Ubiquitous aspartic proteinase as an actor in the stress response in buckwheat
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2010
Authors
Timotijević, GordanaMilisavljević, Mira
Radović, Svetlana R.
Konstantinović, Miroslav M.
Maksimović, Vesna R.
Article (Published version)
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Show full item recordAbstract
The aspartic protease (FeAP9) gene from buckwheat resembles the exon-intron structure characteristic for typical aspartic proteinases, including the presence of the leader intron in the 5'-UTR. RT PCR experiments and gel protein blot analysis indicated that FeAP9 was present in all analyzed organs: developing seeds, seedlings, flowers, leaves, roots and stems. Using Real-time PCR, we found that FeAP9 expression is upregulated in buckwheat leaves under the influence of different abiotic stresses, including dark, drought and UV-B light, as well as wounding and salicylic acid.
Keywords:
Leader intron / Gene expression / Buckwheat / Aspartic proteinase / Abiotic stressSource:
Journal of Plant Physiology, 2010, 167, 1, 61-68Publisher:
- Elsevier Gmbh, Urban & Fischer Verlag, Jena
Funding / projects:
- Struktura, funkcija i regulacija ekspresije odabranih biljnih gena (RS-MESTD-MPN2006-2010-143017)
DOI: 10.1016/j.jplph.2009.06.017
ISSN: 0176-1617
PubMed: 19643510
WoS: 000273708300010
Scopus: 2-s2.0-70450242789
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Institut za molekularnu genetiku i genetičko inženjerstvoTY - JOUR AU - Timotijević, Gordana AU - Milisavljević, Mira AU - Radović, Svetlana R. AU - Konstantinović, Miroslav M. AU - Maksimović, Vesna R. PY - 2010 UR - https://imagine.imgge.bg.ac.rs/handle/123456789/409 AB - The aspartic protease (FeAP9) gene from buckwheat resembles the exon-intron structure characteristic for typical aspartic proteinases, including the presence of the leader intron in the 5'-UTR. RT PCR experiments and gel protein blot analysis indicated that FeAP9 was present in all analyzed organs: developing seeds, seedlings, flowers, leaves, roots and stems. Using Real-time PCR, we found that FeAP9 expression is upregulated in buckwheat leaves under the influence of different abiotic stresses, including dark, drought and UV-B light, as well as wounding and salicylic acid. PB - Elsevier Gmbh, Urban & Fischer Verlag, Jena T2 - Journal of Plant Physiology T1 - Ubiquitous aspartic proteinase as an actor in the stress response in buckwheat EP - 68 IS - 1 SP - 61 VL - 167 DO - 10.1016/j.jplph.2009.06.017 ER -
@article{ author = "Timotijević, Gordana and Milisavljević, Mira and Radović, Svetlana R. and Konstantinović, Miroslav M. and Maksimović, Vesna R.", year = "2010", abstract = "The aspartic protease (FeAP9) gene from buckwheat resembles the exon-intron structure characteristic for typical aspartic proteinases, including the presence of the leader intron in the 5'-UTR. RT PCR experiments and gel protein blot analysis indicated that FeAP9 was present in all analyzed organs: developing seeds, seedlings, flowers, leaves, roots and stems. Using Real-time PCR, we found that FeAP9 expression is upregulated in buckwheat leaves under the influence of different abiotic stresses, including dark, drought and UV-B light, as well as wounding and salicylic acid.", publisher = "Elsevier Gmbh, Urban & Fischer Verlag, Jena", journal = "Journal of Plant Physiology", title = "Ubiquitous aspartic proteinase as an actor in the stress response in buckwheat", pages = "68-61", number = "1", volume = "167", doi = "10.1016/j.jplph.2009.06.017" }
Timotijević, G., Milisavljević, M., Radović, S. R., Konstantinović, M. M.,& Maksimović, V. R.. (2010). Ubiquitous aspartic proteinase as an actor in the stress response in buckwheat. in Journal of Plant Physiology Elsevier Gmbh, Urban & Fischer Verlag, Jena., 167(1), 61-68. https://doi.org/10.1016/j.jplph.2009.06.017
Timotijević G, Milisavljević M, Radović SR, Konstantinović MM, Maksimović VR. Ubiquitous aspartic proteinase as an actor in the stress response in buckwheat. in Journal of Plant Physiology. 2010;167(1):61-68. doi:10.1016/j.jplph.2009.06.017 .
Timotijević, Gordana, Milisavljević, Mira, Radović, Svetlana R., Konstantinović, Miroslav M., Maksimović, Vesna R., "Ubiquitous aspartic proteinase as an actor in the stress response in buckwheat" in Journal of Plant Physiology, 167, no. 1 (2010):61-68, https://doi.org/10.1016/j.jplph.2009.06.017 . .