soc Royal Botan Belgique, Meise., 142(1), 89-95. https://hdl.handle.net/21.15107/rcub_imagine_400
Aleksić JM, Schueler S, Mengl M, Geburek T. Est-ssrs developed for other picea species amplify in picea omorika and reveal high genetic variation in two natural populations. in Belgian Journal of Botany. 2009;142(1):89-95. https://hdl.handle.net/21.15107/rcub_imagine_400 .
Aleksić, Jelena M., Schueler, Silvio, Mengl, Michael, Geburek, Thomas, "Est-ssrs developed for other picea species amplify in picea omorika and reveal high genetic variation in two natural populations" in Belgian Journal of Botany, 142, no. 1 (2009):89-95, https://hdl.handle.net/21.15107/rcub_imagine_400 .