Buckwheat (Fagopyrum esculentum Moench) FeMT3 Gene in Heavy Metal Stress: Protective Role of the Protein and Inducibility of the Promoter Region under Cu2+ and Cd2+ Treatments
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2010
Authors
Nikolić, DraganaSamardžić, Jelena
Bratić, Ana M.
Radin, Ivan P.
Gavrilović, Srdjan P.
Rausch, Thomas
Maksimović, Vesna R.
Article (Published version)
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The protective role in vivo of buckwheat metallothionein type 3 (FeMT3) during metal stress and the responsiveness of its promoter to metal ions were examined. Increased tolerance to heavy metals of FeMT3 producing Escherichia coli and cup1(Delta) yeast cells was detected. The defensive ability of buckwheat MT3 during Cd and Cu stresses was also demonstrated in Nicotiana debneyii leaves transiently expressing FeMT3. In contrast to phytochelatins, the cytoplasmatic localization of FeMT3 was not altered under heavy metal stress. Functional analysis of the corresponding promoter region revealed extremely high inducibility upon Cu2+ and Cd2+ treatments. The confirmed defense ability of FeMT3 protein in vivo and the great responsiveness of its promoter during heavy metal exposure make this gene a suitable candidate for biotechnological applications.
Keywords:
overexpression / metailothionein / heavy metal tolerance / Buckwheat (Fagopyrum esculentum Moench)Source:
Journal of Agricultural and Food Chemistry, 2010, 58, 6, 3488-3494Publisher:
- Amer Chemical Soc, Washington
Funding / projects:
- Struktura, funkcija i regulacija ekspresije odabranih biljnih gena (RS-MESTD-MPN2006-2010-143017)
DOI: 10.1021/jf904483a
ISSN: 0021-8561
PubMed: 20187605
WoS: 000275710700035
Scopus: 2-s2.0-77949820670
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Institut za molekularnu genetiku i genetičko inženjerstvoTY - JOUR AU - Nikolić, Dragana AU - Samardžić, Jelena AU - Bratić, Ana M. AU - Radin, Ivan P. AU - Gavrilović, Srdjan P. AU - Rausch, Thomas AU - Maksimović, Vesna R. PY - 2010 UR - https://imagine.imgge.bg.ac.rs/handle/123456789/417 AB - The protective role in vivo of buckwheat metallothionein type 3 (FeMT3) during metal stress and the responsiveness of its promoter to metal ions were examined. Increased tolerance to heavy metals of FeMT3 producing Escherichia coli and cup1(Delta) yeast cells was detected. The defensive ability of buckwheat MT3 during Cd and Cu stresses was also demonstrated in Nicotiana debneyii leaves transiently expressing FeMT3. In contrast to phytochelatins, the cytoplasmatic localization of FeMT3 was not altered under heavy metal stress. Functional analysis of the corresponding promoter region revealed extremely high inducibility upon Cu2+ and Cd2+ treatments. The confirmed defense ability of FeMT3 protein in vivo and the great responsiveness of its promoter during heavy metal exposure make this gene a suitable candidate for biotechnological applications. PB - Amer Chemical Soc, Washington T2 - Journal of Agricultural and Food Chemistry T1 - Buckwheat (Fagopyrum esculentum Moench) FeMT3 Gene in Heavy Metal Stress: Protective Role of the Protein and Inducibility of the Promoter Region under Cu2+ and Cd2+ Treatments EP - 3494 IS - 6 SP - 3488 VL - 58 DO - 10.1021/jf904483a ER -
@article{ author = "Nikolić, Dragana and Samardžić, Jelena and Bratić, Ana M. and Radin, Ivan P. and Gavrilović, Srdjan P. and Rausch, Thomas and Maksimović, Vesna R.", year = "2010", abstract = "The protective role in vivo of buckwheat metallothionein type 3 (FeMT3) during metal stress and the responsiveness of its promoter to metal ions were examined. Increased tolerance to heavy metals of FeMT3 producing Escherichia coli and cup1(Delta) yeast cells was detected. The defensive ability of buckwheat MT3 during Cd and Cu stresses was also demonstrated in Nicotiana debneyii leaves transiently expressing FeMT3. In contrast to phytochelatins, the cytoplasmatic localization of FeMT3 was not altered under heavy metal stress. Functional analysis of the corresponding promoter region revealed extremely high inducibility upon Cu2+ and Cd2+ treatments. The confirmed defense ability of FeMT3 protein in vivo and the great responsiveness of its promoter during heavy metal exposure make this gene a suitable candidate for biotechnological applications.", publisher = "Amer Chemical Soc, Washington", journal = "Journal of Agricultural and Food Chemistry", title = "Buckwheat (Fagopyrum esculentum Moench) FeMT3 Gene in Heavy Metal Stress: Protective Role of the Protein and Inducibility of the Promoter Region under Cu2+ and Cd2+ Treatments", pages = "3494-3488", number = "6", volume = "58", doi = "10.1021/jf904483a" }
Nikolić, D., Samardžić, J., Bratić, A. M., Radin, I. P., Gavrilović, S. P., Rausch, T.,& Maksimović, V. R.. (2010). Buckwheat (Fagopyrum esculentum Moench) FeMT3 Gene in Heavy Metal Stress: Protective Role of the Protein and Inducibility of the Promoter Region under Cu2+ and Cd2+ Treatments. in Journal of Agricultural and Food Chemistry Amer Chemical Soc, Washington., 58(6), 3488-3494. https://doi.org/10.1021/jf904483a
Nikolić D, Samardžić J, Bratić AM, Radin IP, Gavrilović SP, Rausch T, Maksimović VR. Buckwheat (Fagopyrum esculentum Moench) FeMT3 Gene in Heavy Metal Stress: Protective Role of the Protein and Inducibility of the Promoter Region under Cu2+ and Cd2+ Treatments. in Journal of Agricultural and Food Chemistry. 2010;58(6):3488-3494. doi:10.1021/jf904483a .
Nikolić, Dragana, Samardžić, Jelena, Bratić, Ana M., Radin, Ivan P., Gavrilović, Srdjan P., Rausch, Thomas, Maksimović, Vesna R., "Buckwheat (Fagopyrum esculentum Moench) FeMT3 Gene in Heavy Metal Stress: Protective Role of the Protein and Inducibility of the Promoter Region under Cu2+ and Cd2+ Treatments" in Journal of Agricultural and Food Chemistry, 58, no. 6 (2010):3488-3494, https://doi.org/10.1021/jf904483a . .