Zinc-induced oxidative stress in Verbascum thapsus is caused by an accumulation of reactive oxygen species and quinhydrone in the cell wall
Samo za registrovane korisnike
2010
Autori
Morina, FilisJovanović, Ljubinko
Mojović, Milos
Vidović, Marija
Panković, Dejana
Veljović-Jovanović, Sonja
Članak u časopisu (Objavljena verzija)
Metapodaci
Prikaz svih podataka o dokumentuApstrakt
Oxidative stress is one aspect of metal toxicity. Zinc, although unable to perform univalent oxido-reduction reactions, can induce the oxidative damage of cellular components and alter antioxidative systems. Verbascum thapsus L. plants that were grown hydroponically were exposed to 1 and 5 mM Zn2+. Reactive oxygen species (ROS) accumulation was demonstrated by the fluorescent probe H(2)DCFDA and EPR measurements. The extent of zinc-induced oxidative damage was assessed by measuring the level of protein carbonylation. Activities and isoform profiles of some antioxidant enzymes and the changes in ascorbate and total phenolic contents of leaves and roots were determined. Stunted growth because of zinc accumulation, preferentially in the roots, was accompanied by H2O2 production in the leaf and root apoplasts. Increased EPR signals of the endogenous oxidant quinhydrone, center dot CH3 and center dot OH, were found in the cell walls of zinc-treated plants. The activities of the antioxidativ...e enzymes ascorbate peroxidase (APX) (EC 1.11.1.11), soluble superoxide dismutase (SOD) (EC 1.15.1.1), peroxidase (POD), (EC 1.11.1.7) and monodehydroascorbate reductase (EC 1.6.5.4) were increased; those of glutathione reductase (EC 1.6.4.2), dehydroascorbate reductase (EC 1.8.5.1) and ascorbate oxidase (AAO) (EC 1.10.3.3) were decreased with zinc treatment. Zinc induced a cell-wall-bound SOD isoform in both organs. Leaves accumulated more ascorbate and phenolics in comparison to roots. We propose a mechanism for zinc-promoted oxidative stress in V. thapsus L. through the generation of charge transfer complexes and quinhydrone because of phenoxyl radical stabilisation by Zn2+ in the cell wall. Our results suggest that the SOD and APX responses are mediated by ROS accumulation in the apoplast. The importance of the POD/Phe/AA (ascorbic acid) scavenging system in the apoplast is also discussed.
Ključne reči:
cell-wall-bound superoxide dismutase (SOD) / charge transfer complexes / quinhydrone / zinc-promoted oxidative stress / reactive oxygen species / cell wallIzvor:
Physiologia Plantarum, 2010, 140, 3, 209-224Izdavač:
- Wiley, Hoboken
Finansiranje / projekti:
- Regulacija antioksidativnog metabolizma biljaka u toku rastenja, infekcije patogenima i delovanja abiotičkog stresa: mehanizmi transporta, signalizacije i otpornosti (RS-MESTD-MPN2006-2010-143020)
DOI: 10.1111/j.1399-3054.2010.01399.x
ISSN: 0031-9317
PubMed: 20626644
WoS: 000282874100001
Scopus: 2-s2.0-78649397055
Institucija/grupa
Institut za molekularnu genetiku i genetičko inženjerstvoTY - JOUR AU - Morina, Filis AU - Jovanović, Ljubinko AU - Mojović, Milos AU - Vidović, Marija AU - Panković, Dejana AU - Veljović-Jovanović, Sonja PY - 2010 UR - https://imagine.imgge.bg.ac.rs/handle/123456789/440 AB - Oxidative stress is one aspect of metal toxicity. Zinc, although unable to perform univalent oxido-reduction reactions, can induce the oxidative damage of cellular components and alter antioxidative systems. Verbascum thapsus L. plants that were grown hydroponically were exposed to 1 and 5 mM Zn2+. Reactive oxygen species (ROS) accumulation was demonstrated by the fluorescent probe H(2)DCFDA and EPR measurements. The extent of zinc-induced oxidative damage was assessed by measuring the level of protein carbonylation. Activities and isoform profiles of some antioxidant enzymes and the changes in ascorbate and total phenolic contents of leaves and roots were determined. Stunted growth because of zinc accumulation, preferentially in the roots, was accompanied by H2O2 production in the leaf and root apoplasts. Increased EPR signals of the endogenous oxidant quinhydrone, center dot CH3 and center dot OH, were found in the cell walls of zinc-treated plants. The activities of the antioxidative enzymes ascorbate peroxidase (APX) (EC 1.11.1.11), soluble superoxide dismutase (SOD) (EC 1.15.1.1), peroxidase (POD), (EC 1.11.1.7) and monodehydroascorbate reductase (EC 1.6.5.4) were increased; those of glutathione reductase (EC 1.6.4.2), dehydroascorbate reductase (EC 1.8.5.1) and ascorbate oxidase (AAO) (EC 1.10.3.3) were decreased with zinc treatment. Zinc induced a cell-wall-bound SOD isoform in both organs. Leaves accumulated more ascorbate and phenolics in comparison to roots. We propose a mechanism for zinc-promoted oxidative stress in V. thapsus L. through the generation of charge transfer complexes and quinhydrone because of phenoxyl radical stabilisation by Zn2+ in the cell wall. Our results suggest that the SOD and APX responses are mediated by ROS accumulation in the apoplast. The importance of the POD/Phe/AA (ascorbic acid) scavenging system in the apoplast is also discussed. PB - Wiley, Hoboken T2 - Physiologia Plantarum T1 - Zinc-induced oxidative stress in Verbascum thapsus is caused by an accumulation of reactive oxygen species and quinhydrone in the cell wall EP - 224 IS - 3 SP - 209 VL - 140 DO - 10.1111/j.1399-3054.2010.01399.x ER -
@article{ author = "Morina, Filis and Jovanović, Ljubinko and Mojović, Milos and Vidović, Marija and Panković, Dejana and Veljović-Jovanović, Sonja", year = "2010", abstract = "Oxidative stress is one aspect of metal toxicity. Zinc, although unable to perform univalent oxido-reduction reactions, can induce the oxidative damage of cellular components and alter antioxidative systems. Verbascum thapsus L. plants that were grown hydroponically were exposed to 1 and 5 mM Zn2+. Reactive oxygen species (ROS) accumulation was demonstrated by the fluorescent probe H(2)DCFDA and EPR measurements. The extent of zinc-induced oxidative damage was assessed by measuring the level of protein carbonylation. Activities and isoform profiles of some antioxidant enzymes and the changes in ascorbate and total phenolic contents of leaves and roots were determined. Stunted growth because of zinc accumulation, preferentially in the roots, was accompanied by H2O2 production in the leaf and root apoplasts. Increased EPR signals of the endogenous oxidant quinhydrone, center dot CH3 and center dot OH, were found in the cell walls of zinc-treated plants. The activities of the antioxidative enzymes ascorbate peroxidase (APX) (EC 1.11.1.11), soluble superoxide dismutase (SOD) (EC 1.15.1.1), peroxidase (POD), (EC 1.11.1.7) and monodehydroascorbate reductase (EC 1.6.5.4) were increased; those of glutathione reductase (EC 1.6.4.2), dehydroascorbate reductase (EC 1.8.5.1) and ascorbate oxidase (AAO) (EC 1.10.3.3) were decreased with zinc treatment. Zinc induced a cell-wall-bound SOD isoform in both organs. Leaves accumulated more ascorbate and phenolics in comparison to roots. We propose a mechanism for zinc-promoted oxidative stress in V. thapsus L. through the generation of charge transfer complexes and quinhydrone because of phenoxyl radical stabilisation by Zn2+ in the cell wall. Our results suggest that the SOD and APX responses are mediated by ROS accumulation in the apoplast. The importance of the POD/Phe/AA (ascorbic acid) scavenging system in the apoplast is also discussed.", publisher = "Wiley, Hoboken", journal = "Physiologia Plantarum", title = "Zinc-induced oxidative stress in Verbascum thapsus is caused by an accumulation of reactive oxygen species and quinhydrone in the cell wall", pages = "224-209", number = "3", volume = "140", doi = "10.1111/j.1399-3054.2010.01399.x" }
Morina, F., Jovanović, L., Mojović, M., Vidović, M., Panković, D.,& Veljović-Jovanović, S.. (2010). Zinc-induced oxidative stress in Verbascum thapsus is caused by an accumulation of reactive oxygen species and quinhydrone in the cell wall. in Physiologia Plantarum Wiley, Hoboken., 140(3), 209-224. https://doi.org/10.1111/j.1399-3054.2010.01399.x
Morina F, Jovanović L, Mojović M, Vidović M, Panković D, Veljović-Jovanović S. Zinc-induced oxidative stress in Verbascum thapsus is caused by an accumulation of reactive oxygen species and quinhydrone in the cell wall. in Physiologia Plantarum. 2010;140(3):209-224. doi:10.1111/j.1399-3054.2010.01399.x .
Morina, Filis, Jovanović, Ljubinko, Mojović, Milos, Vidović, Marija, Panković, Dejana, Veljović-Jovanović, Sonja, "Zinc-induced oxidative stress in Verbascum thapsus is caused by an accumulation of reactive oxygen species and quinhydrone in the cell wall" in Physiologia Plantarum, 140, no. 3 (2010):209-224, https://doi.org/10.1111/j.1399-3054.2010.01399.x . .