Mitochondrial DNA reveals complex genetic structuring in a stenoendemic conifer Picea omorika [(Pan.) Purk.] caused by its long persistence within the refugial Balkan region
Само за регистроване кориснике
2010
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Picea omorika (Pan.) Purk. is a relict from the Arcto-Tertiary flora with its entire current natural range confined to an area of only 10,000 km(2) within the Balkans, a region well known as a Quaternary refugium. We have amplified the second intron of the mitochondrial NADH dehydrogenase subunit1 gene in 200 trees originating from ten natural populations to assess the phylogeographic structure and history of this conifer. Five haplotypes harbouring different numbers of 34-bp minisatellites were detected, revealing haplotypic richness of 3.007 and gene diversities H (S) = 0.075 and H (T) = 0.225. More interestingly, despite the very small distribution range of P. omorika and its dispersal by wind, non-random distribution of haplotypes was observed, resulting in an unexpectedly high estimate of population differentiation (G (ST) = 0.668), and 56.8% of molecular variation assigned to variation among populations. Those findings suggest substantial isolation of populations and their partit...ioning into two gene pools characterized by different history and levels of genetic diversity, and very limited seed flow in this species (Nm = 0.25). They support the hypothesised early arrival of P. omorika in the Balkan region, and residence within this refugium during several ice ages at least. We demonstrate that the assessment of genetic diversity and structuring are not straightforward in species confined to refugial regions, and that past microvicariance might bias formal phylogeographic (G (ST) = N (ST) = 0.668) and isolation-by-distance analysis (r = 0.028, P gt 0.05).
Кључне речи:
Serbian spruce / Seed dispersal / Phylogeography / Mitochondrial marker / Genetic diversity / Ecological features / Balkan refugiumИзвор:
Plant Systematics and Evolution, 2010, 285, 1-2, 1-11Издавач:
- Springer Wien, Wien
Финансирање / пројекти:
- Republic of Austria and Bioversity International
- Tara National Park (Serbia)
- Ministry of Agriculture, Forestry and Water Management
- Forest Enterprise Sume Republike Srpske (Republic of Srpska, Bosnia and Herzegovina)
DOI: 10.1007/s00606-009-0250-0
ISSN: 0378-2697
WoS: 000275658900001
Scopus: 2-s2.0-77949542257
Институција/група
Institut za molekularnu genetiku i genetičko inženjerstvoTY - JOUR AU - Aleksić, Jelena M. AU - Geburek, Thomas PY - 2010 UR - https://imagine.imgge.bg.ac.rs/handle/123456789/460 AB - Picea omorika (Pan.) Purk. is a relict from the Arcto-Tertiary flora with its entire current natural range confined to an area of only 10,000 km(2) within the Balkans, a region well known as a Quaternary refugium. We have amplified the second intron of the mitochondrial NADH dehydrogenase subunit1 gene in 200 trees originating from ten natural populations to assess the phylogeographic structure and history of this conifer. Five haplotypes harbouring different numbers of 34-bp minisatellites were detected, revealing haplotypic richness of 3.007 and gene diversities H (S) = 0.075 and H (T) = 0.225. More interestingly, despite the very small distribution range of P. omorika and its dispersal by wind, non-random distribution of haplotypes was observed, resulting in an unexpectedly high estimate of population differentiation (G (ST) = 0.668), and 56.8% of molecular variation assigned to variation among populations. Those findings suggest substantial isolation of populations and their partitioning into two gene pools characterized by different history and levels of genetic diversity, and very limited seed flow in this species (Nm = 0.25). They support the hypothesised early arrival of P. omorika in the Balkan region, and residence within this refugium during several ice ages at least. We demonstrate that the assessment of genetic diversity and structuring are not straightforward in species confined to refugial regions, and that past microvicariance might bias formal phylogeographic (G (ST) = N (ST) = 0.668) and isolation-by-distance analysis (r = 0.028, P gt 0.05). PB - Springer Wien, Wien T2 - Plant Systematics and Evolution T1 - Mitochondrial DNA reveals complex genetic structuring in a stenoendemic conifer Picea omorika [(Pan.) Purk.] caused by its long persistence within the refugial Balkan region EP - 11 IS - 1-2 SP - 1 VL - 285 DO - 10.1007/s00606-009-0250-0 ER -
@article{ author = "Aleksić, Jelena M. and Geburek, Thomas", year = "2010", abstract = "Picea omorika (Pan.) Purk. is a relict from the Arcto-Tertiary flora with its entire current natural range confined to an area of only 10,000 km(2) within the Balkans, a region well known as a Quaternary refugium. We have amplified the second intron of the mitochondrial NADH dehydrogenase subunit1 gene in 200 trees originating from ten natural populations to assess the phylogeographic structure and history of this conifer. Five haplotypes harbouring different numbers of 34-bp minisatellites were detected, revealing haplotypic richness of 3.007 and gene diversities H (S) = 0.075 and H (T) = 0.225. More interestingly, despite the very small distribution range of P. omorika and its dispersal by wind, non-random distribution of haplotypes was observed, resulting in an unexpectedly high estimate of population differentiation (G (ST) = 0.668), and 56.8% of molecular variation assigned to variation among populations. Those findings suggest substantial isolation of populations and their partitioning into two gene pools characterized by different history and levels of genetic diversity, and very limited seed flow in this species (Nm = 0.25). They support the hypothesised early arrival of P. omorika in the Balkan region, and residence within this refugium during several ice ages at least. We demonstrate that the assessment of genetic diversity and structuring are not straightforward in species confined to refugial regions, and that past microvicariance might bias formal phylogeographic (G (ST) = N (ST) = 0.668) and isolation-by-distance analysis (r = 0.028, P gt 0.05).", publisher = "Springer Wien, Wien", journal = "Plant Systematics and Evolution", title = "Mitochondrial DNA reveals complex genetic structuring in a stenoendemic conifer Picea omorika [(Pan.) Purk.] caused by its long persistence within the refugial Balkan region", pages = "11-1", number = "1-2", volume = "285", doi = "10.1007/s00606-009-0250-0" }
Aleksić, J. M.,& Geburek, T.. (2010). Mitochondrial DNA reveals complex genetic structuring in a stenoendemic conifer Picea omorika [(Pan.) Purk.] caused by its long persistence within the refugial Balkan region. in Plant Systematics and Evolution Springer Wien, Wien., 285(1-2), 1-11. https://doi.org/10.1007/s00606-009-0250-0
Aleksić JM, Geburek T. Mitochondrial DNA reveals complex genetic structuring in a stenoendemic conifer Picea omorika [(Pan.) Purk.] caused by its long persistence within the refugial Balkan region. in Plant Systematics and Evolution. 2010;285(1-2):1-11. doi:10.1007/s00606-009-0250-0 .
Aleksić, Jelena M., Geburek, Thomas, "Mitochondrial DNA reveals complex genetic structuring in a stenoendemic conifer Picea omorika [(Pan.) Purk.] caused by its long persistence within the refugial Balkan region" in Plant Systematics and Evolution, 285, no. 1-2 (2010):1-11, https://doi.org/10.1007/s00606-009-0250-0 . .